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    METHOD OF FORMING BARRIER LAYER AND METHOD OF FABRICATING INTERCONNECT
    21.
    发明申请
    METHOD OF FORMING BARRIER LAYER AND METHOD OF FABRICATING INTERCONNECT 审中-公开
    形成障碍层的方法和制作互连的方法

    公开(公告)号：US20060128146A1

    公开(公告)日：2006-06-15

    申请号：US11163435

    申请日：2005-10-19

    Applicant: Chia-Lin Hsu ,  Shu-Jen Chen ,  Jih-Cheng Yeh ,  Chih-Chiang Wu

    Inventor： Chia-Lin Hsu ,  Shu-Jen Chen ,  Jih-Cheng Yeh ,  Chih-Chiang Wu

    IPC: H01L21/44

    CPC classification number: H01L21/2855 ,  H01L21/76843

    Abstract: A method of fabricating a barrier layer is described. A material layer having an opening formed therein is provided. Then, the material layer is disposed inside a physical vapor deposition chamber and a first deposition process is performed to form a first barrier layer on the surface of the opening. The first deposition process includes turning on a plasma source and turning off the plasma source. The turning on of the plasma source and the turning off of the plasma source are separated from each other by an interval less than 2 seconds. Thereafter, the first deposition process is repeated several times to form a second barrier layer comprising a plurality of first barrier layers.

    Abstract translation: 描述制造阻挡层的方法。 提供其中形成有开口的材料层。 然后，将材料层设置在物理气相沉积室内部，并执行第一沉积工艺以在开口的表面上形成第一阻挡层。 第一沉积工艺包括打开等离子体源并关闭等离子体源。 等离子体源的导通和等离子体源的关闭彼此间隔小于2秒的间隔。 此后，重复多次第一沉积工艺以形成包括多个第一阻挡层的第二阻挡层。

    Adenovirus and methods of use thereof
    23.
    发明授权
    Adenovirus and methods of use thereof 失效
    腺病毒及其使用方法

    公开(公告)号：US6001557A

    公开(公告)日：1999-12-14

    申请号：US836022

    申请日：1997-08-25

    Applicant: James M. Wilson ,  Krishna J. Fisher ,  Shu-Jen Chen ,  Matthew Weitzman

    Inventor： James M. Wilson ,  Krishna J. Fisher ,  Shu-Jen Chen ,  Matthew Weitzman

    IPC: C12N15/09 ,  A61K35/76 ,  A61K48/00 ,  C12N7/00 ,  C12N15/861 ,  C12Q1/70 ,  C12N15/64 ,  C12N15/86

    CPC classification number: C12N7/00 ,  C12N15/86 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2810/6018 ,  C12N2840/44

    Abstract: A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably, the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.

    Abstract translation: PCT No.PCT / US95 / 14017 Sec。 371日期1997年8月25日第 102（e）日期1997年8月25日PCT提交1995年10月27日PCT公布。 公开号WO96 / 13597 日期1996年5月9日提供了一种重组腺病毒及其生产方法，其在不存在编码病毒基因的序列的情况下，利用包含腺病毒DNA序列的重组穿梭载体用于复制所必需的5'和3'顺式元件和病毒粒子壳化 和与其连接的选择的小基因，以及包含产生性病毒感染所必需的足够的腺病毒基因序列的辅助性腺病毒。 期望的是，辅助基因通过修饰其5'包装序列而被削弱，这有助于从辅助病毒纯化病毒颗粒。

    Multi-Loop Bridge-Type Apparatus for the Collection of Cells
    26.
    发明申请
    Multi-Loop Bridge-Type Apparatus for the Collection of Cells 审中-公开
    用于收集细胞的多回路桥式装置

    公开(公告)号：US20110184315A1

    公开(公告)日：2011-07-28

    申请号：US12787994

    申请日：2010-05-26

    Applicant: Chih-Ping Chen ,  Shu-Jen Chen

    Inventor： Chih-Ping Chen ,  Shu-Jen Chen

    IPC: A61B10/00

    CPC classification number: A61B10/0291 ,  A61B10/0048 ,  A61B10/0283

    Abstract: A multi-loop bridge-type apparatus for the collection of cells is provided. The multi-loop bridge-type apparatus includes a barrel, a plunger, a filter unit, and at least two external ducts. The barrel includes one open end. The filter unit is disposed inside an inner space of the barrel and partitions the inner space of the barrel into a first space and a second space. The open end is structured for connection to the first space. The plunger is coupled with the barrel and is adapted for executing a piston motion along the second space. Each external duct has two ends respectively communicating with the first space and the second space. Each external duct further includes a control valve for controlling the duct to be conducted or shut off.

    Abstract translation: 提供了一种用于收集单元的多回路桥式装置。 多回路桥式装置包括筒，柱塞，过滤器单元和至少两个外部管道。 枪管包括一个开口端。 过滤器单元设置在筒的内部空间内，并且将筒的内部空间分隔成第一空间和第二空间。 开放端被构造成连接到第一空间。 柱塞与筒连接并且适于沿着第二空间执行活塞运动。 每个外部管道具有分别与第一空间和第二空间连通的两个端部。 每个外部管道还包括用于控制导管或切断管道的控制阀。

    METHOD FOR DETECTING MULTIPLE SMALL NUCLEIC ACIDS
    27.
    发明申请
    METHOD FOR DETECTING MULTIPLE SMALL NUCLEIC ACIDS 审中-公开
    检测多种小核酸的方法

    公开(公告)号：US20110159482A1

    公开(公告)日：2011-06-30

    申请号：US12823711

    申请日：2010-06-25

    Applicant: Po-Ling CHANG ,  Yu-Sun CHANG ,  Shu-Jen CHEN ,  Hua-Chien CHEN

    Inventor： Po-Ling CHANG ,  Yu-Sun CHANG ,  Shu-Jen CHEN ,  Hua-Chien CHEN

    IPC: C12Q1/68

    CPC classification number: C12Q1/6816 ,  C12Q2565/137 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2537/162 ,  C12Q2533/107

    Abstract: The present invention discloses a method for simultaneously detecting multiple small nucleic acids, which comprises steps: mixing a specimen, fluorescent probes, and bridge nucleic acids having different lengths to form a tested liquid; hybridizing the mixed short nucleic acid molecules, probes and bridge nucleic acids; adding ligases to enable the ligations of the short nucleic acid molecules and the fluorescent probes with the bridge nucleic acids being the templates; injecting the tested liquid into a capillary, and applying a voltage to the capillary to generate an electrophoresis effect and separate the hybridization products; and using laser to induce different fluorescent rays from different reaction products, and measuring the fluorescent rays, whereby the present invention can simultaneously detect multiple types of short nucleic acid molecules in a single capillary.

    Abstract translation: 本发明公开了一种同时检测多个小核酸的方法，其特征在于包括以下步骤：混合具有不同长度的样品，荧光探针和桥接核酸以形成测试液; 杂交的短核酸分子，探针和桥核酸杂交; 加入连接酶以使短核酸分子和荧光探针与桥核酸作为模板的连接; 将测试液体注入毛细管中，并向毛细管施加电压以产生电泳效应并分离杂交产物; 并使用激光诱导来自不同反应产物的不同荧光，并测量荧光，由此本发明可以在单个毛细管中同时检测多种类型的短核酸分子。

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