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公开(公告)号：US11808690B2
公开(公告)日：2023-11-07
申请号：US18095908
申请日：2023-01-11
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC: G01N15/14
CPC classification number: G01N15/1459 , G01N15/1425 , G01N15/1429 , G01N15/1434 , G01N2015/1402 , G01N2015/142 , G01N2015/149 , G01N2015/1486
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US11698334B2
公开(公告)日：2023-07-11
申请号：US17003605
申请日：2020-08-26
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Matthew Bahr
CPC classification number: G01N15/1434 , G01N15/1459 , G02F1/33 , H01S3/0071 , G01N2015/1006 , G01N2015/145 , G02F2203/18 , G02F2203/24
Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.
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公开(公告)号：US20230144214A1
公开(公告)日：2023-05-11
申请号：US18095908
申请日：2023-01-11
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC: G01N15/14
CPC classification number: G01N15/1459 , G01N15/1434 , G01N15/1425 , G01N15/1429 , G01N2015/1486 , G01N2015/1402 , G01N2015/142 , G01N2015/149
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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24.发明申请公开(公告)号：US20230022846A1
公开(公告)日：2023-01-26
申请号：US17849274
申请日：2022-06-24
Applicant: Becton, Dickinson and Company
Inventor： Jizuo Zou , Keegan Owsley , Matthew Bahr
IPC: G01N15/14
Abstract: Aspects of the present disclosure include methods for determining a photodetector gain correction factor for application to flow cytometer data. Methods according to certain embodiments include detecting light with a light detection system across a horizontal axis of a flow stream, generating data signals in a photodetector channel (e.g., an imaging photodetector channel) of the light detection system at a plurality of positions across the flow stream and calculating a detector gain correction factor for each position across the flow stream in response to the generated data signals. Methods also include applying a detector gain correction factor to data signals from a photodetector channel (e.g., non-imaging photodetector channels) to generate adjusted signal intensities. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodetector (e.g., an imaging photodetector) for practicing the subject methods are also described. Non-transitory computer readable storage medium and integrated circuits (e.g., FPGAs) are also provided.
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公开(公告)号：US11275026B2
公开(公告)日：2022-03-15
申请号：US16861724
申请日：2020-04-29
Applicant: Becton, Dickinson and Company
Inventor： Matthew Bahr , Eric D. Diebold , Majid Mehrpouyan , Keegan Owsley
Abstract: Aspects of the present disclosure include methods for phase correcting signals from a light detection system (e.g., in a flow cytometer). Methods according to certain embodiments include detecting light from a sample having particles in a flow stream with a light detection system that includes a brightfield photodetector configured to generate a brightfield data signal and a fluorescence detector configured to generate a fluorescence data signal and calculating a phase correction for the fluorescence detector based on the relative phase between the brightfield data signal and the fluorescence data signal. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate a phase correction for a fluorescence detector based on a brightfield data signal and a fluorescence data signal from the fluorescence detector are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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Patent Agency Ranking
公开(公告)号：US10976236B2
公开(公告)日：2021-04-13
申请号：US16817103
申请日：2020-03-12
Applicant: Becton, Dickinson and Company
Inventor： Jizuo Zou , Matthew Bahr , Eric D. Diebold
Abstract: Light detection systems for measuring light (e.g., in a flow stream) are described. Light detection systems according to embodiments include a light scatter detector, a brightfield photodetector and an optical adjustment component configured to convey light to the light scatter detector and to the brightfield photodetector. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having a light scatter detector, a brightfield photodetector and a beam splitter component are also provided.
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公开(公告)号：US10823658B2
公开(公告)日：2020-11-03
申请号：US15992097
申请日：2018-05-29
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Matthew Bahr
Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.
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28.发明申请公开(公告)号：US20200309671A1
公开(公告)日：2020-10-01
申请号：US16817080
申请日：2020-03-12
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Matthew Bahr , Keegan Owsley
Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.
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公开(公告)号：US20250035535A1
公开(公告)日：2025-01-30
申请号：US18817809
申请日：2024-08-28
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC: G01N15/14 , G01N15/1404 , G01N15/1429 , G01N15/1434 , G01N15/149
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US20230057188A1
公开(公告)日：2023-02-23
申请号：US17955060
申请日：2022-09-28
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Jonathan Lin , Matthew Bahr , Keegan Owsley
Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.
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