公开(公告)号：US20190309372A1

公开(公告)日：2019-10-10

申请号：US16315405

申请日：2017-07-05

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Helen Moinova ,  Amitabh Chak ,  Joseph Willis ,  Thomas LaFramboise

IPC: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6853

Abstract: The disclosure provides methods for identifying genomic loci (e.g., vimentin and/or SqBE18) that are differentially methylated in metaplasias (e.g., Barrett's esophagus) and/or neoplastic cancers (e.g., esophageal cancers). Identification of methylated genomic loci has numerous uses, including for example, to characterize disease risk, to predict responsiveness to therapy, to non-invasively diagnose subjects and to treat subjects determined to have gastrointestinal metaplasias and/or neoplasias.

公开(公告)号：US20130131329A1

公开(公告)日：2013-05-23

申请号：US13670155

申请日：2012-11-06

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Joseph Willis ,  Amitabh Chak ,  Rom Leidner

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6886 ,  C07H21/04 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/136 ,  C12Q2600/154 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as neoplasia of the upper or lower gastrointestinal tract, pancreas, and/or bladder.

Abstract translation: 本申请描述了用于检测和治疗波形蛋白相关瘤形成的方法和组合物。 已经观察到波形蛋白核苷酸序列的差异甲基化在波形蛋白相关瘤形成中，例如上，下胃肠道，胰腺和/或膀胱的瘤形成。

公开(公告)号：US20240068045A1

公开(公告)日：2024-02-29

申请号：US18381926

申请日：2023-10-19

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Joseph E. Willis ,  Helen Moinova ,  Thomas LaFramboise ,  Omar de la Cruz Cabrera ,  Amitabh Chak

IPC: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/686

CPC classification number: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The disclosure provides methods for identifying genomic loci that are differentially methylated in neoplastic cancers, e.g., esophageal cancers. Identification of methylated genomic loci, and optionally in combination with the identification of somatic mutations in TP53, has numerous uses, including for example, to characterize disease risk, to predict responsiveness to therapy, to non-invasively diagnose subjects and to treat subjects determined to have gastrointestinal neoplasias.

公开(公告)号：US20240068033A1

公开(公告)日：2024-02-29

申请号：US18261366

申请日：2022-01-14

Applicant: CASE WESTERN RESERVE UNIVERSITY ,  THE JOHNS HOPKINS UNIVERSITY

Inventor： Sanford Markowitz ,  Amitabh Chak ,  Helen Moinova ,  Joseph Willis ,  Bert Vogelstein ,  Kenneth Kinzler ,  Nickolas Papadopoulos ,  Chetan Bettewgowda ,  Christopher Douville

IPC: C12Q1/6883

CPC classification number: C12Q1/6883 ,  C12Q2600/118 ,  C12Q2600/156

Abstract: A method of detecting Barrett's esophagus with low grade dysplasia, or Barrett's esophagus with high grade dysplasia, or adenocarcinoma of the esophagus, applying a Repetitive Element Aneuploidy Sequencing System (RealSeqS) methodology to a biological sample from the esophagus of the subject to detect Barrett's esophagus with low grade dysplasia, or Barrett's esophagus with high grade dysplasia, or adenocarcinoma of the esophagus.

公开(公告)号：US20210386410A1

公开(公告)日：2021-12-16

申请号：US16902579

申请日：2020-06-16

Applicant: Case Western Reserve University

Inventor： Amitabh Chak ,  Sanford Markowitz ,  Dennis Siedlak ,  Joseph Willis ,  Lishan Aklog ,  Michael Boutillette ,  Richard Yazbeck

IPC: A61B10/02 ,  A61M25/10

Abstract: A device for collecting a biological sample in a subject, the device including an inflatable portion attached to a tubular member, and designed to expand from within the tubular member when inflated and to retract within the tubular member, a surface on the inflatable portion provided with varied thickness to facilitate movement of the inflatable portion between an expanded state and a retracted state, and a plurality of projections on the surface to allow collection of the biological sample when the inflatable portion is in the expanded state.

公开(公告)号：US20210369256A1

公开(公告)日：2021-12-02

申请号：US17331375

申请日：2021-05-26

Applicant: Case Western Reserve University

Inventor： Sanford Markowitz ,  Amitabh Chak ,  Joseph Willis ,  Lishan Aklog ,  Richard Yazbeck ,  Michael Boutillette ,  Brian deGuzman ,  David Wurtman

IPC: A61B10/04

Abstract: A method for collecting a cell sample, including eosinophil white blood cells in a patient is provided. The method includes advancing to a location in an esophagus associated with eosinophilic esophagitis (EoE), a device provided with a collection portion having a first axial end portion and an inflatable second axial end portion. Thereafter, axially moving the second axial end portion, relative to the first axial end portion, from a collapsed position within the first axial end portion into an expanded position out from the first axial end portion. Once the second axial end portion is in the expanded position, allowing it to engage the location associated with EoE to collect a sample of cells.

公开(公告)号：US10660621B2

公开(公告)日：2020-05-26

申请号：US15103638

申请日：2014-12-12

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： Sanford Markowitz ,  Amitabh Chak ,  Joseph Willis ,  Jeffrey Silver Taggart ,  Rebecca Blice ,  Dean Secrest ,  Dennis Siedlak

IPC: A61B5/00 ,  A61B10/02

Abstract: A device for collecting a biological sample in a patient includes a collection portion having a first axial end portion and a second axial end portion. The second axial end portion has a collapsed position and an expanded position. The second axial end portion moves in an axial direction relative to the first axial end portion when the second axial end portion moves between the collapsed position and the expanded position. The second axial end portion extends axially into the first axial end portion and has a concave shape when in the collapsed position. The second axial end portion is convex when in the expanded position.

公开(公告)号：US20200077992A1

公开(公告)日：2020-03-12

申请号：US16610115

申请日：2018-05-03

Applicant: Case Western Reserve University

Inventor： Sanford Markowitz ,  Dean Secrest ,  Amitabh Chak ,  Joseph Willis ,  Dennis Siedlak

IPC: A61B10/04

Abstract: A device for collecting a biological sample in an esophagus of a patient includes a swallowable collection portion for collecting a sample at a collection site in the esophagus. A stylet is connected with the collection portion for placing the collection portion into the back of a throat of a patient for swallowing. The device may have a collection portion having a first axial end portion and a second axial end portion. A sleeve is in the first axial end portion. The device may have at least one tissue collecting projection extending from an outer surface of the collection portion; a first side wall of the tissue collecting; a second wall of the tissue collecting projection. A method for collecting a biological sample includes moving the swallowable collection portion with the stylet into the back of a throat of a patient for swallowing.

公开(公告)号：US20190390285A1

公开(公告)日：2019-12-26

申请号：US16564392

申请日：2019-09-09

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Joseph Willis ,  Amitabh Chak ,  Rom Leidner

IPC: C12Q1/6886 ,  C07H21/04

Abstract: This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as neoplasia of the upper or lower gastrointestinal tract, pancreas, and/or bladder.

公开(公告)号：US10465251B2

公开(公告)日：2019-11-05

申请号：US15568334

申请日：2016-04-20

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： Kishore Guda ,  Amitabh Chak ,  AnnMarie Kieber-Emmons

IPC: C12Q1/6886 ,  C12N15/11 ,  C12N15/113

Abstract: A method of analyzing tissue in a subject having or suspected of having cancer includes obtaining an expression profile from a sample of tissue obtained from the subject, wherein the expression profile comprises the level of at least one esophageal adenocarcinoma associated lincRNA selected from the group consisting of linc-PRKD, lincRTL, lincMIA, lincNAV, and lincTMEM.