公开(公告)号：US11104886B2

公开(公告)日：2021-08-31

申请号：US16467087

申请日：2017-12-04

Applicant: DSM IP Assets B.V.

Inventor： Johannes Gustaaf Ernst Van Leeuwen ,  Priscilla Zwartjens ,  Viktor Marius Boer

IPC: C12N9/02 ,  A23L27/30 ,  C12P19/12

Abstract: The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.

公开(公告)号：US10689681B2

公开(公告)日：2020-06-23

申请号：US14893216

申请日：2014-06-02

Applicant: DSM IP ASSETS B.V.

Inventor： Viktor Marius Boer ,  Erwin Suir

IPC: C12P19/56 ,  C12N9/88 ,  C12N9/02 ,  C12N9/16 ,  C12N15/52 ,  C12N9/90 ,  C07K14/39 ,  C12N9/10 ,  C12N9/24 ,  A23L27/30 ,  A23L2/60 ,  C07K14/395 ,  C12P15/00 ,  C07H15/256

Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.

公开(公告)号：US20150031868A1

公开(公告)日：2015-01-29

申请号：US14373571

申请日：2013-01-23

Applicant: DSM IP ASSETS B.V.

Inventor： Martin Lehmann ,  Joshua Trueheart ,  Priscilla Zwart Jens ,  Liang Wu ,  Viktor Marius Boer ,  Cornelis Maria Jacobus Sagt ,  Manoj Kumar ,  Bernard Meijrink ,  Marco Alexander Van Den Berg

IPC: C12P19/56 ,  C12N9/10 ,  A23L1/236 ,  C07H15/256

CPC classification number: C12P19/56 ,  A23K20/10 ,  A23K20/121 ,  A23K20/163 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256 ,  C12N9/0073 ,  C12N9/1051 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/007 ,  C12Y114/13078 ,  C12Y204/01035 ,  C12Y402/03019 ,  C12Y505/01013

Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.

Abstract translation: 本发明涉及包含一个或多个核苷酸序列的重组微生物，所述核苷酸序列编码：具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有海藻酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。 重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶，使得微生物能够产生一种或多种甜菊糖苷。