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公开(公告)号：US07473419B2
公开(公告)日：2009-01-06
申请号：US11268276
申请日：2005-11-07
CPC分类号： A61K48/0041 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K48/0083 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
摘要翻译： 公开了一种提供外源核酸在哺乳动物血管外实质细胞中表达的方法。 将核酸插入哺乳动物的容器中并增加血管的渗透性。 增加容器的渗透性允许将核酸递送至血管外实质细胞。
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22.发明授权Enhancing intermolecular integration of nucleic acids using integrator complexes 有权使用积分复合物增强核酸的分子间整合公开(公告)号：US07262056B2
公开(公告)日：2007-08-28
申请号：US10291342
申请日：2002-11-08
申请人： Christine Wooddell , Hans Herweijer , Jon A. Wolff
发明人： Christine Wooddell , Hans Herweijer , Jon A. Wolff
IPC分类号： C12N15/00 , C12N15/74 , C12N15/861 , C12N15/66 , C12N1/20
CPC分类号： C12N9/22 , C12N15/907 , C12N2800/90 , C12N2840/20
摘要： We disclose compositions and processes for enhancing transposon mediated integration of a nucleic acid molecule into another target nucleic acid molecule. Integration by an integrator complex is enhanced by cationic reagents.
摘要翻译： 我们公开了用于增强转座子介导的核酸分子整合到另一靶核酸分子中的组合物和方法。 通过阳离子试剂增强了积分器复合物的整合。
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公开(公告)号：US20070048254A1
公开(公告)日：2007-03-01
申请号：US11467033
申请日：2006-08-24
申请人： Zane Neal , Hans Herweijer
发明人： Zane Neal , Hans Herweijer
CPC分类号： A61K39/39 , A61K38/177 , A61K38/18 , A61K38/193 , A61K2039/545 , A61K2039/55522 , A61K2300/00
摘要： A method is provided for expanding the population of mature dendritic cells in an individual subject. Proper timing of sequential delivery of Flt3-L and GM-CSF provides improved expansion of mature dendritic cell populations over methods currently employed in the art. The expansion of mature dendritic cells can be used to enhance an immune response in the subject. The mature dendritic cells can also be isolated from the subject for use in biological research.
摘要翻译： 提供了一种用于扩大个体受试者中成熟树突细胞群体的方法。 顺序递送Flt3-L和GM-CSF的适当时机提高了成熟树突状细胞群体与本领域目前使用的方法的扩增。 成熟树突状细胞的扩增可用于增强受试者的免疫应答。 成熟树突状细胞也可以从受试者中分离出来用于生物研究。
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公开(公告)号：US07015040B2
公开(公告)日：2006-03-21
申请号：US11008856
申请日：2004-12-10
IPC分类号： C12N15/88
CPC分类号： A61K48/0041 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K48/0083 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
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公开(公告)号：US06867196B1
公开(公告)日：2005-03-15
申请号：US09330909
申请日：1999-06-11
申请人： Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人： Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
CPC分类号： A61K48/0008 , A61K48/0075
摘要： A process for delivering a nucleic acid to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a nucleic acid to a blood vessel, which subsequently delivers the nucleic acid to the cardiac tissue cell. The nucleic acid can be DNA or RNA or plasmid DNA or viral. This process is for purposes of gene therapy, and research.
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公开(公告)号：US07781415B2
公开(公告)日：2010-08-24
申请号：US10770588
申请日：2004-02-03
申请人： Hans Herweijer , Julia Hegge , Jon A. Wolff , David L. Lewis
发明人： Hans Herweijer , Julia Hegge , Jon A. Wolff , David L. Lewis
IPC分类号： A61K48/00
CPC分类号： A61K48/0083
摘要： A process for delivering a polynucleotide to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a polynucleotide into a blood vessel and increasing permeability of the blood vessel to the polynucleotide. The polynucleotide can be a small interfering RNA or microRNA and inhibit gene expression in the cell.
摘要翻译： 描述了将多核苷酸递送至哺乳动物心脏组织细胞的方法，包括将由多核苷酸组成的组合物引入血管并增加血管对多核苷酸的通透性。 多核苷酸可以是小干扰RNA或微小RNA并抑制细胞中的基因表达。
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公开(公告)号：US20100120152A1
公开(公告)日：2010-05-13
申请号：US10229786
申请日：2002-08-27
CPC分类号： C12N15/85 , C07K14/765 , C12N2830/30 , C12N2830/34 , C12N2830/42 , C12N2830/50 , C12N2830/85
摘要： The present invention relates to compositions and methods for increasing long term expression in vitro and in vivo, comprising sequences and gene expression cassettes that increase expression of genes to which they are operably linked.
摘要翻译： 本发明涉及用于在体外和体内增加长期表达的组合物和方法，其包含增加其可操作连接的基因表达的序列和基因表达盒。
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公开(公告)号：US07589059B2
公开(公告)日：2009-09-15
申请号：US10628792
申请日：2003-07-28
申请人： Jon A. Wolff , Vladimir G. Budker , Hans Herweijer , James E. Hagstrom , Sean D. Monahan , Julia Hegge , Vladimir Subbotin
发明人： Jon A. Wolff , Vladimir G. Budker , Hans Herweijer , James E. Hagstrom , Sean D. Monahan , Julia Hegge , Vladimir Subbotin
CPC分类号： A61K9/0019 , A61K38/00 , A61K48/0075 , C12N15/87 , C12N2799/022
摘要： Disclosed is a system for providing in vivo delivery of molecules or complexes to extravascular mammalian cells using an intravascular administration route. The molecules or complexes are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate transiently increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the molecule to the cell.
摘要翻译： 公开了一种使用血管内施用途径向血管外哺乳动物细胞体内递送分子或复合物的系统。 将分子或复合物插入哺乳动物脉管系统的注射溶液中。 以适当的速率插入注射溶液瞬时增加组织中血管外流体的体积，从而便于将分子递送至细胞。
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公开(公告)号：US06458382B1
公开(公告)日：2002-10-01
申请号：US09709656
申请日：2000-11-10
申请人： Hans Herweijer , Vladimir G. Budker
发明人： Hans Herweijer , Vladimir G. Budker
IPC分类号： A61K9127
CPC分类号： C12N15/88 , A61K9/1272
摘要： The present invention relates to compositions and methods for transferring nucleic acids into cells in vitro and in vivo. The compositions comprise a transfection reagent and one or more detergents. In preferred embodiments, the compositions comprise delivery systems providing nucleic acid transfer complexes that transfect cells with high efficiency.
摘要翻译： 本发明涉及在体外和体内将核酸转运到细胞中的组合物和方法。 组合物包含转染试剂和一种或多种洗涤剂。 在优选实施方案中，组合物包含提供以高效转染细胞的核酸转移复合物的递送系统。
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