公开(公告)号：US10781455B2

公开(公告)日：2020-09-22

申请号：US15612024

申请日：2017-06-02

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Andrew F. Worden ,  Stephen Novak ,  Ryan M. Lee

IPC: C12N15/82

Abstract: The current disclosure relates to methods and compositions for improving plant varieties through plant breeding and plant genetics. For instance, the disclosure concerns increasing the recombination frequency of a heterozygous trait genetically linked to a second trait within plants. Further, the disclosure concerns breaking the genetic linkage between a first allele and a second allele.

公开(公告)号：US10443067B2

公开(公告)日：2019-10-15

申请号：US15835595

申请日：2017-12-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Daren Hemingway ,  Carla Ausmus ,  Andrew F. Worden ,  Andrew Asberry

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Brachypodium distachyon metallothionein-like gene (mtl). Some embodiments relate to a promoter from a Brachypodium distachyon metallothionein-like gene (mtl) that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US10273494B2

公开(公告)日：2019-04-30

申请号：US15484581

申请日：2017-04-11

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC: C12N15/82 ,  C12N15/00

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US20190100764A1

公开(公告)日：2019-04-04

申请号：US16139112

申请日：2018-09-24

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Sara Bennett ,  Andrew J. Bowling ,  Alicia Walker ,  Cory M. Larsen ,  Heather Pence ,  Staci Weaver ,  Ronnie Hampton, JR.

IPC: C12N15/82

Abstract: This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Glycine max egg cell gene. Some embodiments relate to a promoter or a 5′ UTR from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

公开(公告)号：US10036028B2

公开(公告)日：2018-07-31

申请号：US14587757

申请日：2014-12-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/82

CPC classification number: C12N15/8216

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v1.

公开(公告)号：US10030246B2

公开(公告)日：2018-07-24

申请号：US14587735

申请日：2014-12-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/82 ,  C12N15/10

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. B104.

公开(公告)号：US20180142249A1

公开(公告)日：2018-05-24

申请号：US15797285

申请日：2017-10-30

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Joseph F. Petolino ,  Andrew F. Worden ,  Pierluigi Barone ,  Matthew A. Simpson ,  Tonya L. Strange

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C07K2319/81 ,  C12N15/8201 ,  C12N15/8209 ,  C12N15/8222 ,  C12N15/8231 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8261 ,  C12N15/8274 ,  C12N15/8286

Abstract: Compositions and methods to modify at least one target locus in a plant cell are provided, which comprises providing a plant cell, a plant, or a plant part with one or more target loci and one or more donor loci, providing at least one cleaving site specific nuclease to produce a double strand break within the target loci, followed by non-homologous end joining of at least one donor locus within at least one target locus. Target loci, donor loci and nuclease loci used in these methods, and plant cells, plants and plant parts comprising these target loci, donor loci, nuclease loci and/or the recombined loci are also provided.

公开(公告)号：US09885054B2

公开(公告)日：2018-02-06

申请号：US15618986

申请日：2017-06-09

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/10 ,  C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/10

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.

公开(公告)号：US09765350B2

公开(公告)日：2017-09-19

申请号：US14844731

申请日：2015-09-03

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sara Bennett ,  Sandeep Kumar ,  Donald Merlo ,  Nagesh Sardesai

IPC: C12N15/82 ,  C12N15/74

CPC classification number: C12N15/8205 ,  C12N15/743

Abstract: The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.

公开(公告)号：US20170137834A1

公开(公告)日：2017-05-18

申请号：US15331126

申请日：2016-10-21

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sandeep Kumar ,  Navin Elango ,  Jeffrey Beringer ,  Shavell Gorman ,  Andrew F. Worden ,  Sara Bennett ,  Daren Hemingway ,  Wei Chen ,  Huixia Wu ,  Ning Zhou ,  Michelle Sprint Smith

IPC: C12N15/82 ,  C07K14/325

CPC classification number: C12N15/8227 ,  C12N9/14 ,  C12N15/8225 ,  C12N15/8286 ,  C12Y303/01001 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays GRMZM2G015295 gene regulatory elements.