公开(公告)号：US11149287B2

公开(公告)日：2021-10-19

申请号：US16246864

申请日：2019-01-14

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  C12N15/90

Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10961540B2

公开(公告)日：2021-03-30

申请号：US16698077

申请日：2019-11-27

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/63 ,  C12N15/79

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US10415046B2

公开(公告)日：2019-09-17

申请号：US15338888

申请日：2016-10-31

Applicant: DOW AGROSCIENCES LLC

Inventor： W. Michael Ainley ,  James W. Bing ,  David R. Corbin ,  Steven L. Evans ,  Joseph F. Petolino ,  Lakshmi Sastry-Dent ,  Steven A. Thompson ,  Steven R. Webb ,  Mary E. Welter ,  Ning Zhou

IPC: C12N15/82 ,  C12N9/12

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

公开(公告)号：US10233465B2

公开(公告)日：2019-03-19

申请号：US14531732

申请日：2014-11-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  C12N15/90

Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10106804B2

公开(公告)日：2018-10-23

申请号：US15867770

申请日：2018-01-11

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper ,  W. Michael Ainley

IPC: C12N15/82 ,  A01H5/10

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US20180223297A1

公开(公告)日：2018-08-09

申请号：US15943106

申请日：2018-04-02

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C07K14/47 ,  C12N9/22 ,  C12N15/90 ,  C12N15/66 ,  C12N15/10

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US09963711B2

公开(公告)日：2018-05-08

申请号：US14019244

申请日：2013-09-05

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C12N15/66 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90 ,  C07K14/47

CPC classification number: C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US09914930B2

公开(公告)日：2018-03-13

申请号：US14019211

申请日：2013-09-05

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/79 ,  C12N15/63

CPC classification number: C12N15/79 ,  C12N9/22 ,  C12N15/63 ,  C12N15/8241 ,  C12N15/8247

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US09719108B2

公开(公告)日：2017-08-01

申请号：US14861864

申请日：2015-09-22

Applicant: Dow AgroSciences LLC

Inventor： Jayakumar Pon Samuel ,  Joseph F. Petolino ,  Narasimha Chary Samboju ,  Steven R. Webb ,  Kerrm Y. Yau

IPC: C12N15/90 ,  C12N15/82 ,  C12N5/04 ,  C12N9/22 ,  C12N9/96

CPC classification number: C12N15/902 ,  C12N5/04 ,  C12N9/22 ,  C12N9/96 ,  C12N15/8206 ,  C12Y301/21004

Abstract: Provided are methods for introducing a sequence-specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

公开(公告)号：US20140090112A1

公开(公告)日：2014-03-27

申请号：US14019244

申请日：2013-09-05

Applicant: Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

Abstract translation: FAD2基因座中基因编辑或基因堆积的方法，以位点方式，以细胞中的FAD2基因的位置切割，在FAD2基因中产生断裂，然后连接到断裂中，与核酸分子缔合 公开了一种或多种感兴趣的特征。