公开(公告)号：US20180113295A1

公开(公告)日：2018-04-26

申请号：US15850969

申请日：2017-12-21

Applicant: FUJIFILM Corporation

Inventor： Kenta MATSUBARA

IPC: G02B21/14 ,  G02B21/26 ,  G02B7/28 ,  G02B21/00

CPC classification number: G02B21/14 ,  G02B7/28 ,  G02B7/282 ,  G02B7/36 ,  G02B21/0004 ,  G02B21/06 ,  G02B21/26

Abstract: A culture vessel that houses a culture liquid and a specimen is irradiated with pattern light having a pattern that is previously set for the culture vessel. Transmitted light transmitted through the culture liquid in the culture vessel because of the irradiation with the pattern light is detected. Optical characteristics of an adjusting optical system that adjusts refraction of light caused by the shape of the liquid surface of the culture liquid in the culture vessel are adjusted, on the basis of a detection signal based on the detected transmitted light. After the adjustment, the culture vessel is irradiated with illumination light for phase-contrast measurement, and the specimen irradiated with the illumination light is imaged.

公开(公告)号：US20170322405A1

公开(公告)日：2017-11-09

申请号：US15601117

申请日：2017-05-22

Applicant: FUJIFILM Corporation

Inventor： Kenta MATSUBARA

IPC: G02B21/14 ,  G02B21/36 ,  G02B21/02

CPC classification number: G02B21/14 ,  G02B3/14 ,  G02B21/025 ,  G02B21/086 ,  G02B21/36 ,  G02B21/361 ,  H04N5/2256 ,  H04N5/23212 ,  H04N5/2354

Abstract: The phase contrast microscope includes: an illumination optical system 10 that emits illumination light for phase difference measurement to an observation target placed in a container; an adjustment optical system 20 that is provided between the illumination optical system 10 and the observation target S, has at least one optical element 21, and adjusts refraction of the illumination light due to a liquid surface shape of a liquid C in the container 60; an imaging unit 40 that images the observation target to which the illumination light has been emitted; and an adjustment optical system control unit 51 that adjusts optical characteristics of the adjustment optical system 20 based on uniformity of a density of an image captured by the imaging unit 40 and a density contrast.

公开(公告)号：US20170146558A1

公开(公告)日：2017-05-25

申请号：US15424302

申请日：2017-02-03

Applicant: FUJIFILM Corporation

Inventor： Yasuyuki ISHII ,  Kenta MATSUBARA ,  Shinichiro FUKUI

IPC: G01N33/80 ,  C12Q1/68 ,  G01N21/31

CPC classification number: G01N33/80 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/6883 ,  G01N21/27 ,  G01N21/31 ,  G01N33/48 ,  G01N33/483 ,  G01N33/49

Abstract: A method for sorting nucleated red blood cells, the method includes: selecting a candidate cell of nucleated red blood cells in accordance with shapes of cells; setting a search range including the candidate cell and selecting a white blood cell within the search range; defining that the candidate cell is a nucleated red blood cell through comparison of spectral characteristics of hemoglobin between the candidate cell and the white blood cell; and sorting the nucleated red blood cells into mother-derived nucleated red blood cells or fetus-derived nucleated red blood cells through comparison of an allele contained in the nucleated red blood cell after the defining of the nucleated red blood cell with an allele contained in a white blood cell which has not been selected in the selecting of the candidate cell or in the white blood cell selected in the selecting of the white blood cell.

公开(公告)号：US20170145512A1

公开(公告)日：2017-05-25

申请号：US15423998

申请日：2017-02-03

Applicant: FUJIFILM Corporation

Inventor： Shinichiro FUKUI ,  Yasuyuki ISHII ,  Kenta MATSUBARA

IPC: C12Q1/68 ,  G01N21/31 ,  G01N33/49

CPC classification number: C12Q1/6883 ,  C12Q1/04 ,  C12Q1/68 ,  G01N1/4077 ,  G01N15/1475 ,  G01N21/27 ,  G01N21/31 ,  G01N33/48 ,  G01N33/49 ,  G01N2001/4083 ,  G01N2015/0073 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/3133

Abstract: A method for inspecting a chromosome of a fetus includes: a collection step of obtaining maternal blood from a pregnant mother; a concentration step of concentrating nucleated red blood cells in the maternal blood; a sorting step of sorting the nucleated red blood cells in the maternal blood, in which the nucleated red blood cells are concentrated through the concentration step, into mother-derived nucleated red blood cells and fetus-derived nucleated red blood cells in accordance with the shapes of nuclei and spectral characteristics at wavelengths included in a light wavelength region of 400 to 650 nm; an amplification step of amplifying a nucleic acid of a chromosome of the nucleated red blood cells; a definition step of defining an amount of the amplified product of the nucleated red blood cells; and a determination step of determining presence or absence of a numerical abnormality of the fetus-derived chromosome.

公开(公告)号：US20170061618A1

公开(公告)日：2017-03-02

申请号：US15348008

申请日：2016-11-10

Applicant: FUJIFILM Corporation

Inventor： Kenta MATSUBARA

IPC: G06T7/00 ,  C12Q1/02

CPC classification number: G06T7/0012 ,  C12M41/36 ,  C12Q1/02 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The present invention provides a cell evaluation device, a cell evaluation method and a non-transitory computer readable recording medium storing a cell evaluation program capable of evaluating an evaluation target cell. The cell evaluation device includes an image acquisition unit that acquires a cell image obtained by imaging a cell group; a cell evaluation unit that specifies an evaluation target cell and peripheral cells around the evaluation target cell in the cell group, and evaluates the evaluation target cell based on evaluation results of the peripheral cells; and a boundary setting unit that sets a boundary in the cell image based on a state of the cell group, in which when specifying the peripheral cells, the cell evaluation unit specifies only cells that are present in a divided region where the evaluation target cell is present among plural divided regions divided by the boundary, as the peripheral cells.

Abstract translation: 本发明提供了一种存储能够评估评估对象单元的小区评价程序的小区评价装置，小区评价方法和非暂时性计算机可读记录介质。 电池评估装置包括：图像获取单元，其获取通过对单元组成像获得的单元图像; 细胞评估单元，其指定细胞组中的评价对象细胞周围的评价对象细胞和周边细胞，并根据周边细胞的评价结果​​评价评价对象细胞; 以及边界设定单元，其基于小区组的状态来设定小区图像中的边界，其中当指定周边小区时，小区评估单元仅指定在评估目标小区为分割区域中存在的小区 作为周边细胞存在于由边界划分的多个分割区域中。

公开(公告)号：US20170011512A1

公开(公告)日：2017-01-12

申请号：US15273789

申请日：2016-09-23

Applicant: FUJIFILM Corporation

Inventor： Kenta MATSUBARA

IPC: G06T7/00 ,  G06T11/60 ,  G06T7/40

CPC classification number: G06T7/0012 ,  G06K9/00147 ,  G06T11/60 ,  G06T2207/30024

Abstract: A nucleolus detection unit, which detects nucleoli in a plurality of cells in a cell image obtained by imaging the cells, and a cell recognition unit, which acquires information indicating a distance between the nucleoli and recognizes the individual cells based on the information indicating the distance, are provided.

Abstract translation: 核细胞检测单元，其检测通过对细胞成像获得的细胞图像中的多个细胞中的核仁;以及细胞识别单元，其基于指示距离的信息获取指示核仁之间的距离并识别各个细胞的信息 ，提供。

公开(公告)号：US20160366318A1

公开(公告)日：2016-12-15

申请号：US15248262

申请日：2016-08-26

Applicant: FUJIFILM Corporation

Inventor： Kenta MATSUBARA

IPC: H04N5/225 ,  G02B21/24 ,  G02B21/14

CPC classification number: H04N5/2256 ,  G02B21/14 ,  G02B21/18 ,  G02B21/244 ,  G02B21/245 ,  G02B21/248

Abstract: Provided is an observation device that appropriately illuminates a subject with structured illumination light. The device is provided with: a structured illumination section; a phase difference measurement illumination section; a phase contrast lens that has a phase plate for dimming illumination light for phase difference measurement, where the illumination light for phase difference measurement is incident into the lens, and the structured illumination light is incident into the lens from a side opposite to an incidence side of the ring-shaped illumination; a detection section that detects reflected light of the structured illumination light; and an observation section that images the illumination light for phase difference measurement. When Fourier transform is performed on the structured illumination light, a spatial frequency of the structured illumination light is set on a high-frequency side or a low-frequency side with respect to a position of the phase plate 32 on optical Fourier space.

Abstract translation: 提供了一种利用结构化的照明光适当地照射被摄体的观察装置。 该装置具有：结构化照明部分; 相位差测量照明部; 相位差透镜，其具有用于调光相位差测量的照明光的相位板，其中用于相位差测量的照明光入射到透镜中，并且结构化照明光从入射侧相反的一侧入射到透镜中 的环形照明; 检测部，其检测所述结构化照明光的反射光; 以及对相位差测量的照明光进行成像的观察部。 当对结构照明光进行傅里叶变换时，相对于相位板32在光学傅立叶空间上的位置，在高频侧或低频侧设置结构化照明光的空间频率。

公开(公告)号：US20160161394A1

公开(公告)日：2016-06-09

申请号：US15045691

申请日：2016-02-17

Applicant: FUJIFILM Corporation

Inventor： Kenta MATSUBARA

IPC: G01N15/14 ,  G06T7/20 ,  H04N5/232 ,  H04N5/235 ,  G06T7/00 ,  G06K9/62

CPC classification number: G01N15/1463 ,  C12M41/36 ,  C12Q1/04 ,  G01N15/1475 ,  G01N2015/0088 ,  G01N2015/1006 ,  G01N2015/1488 ,  G01N2015/1493 ,  G01N2015/1497 ,  G06K9/00134 ,  G06K9/00147 ,  G06K9/6201 ,  G06K2009/6213 ,  G06T7/0012 ,  G06T7/20 ,  G06T2207/30024 ,  H04N5/23296 ,  H04N5/2353

Abstract: An observation image determination device includes an observation image acquisition unit that captures an image of an observation region including a stem cell to be cultured to acquire an observation image and a determination unit that determines whether a living body of a different type from the stem cell is included in the observation region. The determination unit determines whether the different type of living body is included in the observation region, on the basis of at least one of form information of an observation target and information about a change in the observation target over time which are acquired from the observation image.

Abstract translation: 观察图像确定装置包括观察图像获取单元，其捕获包括待培养的干细胞的观察区域的图像以获取观察图像;以及确定单元，其确定与干细胞不同类型的生物体是否为 包括在观察区域。 判断单元根据从观察图像获取的观察对象的形态信息和关于观察对象随时间的变化的信息中的至少一个，判定观察区域中是否包含不同类型的生物体 。