公开(公告)号：US08604184B2

公开(公告)日：2013-12-10

申请号：US12774194

申请日：2010-05-05

申请人： Kary B. Mullis ,  Jeevalatha Vivekananda ,  Johnathan Lloyd Kiel ,  Ronald M. Cook

发明人： Kary B. Mullis ,  Jeevalatha Vivekananda ,  Johnathan Lloyd Kiel ,  Ronald M. Cook

IPC分类号： A61K48/00 ,  C07H21/04

CPC分类号： A61K31/7088 ,  Y02A50/471 ,  Y02A50/473

摘要： Methods and compositions for immediately immunizing an individual against any molecule or compound are provided. The present invention is directed to an immunity linker with at least two sites; (1) at least one first binding site that binds to an immune response component in an individual, and (2) at least one second binding site that binds specifically to a desired compound or molecule, the target. The second binding sites are preferably thiolated aptamers that have the benefit of increased stability, resistance to degradation and longer circulating half life. Methods of making and using pharmaceutical compositions including immunity linker molecules having a thiolated aptamer are also provided.

摘要翻译： 提供了立即将个体免疫任何分子或化合物的方法和组合物。 本发明涉及具有至少两个位点的免疫接头; （1）结合个体中的免疫应答组分的至少一个第一结合位点，和（2）至少一个第二结合位点，其特异性结合所需化合物或分子，靶标。 第二结合位点优选为具有增加的稳定性，耐降解性和更长的循环半衰期益处的硫醇化适体。 还提供了制备和使用药物组合物的方法，包括具有巯基适体的免疫接头分子。

公开(公告)号：US20120276136A1

公开(公告)日：2012-11-01

申请号：US13543718

申请日：2012-07-06

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： A61K39/00 ,  A61K39/12 ,  C07K9/00 ,  C07H21/04 ,  A61K39/385 ,  C07K14/00 ,  A61K39/02 ,  C07H21/02

CPC分类号： A61K39/385 ,  A61K2039/6025 ,  Y02A50/386

摘要： The present invention is related to methods and compositions that are capable of immediately immunizing a human or animal against any molecule or compound. The present invention comprises an immunity linker molecule with at least two sites; (1) a first binding site that binds to an immune system molecule in a human or animal that has been preimmunized against the first binding site, and (2) one or more second binding sites that bind specifically to a desired compound or molecule. The first binding site and the second binding site(s) are linked by a linker portion of the molecule.

摘要翻译： 本发明涉及能够立即针对任何分子或化合物免疫人或动物的方法和组合物。 本发明包括具有至少两个位点的免疫接头分子; （1）结合已针对第一结合位点预免疫的人或动物中的免疫系统分子的第一结合位点，和（2）特异性结合所需化合物或分子的一个或多个第二结合位点。 第一结合位点和第二结合位点通过分子的接头部分连接。

公开(公告)号：US20100247535A1

公开(公告)日：2010-09-30

申请号：US12685257

申请日：2010-01-11

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： A61K39/395 ,  A61P31/00 ,  A61K35/76 ,  A61K35/74

CPC分类号： A61K39/07 ,  A61K39/385 ,  A61K2039/505 ,  A61K2039/541 ,  A61K2039/6025 ,  A61K2039/627 ,  C07K16/1242 ,  C07K2317/55 ,  Y02A50/386

摘要： Methods and compositions for immediately immunizing an individual against any molecule or compound. The present invention comprises an immunity linker with at least two sites; (1) at least one first binding site that binds to an immune response component in an individual that has been pre-immunized with a universal immunogen, and (2) at least one second binding site that binds specifically to a desired compound or molecule, the target.

摘要翻译： 立即将个体免疫任何分子或化合物的方法和组合物。 本发明包括具有至少两个位点的免疫接头; （1）至少一个第一结合位点，其结合已经用通用免疫原预先免疫的个体中的免疫应答组分，和（2）至少一个特异性结合所需化合物或分子的第二结合位点， 目标。

公开(公告)号：US07645743B2

公开(公告)日：2010-01-12

申请号：US10754456

申请日：2004-01-09

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： A61K31/505 ,  C07H21/00

CPC分类号： A61K39/07 ,  A61K39/385 ,  A61K2039/505 ,  A61K2039/541 ,  A61K2039/6025 ,  A61K2039/627 ,  C07K16/1242 ,  C07K2317/55 ,  Y02A50/386

摘要： Methods and compositions for immediately immunizing an individual against any molecule or compound. The present invention comprises an immunity linker with at least two sites; (1) at least one first binding site that binds to an immune response component in an individual that has been pre-immunized with a universal immunogen, and (2) at least one second binding site that binds specifically to a desired compound or molecule, the target.

摘要翻译： 立即将个体免疫任何分子或化合物的方法和组合物。 本发明包括具有至少两个位点的免疫接头; （1）至少一个第一结合位点，其结合已经用通用免疫原预先免疫的个体中的免疫应答组分，和（2）至少一个特异性结合所需化合物或分子的第二结合位点， 目标。

公开(公告)号：US5656493A

公开(公告)日：1997-08-12

申请号：US199505

申请日：1994-02-18

申请人： Kary B. Mullis ,  Larry Johnson ,  Richard A. Leath ,  Timothy J. Wennberg ,  Louis M. Mezei ,  Joseph T. Widunas

发明人： Kary B. Mullis ,  Larry Johnson ,  Richard A. Leath ,  Timothy J. Wennberg ,  Louis M. Mezei ,  Joseph T. Widunas

IPC分类号： B01J19/00 ,  B01L7/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  C12M3/02 ,  C12M1/00 ,  C12N15/00 ,  G01N21/00

CPC分类号： B01L7/52 ,  B01J19/0046 ,  C12Q1/686 ,  B01J2219/00495 ,  B01J2219/0059 ,  B01J2219/00689 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

摘要： There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.

摘要翻译： 本文公开了一种用于在计算机控制下进行核酸扩增的机器。 该机器在主机的控制下使用多个加热和冷却系统中的任何一个，其引导加热和冷却系统在该过程中适当的时间对含反应室的热交换器进行加热和冷却。 反应室预先加载待扩增的核酸，催化聚合的热稳定酶，特异性寡核苷酸引物和四种不同的核苷酸三磷酸。 还公开了由机器实现的扩增链反应的方法，其利用热稳定性酶。

公开(公告)号：US5028792A

公开(公告)日：1991-07-02

申请号：US344274

申请日：1989-04-27

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： G01J1/50 ,  G03C1/73

CPC分类号： G01J1/50 ,  G03C1/73

摘要： Photochemical systems for the direct visualization of exposure to ultraviolet radiation that effect visible color changes involving a process in which a photoacid is formed upon irradiation of a nitro-substituted aromatic aldehyde with ultraviolet light and wherein proton transfer to a dye causes the dye to undergo a visible color change. The system undergoes such color change to an extent directly proportional to the cumulative amount of ultraviolet incident thereupon.

摘要翻译： 用于直接观察紫外线照射的光化学体系，其影响可见颜色变化，其涉及在用紫外线照射硝基取代的芳族醛时形成光致酸的方法，并且其中质子转移到染料使染料经历 可见颜色变化。 该系统经历与其入射的紫外线累积量成正比的变化。

公开(公告)号：US4803297A

公开(公告)日：1989-02-07

申请号：US72339

申请日：1987-07-13

申请人： Corey H. Levenson ,  Kary B. Mullis

发明人： Corey H. Levenson ,  Kary B. Mullis

IPC分类号： C07D493/04 ,  C07D513/04 ,  C07D519/00 ,  C07H21/00 ,  C07C125/06

CPC分类号： C07D493/04 ,  C07D513/04 ,  C07H21/00

摘要： Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:[A--[B--Lwhere A is an alkylating intercalation moiety, B is a divalent organic moiety of the formula: ##STR1## where Y is O, NH or N--CHO, x is a number from 1 to 4, y is a number from 2 to 4, and L is a monovalent label moiety, wherein B is exclusive of any portion of the intercalation and label moieties.Preferably A is a 4-methylene-substituted psoralen moiety, and most preferably A is a 4'-methylene-substituted-4,5',8-trimethylpsoralen moiety and L is biotin.

摘要翻译： 可以通过将标记试剂的烷基化插层部分嵌入部分双链核酸中以形成复合物并激活复合物以引起试剂和核酸之间的共价键来标记核酸。 优选地，标记的核酸是用于检测能够与核酸的杂交区杂交的核酸序列的杂交探针。 还优选地，标记部分是非放射性的。 标记试剂具有下式：[A- [BL，其中A是烷基化插层部分，B是下式的二价有机部分：其中Y是O，NH或N-CHO，x是从 1至4，y为2至4的数，L为单价标记部分，其中B不包括插层和标记部分的任何部分。 优选A是4-亚甲基取代的补骨脂素部分，最优选A是4'-亚甲基取代的-4,5'，8-三甲基肉豆蔻酸酯部分，L是生物素。

公开(公告)号：US4751313A

公开(公告)日：1988-06-14

申请号：US72531

申请日：1987-07-13

申请人： Corey H. Levenson ,  Kary B. Mullis

发明人： Corey H. Levenson ,  Kary B. Mullis

IPC分类号： C07D493/04 ,  C07D513/04 ,  C07D519/00 ,  C07H21/00 ,  C07D495/04

CPC分类号： C07D493/04 ,  C07D513/04 ,  C07H21/00

摘要： Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:[A][B]Lwhere A is an alkylating intercalation moiety, B is a divalent organic moiety of the formula: ##STR1## where Y is O, NH or N--CHO, x is a number from 1 to 4, y is a number from 2 to 4, and L is a monovalent label moiety, wherein B is exclusive of any portion of the intercalation and label moieties.Preferably A is a 4-methylene-substituted psoralen moiety, and most preferably A is a 4'-methylene-substituted-4,5',8-trimethylpsoralen moeity and L is biotin.

摘要翻译： 可以通过将标记试剂的烷基化插层部分嵌入部分双链核酸中以形成复合物并激活复合物以引起试剂和核酸之间的共价键来标记核酸。 优选地，标记的核酸是用于检测能够与核酸的杂交区域杂交的核酸序列的杂交探针。 还优选地，标记部分是非放射性的。 标记试剂具有下式：[A] [B] L，其中A是烷基化插层部分，B是下式的二价有机部分：其中Y是O，NH或N-CHO，x是 1至4的数，y为2至4的数，L为单价标记部分，其中B不包括插入和标记部分的任何部分。 优选A是4-亚甲基取代的补骨脂素部分，最优选A是4'-亚甲基取代的-4,5'，8-三甲基肉桂酸酯，L是生物素。

公开(公告)号：US08591910B2

公开(公告)日：2013-11-26

申请号：US13543718

申请日：2012-07-06

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： A61K38/14 ,  A61K39/00 ,  A61K39/385 ,  C07K9/00 ,  C07H21/04 ,  C12N15/115

CPC分类号： A61K39/385 ,  A61K2039/6025 ,  Y02A50/386

摘要： The present invention is related to methods and compositions that are capable of immediately immunizing a human or animal against any molecule or compound. The present invention comprises an immunity linker molecule with at least two sites; (1) a first binding site that binds to an immune system molecule in a human or animal that has been preimmunized against the first binding site, and (2) one or more second binding sites that bind specifically to a desired compound or molecule. The first binding site and the second binding site(s) are linked by a linker portion of the molecule.

公开(公告)号：US20090142368A1

公开(公告)日：2009-06-04

申请号：US12336746

申请日：2008-12-17

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： A61K39/00

CPC分类号： A61K39/385 ,  A61K2039/6025 ,  Y02A50/386

摘要： The present invention is related to methods and compositions that are capable of immediately immunizing a human or animal against any molecule or compound. The present invention comprises an immunity linker molecule with at least two sites; (1) a first binding site that binds to an immune system molecule in a human or animal that has been preimmunized against the first binding site, and (2) one or more second binding sites that bind specifically to a desired compound or molecule. The first binding site and the second binding site(s) are linked by a linker portion of the molecule.

摘要翻译： 本发明涉及能够立即针对任何分子或化合物免疫人或动物的方法和组合物。 本发明包括具有至少两个位点的免疫接头分子; （1）结合已针对第一结合位点预免疫的人或动物中的免疫系统分子的第一结合位点，和（2）特异性结合所需化合物或分子的一个或多个第二结合位点。 第一结合位点和第二结合位点通过分子的接头部分连接。