公开(公告)号：US5468613A

公开(公告)日：1995-11-21

申请号：US491210

申请日：1990-03-09

申请人： Henry A. Erlich ,  Glenn Horn ,  Randall K. Saiki ,  Kary B. Mullis

发明人： Henry A. Erlich ,  Glenn Horn ,  Randall K. Saiki ,  Kary B. Mullis

IPC分类号： C12N15/09 ,  C07K14/74 ,  C12Q1/68 ,  G01N33/50 ,  C12N9/12 ,  C12N15/11 ,  C12P19/34

CPC分类号： C12Q1/6837 ,  C07K14/70539 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/156

摘要： Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.

摘要翻译： 核酸序列中的单个或多个核苷酸变异可通过以下过程在核酸中检测，其中怀疑含有相关核酸的样品用引物，三磷酸核苷酸和用于聚合三磷酸酯的试剂重复处理，然后变性 如果存在，则扩增含有核苷酸变异的序列的过程。 在一个实施方案中，将样品点在膜上并用标记的序列特异性寡核苷酸探针处理。 通过探头上的标签检测探针与样品的杂交。

公开(公告)号：US5187083A

公开(公告)日：1993-02-16

申请号：US611921

申请日：1990-11-13

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： C12N1/06 ,  C12N15/10

CPC分类号： C12N1/06 ,  C12N15/1017 ,  Y10S435/803 ,  Y10S435/82

摘要： The present invention provides a method for rapidly obtaining substantially pure DNA from a biological sample containing cells. The method involves gently lysing the membranes of the cells to yield a lysate containing genomic DNA in a high molecular weight form. The lysate is moved through a porous filter to selectively trap the high molecular weight DNA on the filter. The DNA is released from the filter using an aqueous solution to form a solution containing substantially purified DNA, from which the DNA is recovered.

摘要翻译： 本发明提供从含有细胞的生物样品中快速获得基本上纯的DNA的方法。 该方法包括轻轻地裂解细胞的膜以产生含有高分子量形式的基因组DNA的裂解物。 将裂解物移动通过多孔过滤器以选择性地将高分子量DNA捕获在过滤器上。 使用水溶液从过滤器中释放DNA以形成含有基本上纯化的DNA的溶液，从中回收DNA。

公开(公告)号：US4965188A

公开(公告)日：1990-10-23

申请号：US63647

申请日：1987-06-17

申请人： Kary B. Mullis ,  Henry A. Erlich ,  David H. Gelfand ,  Glenn Horn ,  Randall K. Saiki

发明人： Kary B. Mullis ,  Henry A. Erlich ,  David H. Gelfand ,  Glenn Horn ,  Randall K. Saiki

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： C12P19/34 ,  C12N9/1252 ,  C12Q1/6844

摘要： A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The amplified sequence can be readily detected. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

摘要翻译： 用于扩增核酸或其混合物中包含的任何靶核酸序列的方法包括用摩尔过量的两个寡核苷酸引物处理所述核酸的分开的互补链，并用热稳定酶扩增引物以形成互补引物延伸产物，其起作用 作为合成所需核酸序列的模板。 可以容易地检测扩增的序列。 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。

公开(公告)号：US4683202A

公开(公告)日：1987-07-28

申请号：US791308

申请日：1985-10-25

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： C12N15/09 ,  B01L7/00 ,  C07H21/00 ,  C07K14/805 ,  C12N15/00 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  C07H21/02 ,  C07H21/04 ,  C12N1/00

CPC分类号： C12N15/10 ,  B01L7/52 ,  C07K14/805 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  B01J2219/00495 ,  B01J2219/0059 ,  B01J2219/00722 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q1/689 ,  C40B40/06 ,  C40B60/14

摘要： The present invention is directed to a process for amplifying any desired specific nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, and extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.

公开(公告)号：US08236321B2

公开(公告)日：2012-08-07

申请号：US12336746

申请日：2008-12-17

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： A61K39/00 ,  A61K31/7088 ,  A61K31/7125 ,  A61K31/713

CPC分类号： A61K39/385 ,  A61K2039/6025 ,  Y02A50/386

摘要： The present invention is related to methods and compositions that are capable of immediately immunizing a human or animal against any molecule or compound. The present invention comprises an immunity linker molecule with at least two sites; (1) a first binding site that binds to an immune system molecule in a human or animal that has been preimmunized against the first binding site, and (2) one or more second binding sites that bind specifically to a desired compound or molecule. The first binding site and the second binding site(s) are linked by a linker portion of the molecule.

摘要翻译： 本发明涉及能够立即针对任何分子或化合物免疫人或动物的方法和组合物。 本发明包括具有至少两个位点的免疫接头分子; （1）结合已针对第一结合位点预免疫的人或动物中的免疫系统分子的第一结合位点，和（2）特异性结合所需化合物或分子的一个或多个第二结合位点。 第一结合位点和第二结合位点通过分子的接头部分连接。

公开(公告)号：US07422746B2

公开(公告)日：2008-09-09

申请号：US10696770

申请日：2003-10-29

申请人： Kary B. Mullis

发明人： Kary B. Mullis

IPC分类号： A61K39/00

CPC分类号： A61K39/385 ,  A61K2039/6025 ,  Y02A50/386

摘要： Methods and compositions for immediately immunizing an individual against any molecule or compound. The present invention comprises an immunity linker with at least two sites; (1) at least one first binding site that binds to an immune response component in an individual that has been pre-immunized with a universal immunogen, and (2) at least one second binding site that binds specifically to a desired compound or molecule, the target.

摘要翻译： 立即将个体免疫任何分子或化合物的方法和组合物。 本发明包括具有至少两个位点的免疫接头; （1）至少一个第一结合位点，其结合已经用通用免疫原预先免疫的个体中的免疫应答组分，和（2）至少一个特异性结合所需化合物或分子的第二结合位点， 目标。

公开(公告)号：US06197563B1

公开(公告)日：2001-03-06

申请号：US08345367

申请日：1994-11-18

申请人： Henry A. Erlich ,  Glenn Horn ,  Randall K. Saiki ,  Kary B. Mullis ,  David H. Gelfand

发明人： Henry A. Erlich ,  Glenn Horn ,  Randall K. Saiki ,  Kary B. Mullis ,  David H. Gelfand

IPC分类号： C12N912

CPC分类号： C12P19/34 ,  B01J19/0046 ,  B01J2219/00495 ,  B01J2219/0059 ,  B01J2219/00689 ,  B01J2219/00722 ,  B01L7/52 ,  C07H21/00 ,  C07K14/70539 ,  C07K14/805 ,  C12N9/1252 ,  C12N15/10 ,  C12Q1/6846 ,  C40B40/06 ,  C40B60/14

摘要： The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

摘要翻译： 本发明涉及使用热稳定性酶扩增核酸或其混合物中包含的任何靶核酸序列的方法。 该方法包括用摩尔过量的两种寡核苷酸引物处理核酸的分开的互补链，用热稳定酶扩增引物以形成互补引物延伸产物，其用作合成所需核酸序列的模板，并检测序列 放大 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。

公开(公告)号：US5176995A

公开(公告)日：1993-01-05

申请号：US394145

申请日：1989-08-15

申请人： John J. Sninsky ,  Shirley Y. Kwok ,  David H. Mack ,  Henry A. Ehrlich ,  Kary B. Mullis

发明人： John J. Sninsky ,  Shirley Y. Kwok ,  David H. Mack ,  Henry A. Ehrlich ,  Kary B. Mullis

IPC分类号： B01L7/00 ,  C07K14/805 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/70 ,  C40B40/06 ,  C40B60/14

CPC分类号： B01L7/52 ,  C07K14/805 ,  C12N15/10 ,  C12Q1/6823 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883 ,  C12Q1/70 ,  C12Q1/703 ,  C12Q1/706 ,  B01J2219/00495 ,  B01J2219/0059 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/81 ,  Y10S436/811

摘要： The presence or absence of a nucleic acid sequence associated with one or more related viruses in a sample containing one or more nucleic acids and suspected of containing such sequence can be detected by amplifying the sequence using primers to form extension products as templates and detecting the amplified product if it is present. This may be accomplished by adding a labeled hybridization probe to the amplified product either free in solution or after immobilization on a solid support. Preferably the virus constitutes AIDs viruses and hepadnaviruses.

摘要翻译： 可以通过使用引物扩增序列来检测含有一种或多种核酸并且怀疑含有该序列的样品中与一种或多种相关病毒相关的核酸序列，以形成扩增产物作为模板并检测扩增的 产品如果存在的话。 这可以通过将标记的杂交探针加入到在溶液中游离或固定在固体支持物上的扩增产物来实现。 优选地，病毒构成AIDs病毒和肝炎病毒。

公开(公告)号：US4683195A

公开(公告)日：1987-07-28

申请号：US828144

申请日：1986-02-07

申请人： Kary B. Mullis ,  Henry A. Erlich ,  Norman Arnheim ,  Glenn T. Horn ,  Randall K. Saiki ,  Stephen J. Scharf

发明人： Kary B. Mullis ,  Henry A. Erlich ,  Norman Arnheim ,  Glenn T. Horn ,  Randall K. Saiki ,  Stephen J. Scharf

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N1/00 ,  C12N15/00 ,  G01N33/48 ,  G01N33/00 ,  G01N33/566 ,  G01N33/564 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6881 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2600/156 ,  Y10T436/143333

摘要： The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.In addition, a specific nucleic acid sequence may be cloned into a vector by using primers to amplify the sequence, which contain restriction sites on their non-complementary ends, and a nucleic acid fragment may be prepared from an existing shorter fragment using the amplification process.

摘要翻译： 本发明涉及扩增和检测包含在核酸或其混合物中的任何靶核酸序列的方法。 该方法包括用摩尔过量的两种寡核苷酸引物处理分离的核酸互补链，延伸引物以形成用作合成所需核酸序列的模板的互补引物延伸产物，以及检测如此扩增的序列。 反应的步骤可以逐步或同时进行，并且可以根据需要经常重复。 此外，通过使用引物扩增其非互补末端含有限制位点的序列，可以将特异性核酸序列克隆到载体中，并且可以使用扩增过程从现有的较短片段制备核酸片段 。

公开(公告)号：US4582789A

公开(公告)日：1986-04-15

申请号：US683263

申请日：1984-12-18

申请人： Edward L. Sheldon, III ,  Corey H. Levenson ,  Kary B. Mullis ,  Henry Rapoport

发明人： Edward L. Sheldon, III ,  Corey H. Levenson ,  Kary B. Mullis ,  Henry Rapoport

IPC分类号： C07D493/04 ,  C07D513/04 ,  C07D519/00 ,  C07H21/00 ,  C12Q1/68 ,  G01N33/566

CPC分类号： C07D493/04 ,  C07D513/04 ,  C07H21/00

摘要： A labeling reagent of the formula:[A][B]Lis prepared where A is an alkylating intercalation moiety, B is a divalent organic spacer arm moiety with a straight chain of at least two carbon atoms, and L is a monovalent label moiety capable of producing a detectable signal, e.g., a signal detectable by spectroscopic, photochemical, chemical, immunochemical or biochemical means. Preferably A is a 4'-methylene-substituted psoralen moiety, and most preferably A is a 4'-methylene-substituted 4,5',8-trimethylpsoralen moiety.This reagent may be used to label nucleic acids, preferably DNA, by intercalating the alkylating intercalation moiety of the reagent into an at least partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hydridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive.This reagent may also be used in chromosome banding to label specific regions of chromosomes and thereby differentiate them.