公开(公告)号：US20070117115A1

公开(公告)日：2007-05-24

申请号：US11488633

申请日：2006-07-19

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is A. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second and the third bases from the 3′ end to be 5′-AT-3′, 5′-GT-3′, 5′-CA-3′ or 5′-CT-3′; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

公开(公告)号：US20070042393A1

公开(公告)日：2007-02-22

申请号：US11333352

申请日：2006-01-18

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6813

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is T. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of T, C or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

公开(公告)号：US20070037176A1

公开(公告)日：2007-02-15

申请号：US11203417

申请日：2005-08-15

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6858 ,  C12Q2535/131 ,  C12Q2535/125

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is G. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of A, T or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

公开(公告)号：US07497996B2

公开(公告)日：2009-03-03

申请号：US11516008

申请日：2006-09-06

申请人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Tetsuo Yukimasa ,  Hidenobu Yaku ,  Maki Yotsuhashi ,  Yukari Hataoka

发明人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Tetsuo Yukimasa ,  Hidenobu Yaku ,  Maki Yotsuhashi ,  Yukari Hataoka

IPC分类号： B01L3/02

CPC分类号： G01N35/00069 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0806 ,  B01L2400/0409 ,  B01L2400/0412 ,  B01L2400/0688 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0057 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T436/111666 ,  Y10T436/2575

摘要： A liquid delivery apparatus 1 has a rotary substrate 2. The substrate 2 formed with a fluid passage 8A for communicating a supply chamber 6A and target chamber 7A. An inlet end portion 13 at which the fluid passage 8A is connected to the chamber 6A extends in a clockwise direction R1 of rotational directions of the substrate 2. The inlet end portion 13 holds a liquid 9 in the chamber 6A by a capillary force. The rotation drive unit 4 rotates the substrate 2 so that an inertial force exceeding the capillary force and directed to the clockwise direction R1 acts on the liquid 9 in the inlet end portion 13. Delivery behavior control of microfluid with a high degree of flexibility can be achieved.

摘要翻译： 液体输送装置1具有旋转基板2.基板2形成有用于连通供给室6A和目标室7A的流体通道8A。 流体通道8A连接到腔室6A的入口端部13沿着衬底2的旋转方向的顺时针方向R1延伸。入口端部13通过毛细管力将液体9保持在腔室6A中。 旋转驱动单元4旋转基板2，使得超过毛细管力并且指向顺时针方向R1的惯性力作用在入口端部13中的液体9上。具有高柔性的微流体的输送行为控制可以是 实现了

公开(公告)号：US07294470B2

公开(公告)日：2007-11-13

申请号：US11489583

申请日：2006-07-20

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is G.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second and the third bases from the 3′ end to be 5′-AT-3′, 5′-TT-3′, 5′-GA-3′ or 5′-GT-3′; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 一个基座间隔开的是G.根据本发明，3'端基被设计为对应于SNP的基极; 3'末端的第2个碱基和第3个碱基为5'-AT-3'，5'-TT-3'，5'-GA-3'或5'-GT-3' 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

公开(公告)号：US07294469B2

公开(公告)日：2007-11-13

申请号：US11488633

申请日：2006-07-19

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is A.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second and the third bases from the 3′ end to be 5′-AT-3′, 5′-GT-3′, 5′-CA-3′ or 5′-CT-3′; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 一个分隔开的基底是A.根据本发明，3'端基被设计为对应于SNP的碱基; 3'末端的第2个和第3个碱基为5'-AT-3'，5'-GT-3'，5'-CA-3'或5'-CT-3' 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

公开(公告)号：US20070117117A1

公开(公告)日：2007-05-24

申请号：US11489582

申请日：2006-07-20

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is T. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T; the third base from the 3′ end to be any one of T, G or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开。根据本发明，3'端基被设计为对应于SNP的基座; 从3'端的第二个基地是T; 3'末端的第3个碱基为T，G或C任一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

公开(公告)号：US20060263247A1

公开(公告)日：2006-11-23

申请号：US11494749

申请日：2006-07-28

申请人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Tetsuo Yukimasa ,  Hidenobu Yaku ,  Maki Yotsuhashi ,  Yukari Hataoka

发明人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Tetsuo Yukimasa ,  Hidenobu Yaku ,  Maki Yotsuhashi ,  Yukari Hataoka

IPC分类号： G01N33/00

CPC分类号： G01N35/00069 ,  Y10T436/111666 ,  Y10T436/2575

摘要： The present invention provides a blood processing apparatus that can introduce a metered quantity of a blood sample into a chamber and does not require an opening linked to the chamber to be closed after the introduction of the blood sample. An introducing chamber, an injection port, and an air port are formed in a rotatable platform of a blood processing apparatus. The injection port links the introducing chamber with the outside of the rotatable platform. A blood sample is introduced from the injection port into the introducing chamber. A blood coagulant is held on the port wall surface of the air port and/or around a portion of the surface of the rotatable platform where the air port is open.

摘要翻译： 本发明提供一种血液处理装置，其能够将计量量的血液样品引入腔室中，并且在引入血液样本之后不需要连接到待关闭的腔室的开口。 在血液处理装置的可旋转平台中形成有引入室，注射口和空气口。 注入口将引入室与可旋转平台的外部连接。 血液样品从注射口引入引入室。 血液凝结剂被保持在空气端口的端口壁表面上和/或在空气端口打开的可旋转平台的表面的一部分周围。

公开(公告)号：US20050032075A1

公开(公告)日：2005-02-10

申请号：US10674787

申请日：2003-10-01

申请人： Hidenobu Yaku ,  Tetsuo Yukimasa ,  Hiroaki Oka

发明人： Hidenobu Yaku ,  Tetsuo Yukimasa ,  Hiroaki Oka

IPC分类号： C12Q1/25 ,  C12Q1/68 ,  G01N33/00

CPC分类号： C12Q1/6858 ,  C12Q1/6869 ,  C12Q2565/301 ,  C12Q2521/543

摘要： Convenient techniques for discriminating the base type in a base sequence of a nucleic acid are provided. The technique includes the step (a) of preparing a sample solution containing a nucleic acid, a primer having a base sequence that includes a complementary binding region which complementarily binds to the nucleic acid, and a nucleotide; the step (b) of allowing the sample solution to stand under a condition to cause an extension reaction of the primer, and producing pyrophosphate when the extension reaction is caused; the step (c) of bringing the sample solution into contact with the front face of a H+ hardly permeable membrane having H+-pyrophosphatase, which penetrates from front to back of the membrane, of which active site that hydrolyzes pyrophosphate being exposed to the front face; the step (d) of measuring the H+ concentration of at least either one of the solution at the front face side of the H+ hardly permeable membrane or the solution at the back face side of the H+ hardly permeable membrane, in a state where the H+-pyrophosphatase is immersed in the solution; the step (e) of detecting the extension reaction on the basis of the result of measurement in the step (d) ; and the step (f) of discriminating the base type in the base sequence of the nucleic acid on the basis of the result of detection in the step (e).

摘要翻译： 提供了用于区分核酸的碱基序列中的碱基类型的方便的技术。 该技术包括制备含有核酸的样品溶液的步骤（a），具有与核酸互补结合的互补结合区的碱基序列的引物和核苷酸; 在引起引物延伸反应的条件下使样品溶液静置的步骤（b），在引起延伸反应时产生焦磷酸盐; 使样品溶液与H + - 焦磷酸酶的前端面从H + - 焦磷酸酶的前面向后表面接触的步骤（c），其中水解焦磷酸盐的活性位点 暴露在前面; 在H +难以渗透的膜的前面侧的溶液或H +的背面侧的溶液中的至少任一溶液的H +浓度的测定步骤（d） 在其中将H + - 焦磷酸酶浸入溶液中的状态下， 基于步骤（d）中的测量结果检测延伸反应的步骤（e）; 以及基于步骤（e）中的检测结果来区分核酸碱基序列中的碱基类型的步骤（f）。

公开(公告)号：US20080293128A1

公开(公告)日：2008-11-27

申请号：US12112249

申请日：2008-04-30

申请人： Tetsuo YUKIMASA ,  Hiroaki Oka

发明人： Tetsuo YUKIMASA ,  Hiroaki Oka

IPC分类号： C12M1/00

CPC分类号： C12Q1/6827 ,  C12Q2565/301

摘要： A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided.A sensor of pyrophosphate which is characterized by including: an insulative substrate 1; an electrode system that is formed thereon and has a measurement electrode 2 and a counter electrode 3; and a plurality of reaction reagent layers that are provided on the substrate 1 and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer 36 including the enzyme being separated from reaction reagent layer 35 including the buffer component, and reaction reagent layer 37 including glyceraldehyde-3-phosphate being separated from the reaction reagent layer 35 including the buffer component.

摘要翻译： 提供了一种焦磷酸盐传感器，其可以在使用引物延伸反应的SNP分型中测量焦磷酸盐的方法中以高灵敏度方便地检测焦磷酸盐。 焦磷酸盐传感器，其特征在于包括：绝缘基板1; 形成在其上并具有测量电极2和对电极3的电极系统; 以及设置在基板1上的多个反应试剂层，包括焦磷酸酶，甘油醛-3-磷酸脱氢酶，心肌黄酶，甘油醛-3-磷酸，氧化尼古丁胺腺嘌呤二核苷酸，电子介体，镁盐和缓冲成分， 将包含缓冲成分的反应试剂层35分离出的酶的反应试剂层36和包含甘油醛-3-磷酸的反应试剂层37与包含缓冲成分的反应试剂层35分离。