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    Compositions for isolating a cDNA encoding a membrane-bound protein
    24.
    发明授权
    Compositions for isolating a cDNA encoding a membrane-bound protein 失效
    用于分离编码膜结合蛋白的cDNA的组合物

    公开(公告)号：US07338797B2

    公开(公告)日：2008-03-04

    申请号：US10425529

    申请日：2003-04-29

    申请人： Masayuki Tsuchiya ,  Mikiyoshi Saito ,  Toshihiko Ohtomo

    发明人： Masayuki Tsuchiya ,  Mikiyoshi Saito ,  Toshihiko Ohtomo

    IPC分类号： C07H21/04

    CPC分类号： C12N15/1051 ,  C07K14/705 ,  C07K16/00 ,  C07K16/2866 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/1034

    摘要： A method, vector, and kit are provided for selectively isolating genes encoding membrane-bound proteins by fusing proteins to a secretable protein having a binding affinity to an antigen (e.g., an antibody), expressing these fusions in host cells, and selectively isolating individual host cells by their ability to bind the antigen via the fusion protein. Host cells expressing fusion proteins that do not contain a membrane binding domain will be relatively unable to bind the antigen, since the fusion proteins in those cells are secreted and unattached to their host cells. The method, vector, and kit disclosed herein are particularly useful for identifying genes for membrane-bound proteins represented in cDNA libraries.

    摘要翻译： 提供了一种方法，载体和试剂盒，用于通过将蛋白质融合到对抗原（例如抗体）具有结合亲和力的可分泌蛋白质来选择性分离编码膜结合蛋白的基因，在宿主细胞中表达这些融合物，并选择性分离个体 宿主细胞通过其融合蛋白结合抗原的能力。 表达不含膜结合结构域的融合蛋白的宿主细胞将相对不能结合抗原，因为那些细胞中的融合蛋白被分泌并且不附着于其宿主细胞。 本文公开的方法，载体和试剂盒特别可用于鉴定cDNA文库中表达的膜结合蛋白的基因。

    Methods for isolating a cDNA encoding a membrane-bound protein
    26.
    发明授权
    Methods for isolating a cDNA encoding a membrane-bound protein 失效
    分离编码膜结合蛋白的cDNA的方法

    公开(公告)号：US06610485B1

    公开(公告)日：2003-08-26

    申请号：US09700820

    申请日：2001-05-02

    申请人： Masayuki Tsuchiya ,  Mikiyoshi Saito ,  Toshihiko Ohtomo

    发明人： Masayuki Tsuchiya ,  Mikiyoshi Saito ,  Toshihiko Ohtomo

    IPC分类号： C12Q168

    CPC分类号： C12N15/1051 ,  C07K14/705 ,  C07K16/00 ,  C07K16/2866 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/1034

    摘要： A method and kit are provided for selectively isolating genes encoding membrane-bound proteins by fusing proteins to a secretable protein having a binding affinity to an antigen (e.g., an antibody), expressing these fusions in host cells, and selectively isolating individual host cells by their ability to bind the antigen via the fusion protein. Host cells expressing fusion proteins that do not contain a membrane binding domain will be relatively unable to bind the antigen, since the fusion proteins in those cells are secreted and unattached to their host cells. The method and kit disclosed herein are particularly useful for identifying genes for membrane-bound proteins represented in cDNA libraries.

    摘要翻译： 提供了一种方法和试剂盒，用于通过将蛋白质融合到对抗原（例如抗体）具有结合亲和力的可分泌蛋白质来选择性分离编码膜结合蛋白的基因，在宿主细胞中表达这些融合物，并通过 它们通过融合蛋白结合抗原的能力。 表达不含膜结合结构域的融合蛋白的宿主细胞将相对不能结合抗原，因为那些细胞中的融合蛋白被分泌并且不附着于其宿主细胞。 本文公开的方法和试剂盒特别可用于鉴定cDNA文库中表达的膜结合蛋白的基因。

    Reshaped human antibody to human medulloblastoma cells
    28.
    发明授权
    Reshaped human antibody to human medulloblastoma cells 失效
    人类成神经管细胞瘤细胞重构人抗体

    公开(公告)号：US06214973B1

    公开(公告)日：2001-04-10

    申请号：US08646265

    申请日：1996-09-09

    申请人： Toshihiko Ohtomo ,  Koh Sato ,  Masayuki Tsuchiya

    发明人： Toshihiko Ohtomo ,  Koh Sato ,  Masayuki Tsuchiya

    IPC分类号： C07K1600

    CPC分类号： C07K16/3053 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2319/00

    摘要： The present invention discloses reshaped antibody to human medulloblastoma cells comprising: (A) an L chain comprising: (1) a human L chain C region, and (2) an L chain V region comprising human L chain FRs and L chain CDRs of mouse monoclonal antibody ONS-M21 to human medulloblastoma cells; and, (B) an H chain containing: (1) a human H chain C region, and (2) an H chain V region comprising human H chain FRs and H chain CDRs of mouse monoclonal antibody ONS-M21 to human medulloblastoma cells. Since the majority of this reshaped human antibody is derived from a human antibody and mouse CDRs have a low level of antigenicity, the reshaped human antibody of the present invention has a low level of antigenicity in humans, and is therefore expected to be useful as a therapeutic agent and diagnostic tool for brain tumors such as medulloblastoma which strongly express antigen that is recognized by this antibody.

    摘要翻译： 本发明公开了对人成神经管细胞瘤细胞重整的抗体，其包含：（A）L链，其包含：（1）人L链C区，和（2）包含人L链FR和L链CDR的L链V区 单克隆抗体ONS-M21给人成神经管细胞瘤细胞; 和（B）包含以下物质的H链：（1）人H链C区，和（2）包含人H链FR的H链V区和小鼠单克隆抗体ONS-M21的H链CDR至人成神经管细胞瘤细胞。 由于这种重整的人抗体的大部分来自人抗体，小鼠CDR具有低水平的抗原性，所以本发明的重构人抗体在人体中具有低水平的抗原性，因此预期可用作 用于脑肿瘤如成神经管细胞瘤的治疗剂和诊断工具，其能强烈表达由该抗体识别的抗原。