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21.发明授权公开(公告)号：US08148121B2
公开(公告)日：2012-04-03
申请号：US12512429
申请日：2009-07-30
CPC分类号： C12N9/0083 , C12N9/0071 , C12P7/6427 , C12Y114/19003
摘要： The present invention relates to Δ6 desaturases, which have the ability to convert linoleic acid [“LA”; 18:2 ω-6] to γ-linolenic acid [“GLA”; 18:3 ω-6] and/or α-linolenic acid [“ALA”; 18:3 ω-3] to stearidonic acid [“STA”; 18:4 ω-3]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ6 desaturases, along with methods of making long-chain polyunsaturated fatty acids [“PUFAs”] using these Δ6 desaturases in oleaginous yeast, are disclosed.
摘要翻译： 本发明涉及具有转化亚油酸[“LA”; 18：2ω-6]与γ-亚麻酸[“GLA” 18：3ω-6]和/或α-亚麻酸[“ALA”; 18：3ω-3]至十八碳四烯酸[“STA”; 18：4ω-3]。 公开了分离的核酸片段和包含编码“Dgr”6去饱和酶的片段的重组构建体以及使用这些＆Dgr。6去饱和酶在含油酵母中制备长链多不饱和脂肪酸的方法。
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22.发明授权Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 失效用于改变多不饱和脂肪酸的二酰基甘油酰基转移酶和含油生物体中的油含量公开(公告)号：US07273746B2
公开(公告)日：2007-09-25
申请号：US11024544
申请日：2004-12-29
申请人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
发明人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
CPC分类号： C12N9/1029 , C12P7/6463 , C12P7/6472
摘要： Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
摘要翻译： 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。
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23.发明授权Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权用于改变多不饱和脂肪酸的二酰基甘油酰基转移酶和含油生物体中的油含量公开(公告)号：US08685681B2
公开(公告)日：2014-04-01
申请号：US12338536
申请日：2008-12-18
申请人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
发明人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
CPC分类号： C12N9/1029 , C12P7/6463 , C12P7/6472
摘要： Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
摘要翻译： 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。
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公开(公告)号：US20090311380A1
公开(公告)日：2009-12-17
申请号：US12466072
申请日：2009-05-14
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。
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25.发明申请Diacylglycerol Acyltransferases for Alteration of Polyunsaturated Fatty Acids and Oil Content in Oleaginous Organisms 有权用于改变多不饱和脂肪酸的二酰基甘油酰基转移酶和油性生物中的油含量公开(公告)号：US20090104674A1
公开(公告)日：2009-04-23
申请号：US12338536
申请日：2008-12-18
申请人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
发明人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
IPC分类号： C12P7/64
CPC分类号： C12N9/1029 , C12P7/6463 , C12P7/6472
摘要： Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
摘要翻译： 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。
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26.发明申请公开(公告)号：US20060263867A1
公开(公告)日：2006-11-23
申请号：US11494318
申请日：2006-07-27
申请人： Narendra Yadav , Hongxiang Zhang
发明人： Narendra Yadav , Hongxiang Zhang
CPC分类号： C12N9/0083
摘要： The present invention relates to a Δ12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific ω-3 and/or ω-6 fatty acids are described by overexpression of the Δ12 fatty acid desaturase or by disruption of the native gene.
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27.发明申请公开(公告)号：US20060160193A1
公开(公告)日：2006-07-20
申请号：US10882760
申请日：2004-07-01
申请人： Narendra Yadav , Hongxiang Zhang
发明人： Narendra Yadav , Hongxiang Zhang
CPC分类号： C12P7/6481 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Two acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, the genes encoding phophatidylcholine-diacylglycerol acyltransferase (PDAT) and diacylglycerol acyltransferase (DGAT2) have been isolated from Y. lipolytica. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
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28.发明申请Delta 15 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous plants and yeast 有权适用于改变含油植物和酵母中多不饱和脂肪酸水平的Delta 15去饱和酶公开(公告)号：US20050132442A1
公开(公告)日：2005-06-16
申请号：US10985254
申请日：2004-11-10
申请人： Narendra Yadav , Hongxiang Zhang
发明人： Narendra Yadav , Hongxiang Zhang
IPC分类号： A01H1/00 , A01H5/00 , C07H21/04 , C12N20060101 , C12N1/18 , C12N5/04 , C12N9/02 , C12N9/04 , C12N15/74 , C12N15/82 , C12P7/64
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.
摘要翻译： 本发明涉及能够催化亚油酸（18:2，LA）转化为α-亚麻酸（18:3，ALA）的真菌Δ-15脂肪酸去饱和酶。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主植物和微生物。 本文还描述了通过过表达Δ-15脂肪酸去饱和酶增加特定ω-3和ω-6脂肪酸的产生的方法。
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29.发明申请Delta-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts 有权适用于改变含油酵母中多不饱和脂肪酸水平的Delta-12去饱和酶基因公开(公告)号：US20050043527A1
公开(公告)日：2005-02-24
申请号：US10840325
申请日：2004-05-06
申请人： Narendra Yadav , Hongxiang Zhang
发明人： Narendra Yadav , Hongxiang Zhang
IPC分类号： C07H21/04 , C12N20060101 , C12N9/02 , C12P20060101 , C12P7/64
CPC分类号： C12N9/0083
摘要： The present invention relates to a Δ12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific ω-3 and/or ω-6 fatty acids are described by overexpression of the Δ12 fatty acid desaturase or by disruption of the native gene.
摘要翻译： 本发明涉及能够催化油酸向亚油酸转化的Δ12脂肪酸去饱和酶（LA; 18：2）。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主微生物。 通过Δ12脂肪酸去饱和酶的过表达或天然基因的破坏来描述增加特定ω-3和/或ω-6脂肪酸生产的方法。
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30.发明申请Delta-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts 审中-公开适用于改变含油酵母中多不饱和脂肪酸水平的Delta-12去饱和酶基因公开(公告)号：US20060263866A1
公开(公告)日：2006-11-23
申请号：US11494317
申请日：2006-07-27
申请人： Narendra Yadav , Hongxiang Zhang
发明人： Narendra Yadav , Hongxiang Zhang
CPC分类号： C12N9/0083
摘要： The present invention relates to a Δ12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific ω-3 and/or ω-6 fatty acids are described by overexpression of the Δ12 fatty acid desaturase or by disruption of the native gene.
摘要翻译： 本发明涉及能够催化油酸向亚油酸转化的Δ12脂肪酸去饱和酶（LA; 18：2）。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主微生物。 通过Δ12脂肪酸去饱和酶的过表达或天然基因的破坏来描述增加特定ω-3和/或ω-6脂肪酸生产的方法。
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