公开(公告)号：US20210146354A1

公开(公告)日：2021-05-20

申请号：US17254462

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Merek Siu ,  Ali Agah ,  Stanley Hong ,  Tarun Khurana ,  Aathavan Karunakaran ,  Craig Ciesla

IPC: B01L3/00

Abstract: Devices, systems, and methods for non-volatile storage include a well activation device operable to modify one or more wells from a plurality of wells of a flow cell to provide a set of readable wells. Readable wells are configured to allow exposure of a well to substances from nucleotide sequencing fluids, and prevent exposure to other substances and fluids, such as nucleotide synthesizing fluids. The well activation device may also modify wells to provide a set of writeable wells. This set of wells is configured to allow exposure to the nucleotide synthesizing fluids and substances; and prevent exposure to the nucleotide sequencing fluids and substances. There may also be provisions made for risk mitigation for data errors such as generating commands to write specified data to a nucleotide sequence associated with a particular location in a storage device, reading the nucleotide sequence and performing a comparison.

公开(公告)号：US20250108375A1

公开(公告)日：2025-04-03

申请号：US18979010

申请日：2024-12-12

Applicant: ILLUMINA, INC.

Inventor： Aathavan Karunakaran ,  Arnaud Rival ,  Ali Agah ,  Fabien Abeille ,  Steven Barnard ,  Craig Ciesla ,  Murali Venkatesan ,  Pargol Gheissari ,  Jennifer Wang ,  Dietrich Dehlinger

IPC: B01L3/00

Abstract: A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.

公开(公告)号：US20230285974A1

公开(公告)日：2023-09-14

申请号：US18103625

申请日：2023-01-31

Applicant: ILLUMINA, INC.

Inventor： Merek Siu ,  Ali Agah ,  Stanley Hong ,  Tarun Khurana ,  Aathavan Karunakaran ,  Craig Ciesla ,  Amirali Kia

IPC: B01L3/00 ,  G16B50/30 ,  G11C13/02

CPC classification number: B01L3/50855 ,  G11C13/02 ,  G16B50/30 ,  B01L2300/0645 ,  B01L2300/0829 ,  C12Q1/6869

Abstract: Devices, systems, and methods for non-volatile storage include a well activation device operable to modify one or more wells from a plurality of wells of a flow cell to provide a set of readable wells. Readable wells are configured to allow exposure of a well to substances from nucleotide sequencing fluids, and prevent exposure to other substances and fluids, such as nucleotide synthesizing fluids. The well activation device may also modify wells to provide a set of writeable wells. This set of wells is configured to allow exposure to the nucleotide synthesizing fluids and substances; and prevent exposure to the nucleotide sequencing fluids and substances. There may also be provisions made for risk mitigation for data errors such as generating commands to write specified data to a nucleotide sequence associated with a particular location in a storage device, reading the nucleotide sequence and performing a comparison.

公开(公告)号：US20230226553A1

公开(公告)日：2023-07-20

申请号：US18002427

申请日：2021-08-31

Applicant: ILLUMINA, INC.

Inventor： Steven Barnard ,  Craig Ciesla ,  Paul Crivelli ,  Justin Davidson ,  Darren Segale

IPC: B01L9/06

CPC classification number: B01L9/06 ,  B01L2200/025 ,  B01L2200/04 ,  B01L2200/143 ,  B01L2300/021

Abstract: Cartridges and related systems and methods are disclosed. In accordance with an implementation, a method includes securing a sample container in a sample container receptacle of a cartridge using a sample container lock and coupling the cartridge to a cartridge receptacle of a system. The method also includes depositing a sample from the sample container within a sample well of the cartridge and determining a presence of a target molecule within the sample using the system. In response to the target molecule being present within the sample, the method includes releasing the sample container lock of the cartridge to allow the sample container to be removed from the sample container receptacle and for the cartridge to remain coupled to the system.

公开(公告)号：US20220003728A1

公开(公告)日：2022-01-06

申请号：US17479342

申请日：2021-09-20

Applicant: Illumina, Inc.

Inventor： Tarun Khurana ,  Ali Agah ,  Aathavan Karunakaran ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: G01N30/74 ,  C12Q1/6874

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

公开(公告)号：US20210362151A1

公开(公告)日：2021-11-25

申请号：US17255819

申请日：2020-02-07

Applicant: ILLUMINA, INC.

Inventor： Aathavan Karunakaran ,  Arnaud Rival ,  Ali Agah ,  Fabien Abeille ,  Steven Barnard ,  Craig Ciesla ,  Murali Venkatesan ,  Pargol Gheissari ,  Jennifer Wang ,  Dietrich Dehlinger

IPC: B01L3/00

Abstract: A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.

公开(公告)号：US11143638B2

公开(公告)日：2021-10-12

申请号：US17254466

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Tarun Khurana ,  Ali Agah ,  Aathavan Karunakaran ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: G01N30/74 ,  C12Q1/6874

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

公开(公告)号：US20210151129A1

公开(公告)日：2021-05-20

申请号：US17254459

申请日：2020-05-26

Applicant: ILLUMINA, INC.

Inventor： Craig Ciesla ,  Ali Agah ,  Stanley Hong ,  Tarun Khurana ,  Aathavan Karunakaran ,  Arvin Emadi ,  Merek Siu

IPC: G16B50/30 ,  G06F3/06 ,  G16B30/10 ,  C12Q1/6869

Abstract: A system writes input data to a storage device as machine-written polynucleotides; and reads machine written polynucleotides from the storage device as output data. The storage device includes a flow cell including a plurality of storage wells in which machine written polynucleotides may be stored. The storage device may include a set of electrodes corresponding to the storage wells that allow for selective interactions with wells across the surface of a flow cell. Operation of the storage device may include receiving a read request associated with a particular location in the storage device, creating a copy of a nucleotide sequence located at the particular location in the storage device, transferring the copy of the nucleotide sequence to a read location, and reading the copy of the nucleotide sequence at the read location.