公开(公告)号：US11351551B2

公开(公告)日：2022-06-07

申请号：US16626138

申请日：2018-10-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Andrew Joad ,  Daniel L. Fuller ,  Paul Crivelli

IPC: B01L3/00

Abstract: Disclosed are multi-well plate inserts that can be used to separate solid debris, including paper punch containing a blood sample, from a liquid containing target biological molecules, such as nucleic acid molecules and proteins. Also provided are methods of using the insert, for example as part of a method that analyzes target biological molecules.

公开(公告)号：US11291998B2

公开(公告)日：2022-04-05

申请号：US17165757

申请日：2021-02-02

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Wesley A. Cox-Muranami ,  Darren Robert Segale ,  Oliver Jon Miller ,  Jennifer Olivia Foley ,  Tarun Kumar Khurana ,  Paul Crivelli ,  Gary Watts

IPC: B01L3/00 ,  G01N1/31 ,  B01L9/00

Abstract: An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

公开(公告)号：USD843009S1

公开(公告)日：2019-03-12

申请号：US29581055

申请日：2016-10-14

Applicant: Illumina, Inc.

Designer： Gary Watts ,  Paul Crivelli ,  Anna Quackenbush

公开(公告)号：US12296333B2

公开(公告)日：2025-05-13

申请号：US18003284

申请日：2022-02-18

Applicant: Illumina, Inc.

Inventor： Jonathan Ziebarth ,  Jon Aday ,  Paul Crivelli ,  Gerald Kreindl ,  Amit Sharma

IPC: B29C45/14 ,  B01L3/00 ,  B29C45/00

Abstract: Provided herein include various examples of a method for manufacturing aspects of flow cell. The method may include performing chemical processes on a surface of the patterned wafer to prepare the surface of the patterned, singulating the wafer into individual dies, orienting each die on a temporary substrate, where the orienting creates spaces between each individual die, and molding a material over the spaces to create a hybrid wafer comprised of glass and molded material. The method may also include bonding two of the hybrid wafers together, forming a bonded wafer stack.

公开(公告)号：US20240299936A1

公开(公告)日：2024-09-12

申请号：US18280651

申请日：2022-03-01

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Paul Crivelli ,  Justin Davidson ,  Sébastien Ricoult

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2200/16 ,  B01L2300/123 ,  B01L2400/0622 ,  B01L2400/088

Abstract: Well assemblies enabling optical access therein and related systems and methods are disclosed. In accordance with an implementation, an apparatus includes a body, dry reagent, and a cover. The body defines a well and has an opening, an aperture, and a field of view (FOV) enabling optical access from the aperture to the well. The dry reagent is contained within the well. The cover is coupled to the body and covering the opening.

公开(公告)号：US11951477B2

公开(公告)日：2024-04-09

申请号：US17809604

申请日：2022-06-29

Applicant: Illumina, Inc.

Inventor： Sz-Chin Lin ,  Jay Taylor ,  Minsoung Rhee ,  Jennifer Foley ,  Wesley Cox-Muranami ,  Cyril Delattre ,  Tarun Khurana ,  Paul Crivelli

IPC: B01L3/00 ,  G01N21/05

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502738 ,  G01N21/05 ,  B01L2300/0861 ,  B01L2400/0622 ,  B01L2400/08

Abstract: An example method includes connecting a flow cell to an instrument. The flow cell includes a flow channel including a manifold section having a manifold section swept volume and a detection section having a detection section swept volume. A ratio of the detection section swept volume to manifold section swept volume is at least 10 to 1. A first reagent is pumped through the flow channel. A first chemical reaction is performed between the first reagent and analytes positioned in the detection section. A subsequent reagent is pumped through the flow channel to flush out the remaining reagent. A concentration of at least 99.95 percent of reagent positioned in the detection section is the subsequent reagent, after pumping a total volume of the subsequent reagent through the flow channel that is equal to or less than 2.5 times a total swept volume of the manifold section plus the detection section.

公开(公告)号：US20230226553A1

公开(公告)日：2023-07-20

申请号：US18002427

申请日：2021-08-31

Applicant: ILLUMINA, INC.

Inventor： Steven Barnard ,  Craig Ciesla ,  Paul Crivelli ,  Justin Davidson ,  Darren Segale

IPC: B01L9/06

CPC classification number: B01L9/06 ,  B01L2200/025 ,  B01L2200/04 ,  B01L2200/143 ,  B01L2300/021

Abstract: Cartridges and related systems and methods are disclosed. In accordance with an implementation, a method includes securing a sample container in a sample container receptacle of a cartridge using a sample container lock and coupling the cartridge to a cartridge receptacle of a system. The method also includes depositing a sample from the sample container within a sample well of the cartridge and determining a presence of a target molecule within the sample using the system. In response to the target molecule being present within the sample, the method includes releasing the sample container lock of the cartridge to allow the sample container to be removed from the sample container receptacle and for the cartridge to remain coupled to the system.

公开(公告)号：US20220291250A1

公开(公告)日：2022-09-15

申请号：US17831222

申请日：2022-06-02

Applicant: Illumina, Inc.

Inventor： Wesley A. Cox-Muranami ,  James Osmus ,  Paul Crivelli ,  Bradley Kent Drews

IPC: G01N35/10

Abstract: An apparatus includes a fluidic circuit, a bypass fluidic circuit, a first set of fluid wells, a second set of fluid wells, a first valve, and a second valve. The first valve operatively associated with the first set of fluid wells such that the first selectively fluidly connects any one of the first set of fluid wells to a first valve outlet. The second valve operatively associated with the fluidic circuit, the bypass fluidic circuit, the first valve outlet, and the second set of fluid wells such that the second valve selectively fluidly connects any one of the second set of fluid wells and the first valve outlet to the fluidic circuit or the first valve outlet to the bypass fluidic circuit.

公开(公告)号：US20220168737A1

公开(公告)日：2022-06-02

申请号：US17539534

申请日：2021-12-01

Applicant: ILLUMINA, INC.

Inventor： Cameron Mar ,  Payam Taheri ,  Cyril Delattre ,  Paul Crivelli ,  Wesley Cox-Muranami ,  Zhenning Cao ,  Travis Beckman ,  Justin Davidson

IPC: B01L3/00

Abstract: Well assemblies and related methods are disclosed. In accordance with an implementation, an apparatus includes a liquid reservoir containing a liquid and a well assembly including a body, a hydrophobic venting membrane, and a cover. The body defines a well and has an opening and a port. The port is couplable to the liquid reservoir. The hydrophobic venting membrane is coupled to the body and covers the opening and cover covers the hydrophobic venting membrane. As the liquid is flowed into the well via the port, the hydrophobic venting membrane vents gas contained within the well.

公开(公告)号：US12070750B2

公开(公告)日：2024-08-27

申请号：US17981035

申请日：2022-11-04

Applicant: ILLUMINA, INC.

Inventor： Paul Crivelli ,  Cyril Delattre

IPC: B01L3/00 ,  G01N35/10

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502738 ,  G01N35/1002 ,  B01L2200/12 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/06

Abstract: An apparatus includes a fluid reservoir and a laminate fluidic circuit positioned above the fluid reservoir. The laminate fluidic circuit includes two or more layers laminated together to define a substantially planar substrate and one or more channels defined within the substrate. The laminate fluidic circuit includes a flexible conduit defined by a portion of the substrate encompassing an extent of at least one of the channels that is partially separated or separable from the remainder of the substrate. The flexible conduit is deflectable with respect to the planar substrate toward the fluid reservoir such that the flexible conduit fluidly connects the at least one channel to the fluid reservoir.