公开(公告)号：US09580747B2

公开(公告)日：2017-02-28

申请号：US14196332

申请日：2014-03-04

Applicant: Panasonic Corporation ,  IMEC vzw

Inventor： Hiroyuki Tanaka ,  Benjamin Jones ,  Paolo Fiorini

IPC: C12Q1/68 ,  B01L7/00 ,  B01L3/00

CPC classification number: C12Q1/686 ,  B01L3/502707 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/1805 ,  B01L2300/1883 ,  B01L2400/0439 ,  C12Q2565/629

Abstract: A DNA chip with micro-channel for DNA analysis of DNA included in an analyte according to a PCR method is a DNA chip with micro-channel for DNA analysis in which is silicon (first layer) and plastic (second layer) are laminated, wherein the second layer is formed on a partial area of the first layer, and the second layer includes: a reagent; a liquid transporting system; and a sensor, and the first layer includes a PCR reactor provided on an area on which the second layer is not formed.

Abstract translation: 具有根据PCR方法包含在分析物中的DNA进行DNA分析的微通道的DNA芯片是具有用于DNA分析的微通道的DNA芯片，其中层叠有硅（第一层）和塑料（第二层），其中 第二层形成在第一层的局部区域上，第二层包括：试剂; 液体输送系统; 和传感器，第一层包括设置在不形成第二层的区域上的PCR反应器。

公开(公告)号：US20150196909A1

公开(公告)日：2015-07-16

申请号：US14597716

申请日：2015-01-15

Applicant: IMEC VZW

Inventor： Benjamin Jones ,  Paolo Fiorini

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2300/0838 ,  B01L2300/12 ,  B01L2400/0406 ,  B01L2400/0688 ,  Y10T137/87917

Abstract: The embodiments of the present disclosure relate to a micro-fluidic device comprising a substrate, a cavity in the substrate and a plurality of micro-pillar columns located inside the cavity. The micro-pillars columns are configured to create a capillary action when a fluid sample is provided in the cavity. A micro-fluidic channel is present between two 5 walls of any two adjacent micro-pillars in a same micro-pillar column. Each of the two walls comprises a sharp corner along the direction of a propagation path of the fluid sample in the micro-fluidic channel thereby forming a capillary stop valve. A notch provided in a sidewall of the cavity acts as a capillary stop valve.

Abstract translation: 本公开的实施例涉及一种微流体装置，其包括衬底，衬底中的空腔和位于空腔内的多个微柱。 微柱柱配置成当在空腔中提供流体样品时产生毛细管作用。 在相同的微柱柱中的任何两个相邻微柱的两个5壁之间存在微流体通道。 两个壁中的每一个包括沿着微流体通道中的流体样品的传播路径的方向的尖角，从而形成毛细管截止阀。 设置在腔的侧壁中的凹口用作毛细管截止阀。

公开(公告)号：US20150151301A1

公开(公告)日：2015-06-04

申请号：US14547737

申请日：2014-11-19

Applicant: IMEC VZW

Inventor： Paolo Fiorini ,  Tim Stakenborg ,  Frederik Colle

IPC: B01L3/00 ,  C12Q1/68 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L2200/061 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/1827 ,  C12Q1/686

Abstract: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.

Abstract translation: 提出了一种用于执行数字PCR的微流体装置100。 该器件包括：半导体衬底; 第一微流体通道104，包括嵌入在半导体衬底中的入口102和出口103; 热耦合到第一微流体通道104的加热元件101; 液滴发生器107，其连接到第一微流体通道104的入口102，用于产生液滴并以流速泵送产生的液滴到第一微流体通道104中; 其特征在于：加热元件101是连接到温度控制单元111的单个加热元件，温度控制单元111被配置为使完整的第一微流体通道104的温度循环至少两个温度值; 并且其中液滴发生器107的流量适应。 此外，使用微流体装置100呈现执行数字PCR的方法。