公开(公告)号：US11142740B2

公开(公告)日：2021-10-12

申请号：US16536049

申请日：2019-08-08

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M3/00 ,  C12N15/00 ,  B01L3/00 ,  C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US20210310012A1

公开(公告)日：2021-10-07

申请号：US17353282

申请日：2021-06-21

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Charles Johnson ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US20210254105A1

公开(公告)日：2021-08-19

申请号：US17173137

申请日：2021-02-10

Applicant: Inscripta, Inc.

Inventor： Matthew Estes ,  Chad Sanada ,  Miles Gander ,  Eileen Spindler

IPC: C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited yeast cells in a cell population when employing nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US11066675B2

公开(公告)日：2021-07-20

申请号：US17140056

申请日：2021-01-02

Applicant: Inscripta, Inc.

Inventor： Krishna Kannan ,  Miles Gander ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/80 ,  C12N15/10

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US10927385B2

公开(公告)日：2021-02-23

申请号：US16904405

申请日：2020-06-17

Applicant: Inscripta, Inc.

Inventor： Krishna Kannan ,  Miles Gander ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/80 ,  C12N15/10

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US20200071660A1

公开(公告)日：2020-03-05

申请号：US16545097

申请日：2019-08-20

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Isaac Wagner ,  Clint Davis ,  Julia Swavola ,  Phillip Belgrader

IPC: C12N1/20 ,  C12M1/00 ,  G01N35/10 ,  G01N35/00 ,  B01L3/00 ,  C12M1/42 ,  C12M3/04 ,  C12N13/00

Abstract: The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

公开(公告)号：US20220259543A1

公开(公告)日：2022-08-18

申请号：US17734037

申请日：2022-04-30

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky ,  Michael Graige

IPC: C12M1/42 ,  C12M3/00 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US20220195443A1

公开(公告)日：2022-06-23

申请号：US17676210

申请日：2022-02-20

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Tian Tian ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US11365383B1

公开(公告)日：2022-06-21

申请号：US17676746

申请日：2022-02-21

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Amy Hiddessen ,  Andrew Garst ,  Michael Graige ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M3/00 ,  B01L3/00 ,  C12N15/00 ,  C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US20220177826A1

公开(公告)日：2022-06-09

申请号：US17676746

申请日：2022-02-21

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Amy Hiddessen ,  Andrew Garst ,  Michael Graige ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M3/00 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.