公开(公告)号：US20210147858A1

公开(公告)日：2021-05-20

申请号：US16952024

申请日：2020-11-18

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N9/22 ,  C12N1/20

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

公开(公告)号：US20210032613A1

公开(公告)日：2021-02-04

申请号：US17039436

申请日：2020-09-30

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani

IPC: C12N9/22 ,  C12Q1/44 ,  C12N15/85

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US11891609B2

公开(公告)日：2024-02-06

申请号：US17680289

申请日：2022-02-25

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

CPC classification number: C12N15/74 ,  C12N1/20 ,  C12N9/22 ,  C12N15/113 ,  C12N2310/20

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

公开(公告)号：US20220177899A1

公开(公告)日：2022-06-09

申请号：US17680289

申请日：2022-02-25

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome

公开(公告)号：US11274296B2

公开(公告)日：2022-03-15

申请号：US17463956

申请日：2021-09-01

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani ,  Patrick Westfall

IPC: C12N15/10

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20210238586A1

公开(公告)日：2021-08-05

申请号：US17230241

申请日：2021-04-14

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani ,  Patrick Westfall

IPC: C12N15/10

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20210207149A1

公开(公告)日：2021-07-08

申请号：US17199413

申请日：2021-03-11

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Eileen Spindler

IPC: C12N15/62 ,  C12N15/81

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited yeast cells in a cell population when employing nucleic acid-guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US20210071167A1

公开(公告)日：2021-03-11

申请号：US17061143

申请日：2020-10-01

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani

IPC: C12N15/10

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20220195443A1

公开(公告)日：2022-06-23

申请号：US17676210

申请日：2022-02-20

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Tian Tian ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US20220145277A1

公开(公告)日：2022-05-12

申请号：US17522399

申请日：2021-11-09

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Tian Tian ,  Daniel Held

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.