公开(公告)号：US20060158645A1

公开(公告)日：2006-07-20

申请号：US11336588

申请日：2006-01-20

申请人： John Maier ,  Patrick Treado

发明人： John Maier ,  Patrick Treado

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01J3/44 ,  G01J3/2823 ,  G01N21/65 ,  G01N2021/1787

摘要： A method for measuring spatial and spectral information from a sample using computed tomography imaging spectroscopy. An area of the sample is illuminated using an illumination source having substantially monochromatic light. Raman scattered light is directed from said illuminated area of said sample onto a two dimensional grating disperser. Light output, from the two dimensional grating disperser, is directed onto a detector that detects a dispersed image. The dispersed image from the detector is applied to a processing algorithm that generates a plurality of spatially accurate, wavelength resolved images of the sample.

摘要翻译： 一种使用计算机断层摄影成像光谱从样本测量空间和光谱信息的方法。 使用具有基本上单色光的照明源照射样品的区域。 拉曼散射光从所述样品的所述照射区域引导到二维光栅分散器上。 来自二维光栅分散器的光输出被引导到检测器，其检测分散的图像。 来自检测器的分散图像被应用于产生样本的多个空间准确的波长分辨图像的处理算法。

公开(公告)号：US20050052645A1

公开(公告)日：2005-03-10

申请号：US10922006

申请日：2004-08-18

申请人： Shona Stewart ,  John Maier ,  Patrick Treado

发明人： Shona Stewart ,  John Maier ,  Patrick Treado

IPC分类号： C12Q1/04 ,  G01N21/31 ,  G01N21/64 ,  G01N21/65 ,  G01J3/44

CPC分类号： G01N21/6486 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/31 ,  G01N21/33 ,  G01N21/359 ,  G01N21/645 ,  G01N21/6456 ,  G01N21/6458 ,  G01N21/65 ,  G01N2021/1736 ,  G01N2021/6421 ,  G01N2021/6423 ,  G01N2021/656

摘要： Raman scattering of radiation applied to a water sample is used to assess occurrence of a pathogen in the sample. The method is useful for detecting pathogens that are difficult to detect using other methods, such as protozoa. Examples of organisms that can be detected in water samples using these methods include protozoa of the genus Cryptosporidium and the genus Giardia. The methods described herein have important applications, such as for detection of Cryptosporidium organisms in municipal water systems.

公开(公告)号：US09464934B2

公开(公告)日：2016-10-11

申请号：US13347788

申请日：2012-01-11

申请人： Ryan Priore ,  John Maier

发明人： Ryan Priore ,  John Maier

IPC分类号： G01N21/00 ,  G01J3/02 ,  G01J3/44 ,  G01N21/27 ,  G01N21/65 ,  G01J3/28 ,  G01J3/12

CPC分类号： G01J3/02 ,  G01J3/0297 ,  G01J3/28 ,  G01J3/44 ,  G01J2003/1213 ,  G01N21/276 ,  G01N21/65 ,  G01N2201/1293

摘要： The present disclosure provides for a correction filter that may be configured to comprise a predetermined arrangement of thin film layers. This arrangement of thin film layers may be such that it effectively enables a correction filter to generate a predetermined spectral response, wherein said predetermined spectral response is substantially the same as a determined instrument response correction associated with an instrument. The invention of the present disclosure therefore provides for effectively compensating for transmission inefficiencies associated with an instrument without the need for separate reference measurements to determine and correct for instrument response.

摘要翻译： 本公开提供了一种校正滤波器，其可被配置为包括薄膜层的预定排列。 薄膜层的这种布置可以使得其有效地使得校正滤波器能够产生预定的光谱响应，其中所述预定光谱响应与与仪器相关联的确定的仪器响应校正基本相同。 因此，本公开的发明提供了有效地补偿与仪器相关联的传输效率低下，而不需要单独的参考测量来确定和校正仪器响应。

公开(公告)号：US20110208462A1

公开(公告)日：2011-08-25

申请号：US12931836

申请日：2011-02-11

申请人： John Maier ,  Michael Fuhrman

发明人： John Maier ,  Michael Fuhrman

IPC分类号： G06F19/00

CPC分类号： G01J3/02 ,  G01J3/0297 ,  G01J3/44 ,  G01N21/276 ,  G01N21/65 ,  G01N2201/1293

摘要： A system and method for providing an instrument response correction based on transmission efficiency. Test data may be acquired at a first operating point. A look-up table may be searched to determine an instrument response correction. This look-up table may comprise a plurality of instrument response corrections, each instrument response correction corresponding to an intensity response of the sample at an operating point. The instrument response correction may then be applied to correct for environmental factors that may affect transmission efficiency. A system may comprise an illumination source, a collection optics, a tunable filter, a detector, a sensor and a control unit. The detector may detect interacted photons generated by illuminating a sample and generate test data. The sensor may be configured to sense an operating condition and transmit this information to the control unit which is configured to search a look-up table to determine the appropriate instrument response correction.

摘要翻译： 一种基于传输效率提供仪器响应校正的系统和方法。 可以在第一操作点获取测试数据。 可以搜索查找表以确定仪器响应校正。 该查找表可以包括多个仪器响应校正，每个仪器响应校正对应于在操作点处的样品的强度响应。 然后可以应用仪器响应校正来校正可能影响传输效率的环境因素。 系统可以包括照明源，收集光学器件，可调谐滤波器，检测器，传感器和控制单元。 检测器可以检测通过照射样品产生的相互作用的光子并产生测试数据。 传感器可以被配置为感测操作条件并将该信息发送到控制单元，该控制单元被配置为搜索查找表以确定适当的仪器响应校正。

公开(公告)号：US20110007309A1

公开(公告)日：2011-01-13

申请号：US12834462

申请日：2010-07-12

申请人： Shona Stewart ,  John Maier ,  Patrick Treado

发明人： Shona Stewart ,  John Maier ,  Patrick Treado

IPC分类号： G01J3/44

CPC分类号： G01N21/65 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/658 ,  G01N33/1826

摘要： A method for assessing the presence of a pathogenic microorganism in a sample. A sample is illuminated to thereby produce a first plurality of interacted photons which may be scattered, emitted, reflected and/or absorbed by the sample. The first plurality of interacted photons are assessed to thereby generate a Raman data set representative of the sample. This Raman data set is analyzed to thereby determine at least one of: the presence of a pathogenic microorganism in said sample and the absence of a pathogenic microorganism in said sample. The Raman data set may comprise at least one of a Raman spectrum and/or a Raman chemical image representative of the sample. The analysis may comprise comparing said Raman data set to at least one reference Raman data set representative of a known sample. This may be achieved using a chemometric technique.

摘要翻译： 用于评估样品中病原微生物的存在的方法。 照射样品从而产生可被样品散射，发射，反射和/或吸收的第一多个相互作用的光子。 评估第一组多个相互作用的光子，从而产生代表样品的拉曼数据集。 分析该拉曼数据集，从而确定所述样品中病原微生物的存在和所述样品中不存在致病微生物中的至少一种。 拉曼数据集可以包括代表样品的拉曼光谱和/或拉曼化学图像中的至少一个。 分析可以包括将所述拉曼数据集与代表已知样本的至少一个参考拉曼数据集进行比较。 这可以使用化学计量技术来实现。

公开(公告)号：US20100309464A1

公开(公告)日：2010-12-09

申请号：US12619336

申请日：2009-11-16

申请人： Patrick J. Treado ,  Matthew Nelson ,  Jeffrey Cohen ,  John Maier

发明人： Patrick J. Treado ,  Matthew Nelson ,  Jeffrey Cohen ,  John Maier

IPC分类号： G01J3/44

CPC分类号： G01J3/2803 ,  G01J3/0218 ,  G01J3/027 ,  G01J3/0291 ,  G01J3/2889 ,  G01J3/44 ,  G01N21/274 ,  G01N21/65 ,  G01N2201/0216 ,  G01N2201/0697

摘要： The disclosure provides for a system and method for detecting a threat agent. A sample is illuminated to produce photons Raman scattered and emitted by the sample. The Raman scattered photons are collected using time-gated detection without collecting the emitted photons. A Raman spectroscopic data set is generated from said Raman scattered photons wherein said Raman spectroscopic data comprises at least one of a Raman spectrum and a Raman chemical image. The Raman spectroscopic data is assessed to thereby determine the presence or absence of a threat agent in the sample. The sample may be in a target area. The sample may be illuminated using a pulsed laser or an intensity modulated laser. The illumination source may be synchronized with a gating element that enables time-gated detection.

摘要翻译： 本公开提供了用于检测威胁代理的系统和方法。 样品被照亮以产生被样品分散和发射的光子拉曼。 使用时间门控检测收集拉曼散射的光子，而不收集发射的光子。 从所述拉曼散射光子产生拉曼光谱数据集，其中所述拉曼光谱数据包括拉曼光谱和拉曼化学图像中的至少一个。 评估拉曼光谱数据，从而确定样品中威胁剂的存在或不存在。 样本可能在目标区域。 可以使用脉冲激光或强度调制激光来照亮样品。 照明源可以与启用时间门控检测的门控元件同步。

公开(公告)号：US20100033717A1

公开(公告)日：2010-02-11

申请号：US12537671

申请日：2009-08-07

申请人： Janice Panza ,  John Maier

发明人： Janice Panza ,  John Maier

IPC分类号： G01J3/44

CPC分类号： G01J3/44 ,  G01N21/65 ,  G01N2201/129

摘要： A system and method of determining an attribute of a biological tissue sample or a drug delivery device. A sample is illuminated with substantially monochromatic light to thereby generate Raman scattered photons. The Raman scattered photons are assessed to thereby generate a spectroscopic data set wherein said spectroscopic data set comprises at least one of: a Raman spectra and a spatially accurate wavelength resolved image. Tile spectroscopic data set is evaluated to determine at least one of: an attribute of a biological tissue sample and a drug delivery device. In one embodiment, the biological tissue comprises arterial tissue. In another embodiment, the drug delivery device is a drug-eluting stent. In another embodiment, Raman chemical imaging can be used to evaluate a sample and identify at least one of: the tissue, a drug, a drug delivery device, and a matrix associated with a drug delivery device.

摘要翻译： 一种确定生物组织样品或药物递送装置的属性的系统和方法。 用基本单色光照射样品，从而产生拉曼散射光子。 评估拉曼散射光子，从而产生光谱数据集，其中所述光谱数据集包括拉曼光谱和空间上准确的波长分辨图像中的至少一个。 评估平铺光谱数据集以确定生物组织样品和药物递送装置的属性中的至少一个。 在一个实施方案中，生物组织包含动脉组织。 在另一个实施方案中，药物递送装置是药物洗脱支架。 在另一个实施方案中，拉曼化学成像可用于评估样品并鉴定组织，药物，药物递送装置和与药物递送装置相关联的基质中的至少一种。

公开(公告)号：US20080151225A1

公开(公告)日：2008-06-26

申请号：US11351333

申请日：2006-02-09

申请人： Patrick Treado ,  Charles Gardner ,  John Maier

发明人： Patrick Treado ,  Charles Gardner ,  John Maier

IPC分类号： G01N21/00 ,  G01J3/44 ,  G01N21/65

CPC分类号： G01J3/44 ,  G01N21/65

摘要： A system method for depositing a sample of a threat agent is deposited onto a substrate. The threat agent is illuminated via an illumination source along an optical path with a plurality of photons to thereby produce photons transmitted, reflected, emitted or Raman scattered by the threat agent. An optical system collects elastic scatter photons produced by the threat agent and at least one of photons transmitted, reflected, emitted or Raman scattered by the threat agent, wherein said illumination source is located along an optical path, and said substrate is located along a plane wherein the optical path is at an angle other than 90° with respect to the substrate plane. The depth of field of the optical system is extended by passing at least one of the following through a phase mask: elastic scattered photons, and photons transmitted, reflected, emitted or Raman scattered by the threat agent.

摘要翻译： 将用于沉积威胁剂的样品的系统方法沉积在基底上。 通过沿着具有多个光子的光路的照明源照射威胁剂，从而产生被威胁剂散射的透射，反射，发射或拉曼的光子。 光学系统收集由威胁剂产生的弹性散射光子和由威胁剂散射的发射，反射，发射或拉曼的光子中的至少一种，其中所述照明源沿着光路定位，并且所述衬底沿着平面 其中所述光路相对于所述基板平面成90°以外的角度。 通过使以下各项中的至少一个通过相位掩模：弹性散射光子和由威胁剂散发的透射，反射，发射或拉曼的光子来延长光学系统的景深。

公开(公告)号：US20070182959A1

公开(公告)日：2007-08-09

申请号：US10556038

申请日：2005-06-30

申请人： John Maier ,  Patrick Treado ,  David Tuschel ,  Thomas Voigt

发明人： John Maier ,  Patrick Treado ,  David Tuschel ,  Thomas Voigt

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01N21/6456 ,  G01J3/2803 ,  G01J3/32 ,  G01J3/36 ,  G01J3/44 ,  G01N21/359 ,  G01N21/6486 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/656

摘要： The invention relates to methods of dynamic chemical imaging, including methods of cellular imaging. The method comprises illuminating at least a portion of a cell with substantially monochromatic light and assessing Raman-shifted light scattered from the illuminated portion at a plurality of discrete times. The Raman-shifted light can be assessed at a plurality of Raman shift (RS) values at each of the discrete times, and the RS values can be selected to be characteristic of a pre-selected component at each of the discrete times. Multivariate analysis of Raman spectral features of the images thus obtained can yield the location and chemical identity of components in the field of view. This information can be combined or overlaid with other spectral data (e.g., a visible microscopic image) obtained from the field of view.

摘要翻译： 本发明涉及动态化学成像方法，包括细胞成像方法。 该方法包括以基本上单色的光照亮电池的至少一部分，并且在多个离散时间评估从照射部分散射的拉曼偏移光。 可以在每个离散时间处以多个拉曼位移（RS）值评估拉曼移位的光，并且可以将每个离散时间的RS值选择为预选择的分量的特征。 如此获得的图像的拉曼光谱特征的多变量分析可以产生视场中部件的位置和化学特性。 该信息可以与从视野获得的其他光谱数据（例如，可见显微镜图像）组合或覆盖。

公开(公告)号：US20070178067A1

公开(公告)日：2007-08-02

申请号：US11598248

申请日：2006-11-09

申请人： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Lindy McClelland ,  Shona Stewart

发明人： John Maier ,  Joseph Demuth ,  Jeffrey Cohen ,  Lindy McClelland ,  Shona Stewart

IPC分类号： A61K48/00 ,  C12Q1/00 ,  G06F19/00

CPC分类号： G01N33/5005 ,  G01N15/1459 ,  G01N21/65 ,  G01N33/574 ,  G01N33/57407 ,  G01N2021/656

摘要： A method and system of differentially manipulating cells where the cells, suspended in a fluid, are irradiated with substantially monochromatic light. A Raman data set is obtained from the irradiated cells and where the data set is characteristic of a disease status. The data set is assessed to identify diseased cells. A Raman chemical image of the irradiated cells is also obtained. Based on the assessment and the Raman chemical image, the fluid in which the cells are suspended is differentially manipulated. The diseased cells are directed to a first location and other non-diseased cells are directed to a second location as part of the differential manipulation. The diseased cells may be treated with a physical stress, a chemical stress, and a biological stress and then returned to a patient from whom the diseased cells were obtained prior to the irradiation.

摘要翻译： 一种差异操纵细胞的方法和系统，其中悬浮在流体中的细胞用基本上单色的光照射。 从照射的细胞获得拉曼数据集，其中数据集是疾病状态的特征。 评估数据集以鉴定患病细胞。 还获得了照射的细胞的拉曼化学图像。 基于评估和拉曼化学图像，细胞悬浮的流体被差异地操纵。 病变细胞被引导到第一位置，并且其他非病变细胞被引导到第二位置作为差异操纵的一部分。 患病细胞可以用物理应力，化学应力和生物应激治疗，然后返回到在照射之前从其获得患病细胞的患者。