公开(公告)号：US20130003044A1

公开(公告)日：2013-01-03

申请号：US13541171

申请日：2012-07-03

申请人： John Maier ,  Jeffrey Cohen ,  Ryan Priore

发明人： John Maier ,  Jeffrey Cohen ,  Ryan Priore

IPC分类号： G01J3/28 ,  G01J3/44

CPC分类号： G01N21/84 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0224 ,  G01J3/0248 ,  G01J3/027 ,  G01J3/0291 ,  G01J3/10 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/44 ,  G01J3/4406 ,  G01J5/0846 ,  G01J2005/0077 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/65 ,  G01N21/658 ,  G01N2201/129 ,  G02B21/0092 ,  G02B21/14 ,  G02B21/16

摘要： The present disclosure provides for a system and method for assessing chronic exposure of a biological sample, such as a bodily fluid, to an analyte of interest. A biological sample may be illuminated to thereby generate a one or more pluralities of interacted photons. These interacted photons may be detected to thereby generate one or more spectroscopic data sets representative of a biological sample. Spectroscopic data sets generated may be compared to at least one reference data set. Each reference data set may be associated with a known exposure to a known analyte. The present disclosure contemplates that the system and method disclosed herein may be used to analyze exposure of biological samples to at least one analyte over time. Data sets may be obtained at various time intervals to assess changes in a molecular composition as a result of chronic exposure to an analyte.

摘要翻译： 本公开提供了用于评估生物样品（例如体液）到感兴趣分析物的慢性暴露的系统和方法。 可以照射生物样品，从而产生一个或多个相互作用的光子。 可以检测这些相互作用的光子，从而生成代表生物样品的一个或多个光谱数据集。 可以将生成的光谱数据集与至少一个参考数据集进行比较。 每个参考数据集可以与已知分析物的已知暴露相关联。 本公开内容考虑到本文公开的系统和方法可用于分析生物样品随时间的至少一种分析物的暴露。 可以以不同的时间间隔获得数据集，以评估作为慢性暴露于分析物的结果的分子组成的变化。

公开(公告)号：US20130027701A1

公开(公告)日：2013-01-31

申请号：US13347788

申请日：2012-01-11

申请人： Ryan Priore ,  John Maier

发明人： Ryan Priore ,  John Maier

IPC分类号： G01J3/28 ,  G02B5/20 ,  G01J3/45

CPC分类号： G01J3/02 ,  G01J3/0297 ,  G01J3/28 ,  G01J3/44 ,  G01J2003/1213 ,  G01N21/276 ,  G01N21/65 ,  G01N2201/1293

摘要： The present disclosure provides for a correction filter that may be configured to comprise a predetermined arrangement of thin film layers. This arrangement of thin film layers may be such that it effectively enables a correction filter to generate a predetermined spectral response, wherein said predetermined spectral response is substantially the same as a determined instrument response correction associated with an instrument. The invention of the present disclosure therefore provides for effectively compensating for transmission inefficiencies associated with an instrument without the need for separate reference measurements to determine and correct for instrument response.

摘要翻译： 本公开提供了一种校正滤波器，其可被配置为包括薄膜层的预定排列。 薄膜层的这种布置可以使得其有效地使得校正滤波器能够产生预定的光谱响应，其中所述预定光谱响应与与仪器相关联的确定的仪器响应校正基本相同。 因此，本公开的发明提供了有效地补偿与仪器相关联的传输效率低下，而不需要单独的参考测量来确定和校正仪器响应。

公开(公告)号：US09464934B2

公开(公告)日：2016-10-11

申请号：US13347788

申请日：2012-01-11

申请人： Ryan Priore ,  John Maier

发明人： Ryan Priore ,  John Maier

IPC分类号： G01N21/00 ,  G01J3/02 ,  G01J3/44 ,  G01N21/27 ,  G01N21/65 ,  G01J3/28 ,  G01J3/12

CPC分类号： G01J3/02 ,  G01J3/0297 ,  G01J3/28 ,  G01J3/44 ,  G01J2003/1213 ,  G01N21/276 ,  G01N21/65 ,  G01N2201/1293

摘要： The present disclosure provides for a correction filter that may be configured to comprise a predetermined arrangement of thin film layers. This arrangement of thin film layers may be such that it effectively enables a correction filter to generate a predetermined spectral response, wherein said predetermined spectral response is substantially the same as a determined instrument response correction associated with an instrument. The invention of the present disclosure therefore provides for effectively compensating for transmission inefficiencies associated with an instrument without the need for separate reference measurements to determine and correct for instrument response.

摘要翻译： 本公开提供了一种校正滤波器，其可被配置为包括薄膜层的预定排列。 薄膜层的这种布置可以使得其有效地使得校正滤波器能够产生预定的光谱响应，其中所述预定光谱响应与与仪器相关联的确定的仪器响应校正基本相同。 因此，本公开的发明提供了有效地补偿与仪器相关联的传输效率低下，而不需要单独的参考测量来确定和校正仪器响应。

公开(公告)号：US20120078524A1

公开(公告)日：2012-03-29

申请号：US13136366

申请日：2011-07-29

申请人： Shona Stewart ,  Jeffrey Cohen ,  Amy Drauch ,  John Maier

发明人： Shona Stewart ,  Jeffrey Cohen ,  Amy Drauch ,  John Maier

IPC分类号： G01N33/48 ,  G01N21/64 ,  G01J3/44

CPC分类号： A61B5/0059 ,  A61B5/417 ,  G01J3/02 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/10 ,  G01J3/2823 ,  G01J3/32 ,  G01J3/44 ,  G01J2003/2826 ,  G01N21/6458 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/656 ,  G01N2201/1293 ,  G01N2201/1296 ,  G02B21/16 ,  G02B21/365 ,  G06F19/325 ,  G16H50/70

摘要： A system and method for determining a diagnosis of a test biological sample. A system comprising a first illumination source to illuminate a sample, a first detector for generating a fluorescence data set of said sample, a means for determining a region of interest, a second illumination source to illuminate said region of interest, a second detector to generate a Raman data set of said region of interest, and a means for determining a diagnosis of said sample. A method comprising illuminating a sample, generating a fluorescence data set of said sample, and assessing the fluorescence data set to identify a region of interest, illuminating a region of interest, and generating Raman data set. This Raman data set may be assessed to determine a diagnosis of the sample. A diagnosis may include a metabolic state, a clinical outcome, a disease progression, a disease state, and combinations thereof.

摘要翻译： 用于确定测试生物样品的诊断的系统和方法。 一种系统，包括用于照亮样品的第一照明源，用于产生所述样品的荧光数据集的第一检测器，用于确定感兴趣区域的装置，用于照亮所述感兴趣区域的第二照明源，产生 所述感兴趣区域的拉曼数据集，以及用于确定所述样本的诊断的装置。 一种方法，包括照亮样品，产生所述样品的荧光数据集，以及评估所述荧光数据集以识别感兴趣区域，照亮感兴趣区域，以及产生拉曼数据集。 可以评估该拉曼数据集以确定样品的诊断。 诊断可以包括代谢状态，临床结果，疾病进展，疾病状态及其组合。

公开(公告)号：US07956996B2

公开(公告)日：2011-06-07

申请号：US12206500

申请日：2008-09-08

申请人： John Maier ,  Jeffrey Cohen ,  Shona Stewart

发明人： John Maier ,  Jeffrey Cohen ,  Shona Stewart

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01J3/02 ,  A61B5/0059 ,  A61B5/417 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/28 ,  G01J3/2823 ,  G01J3/36 ,  G01J3/44 ,  G01J2003/2826 ,  G01N21/65 ,  G01N33/57415 ,  G01N2021/656 ,  G01N2201/1293 ,  G01N2201/1296 ,  Y02A90/26

摘要： A system and method to provide a diagnosis of the breast disease state of a test breast sample. A database containing a plurality of reference Raman data sets is provided where each reference Raman data set has an associated known breast sample and an associated known breast disease state. A test breast sample is irradiated with substantially monochromatic light to generate scattered photons resulting in a test Raman data set. The test Raman data set is compared to the plurality of reference Raman data sets using a chemometric technique. Based on the comparison, a diagnosis of a breast disease state of the test breast sample is provided. The breast disease state includes invasive ductal carcinoma or invasive lobular carcinoma disease state.

摘要翻译： 一种用于提供测试乳房样品的乳腺疾病状态诊断的系统和方法。 提供了包含多个参考拉曼数据集的数据库，其中每个参考拉曼数据集具有相关联的已知乳房样本和相关联的已知乳腺疾病状态。 用基本上单色的光照射测试乳样品以产生散射的光子，得到测试拉曼数据集。 使用化学计量技术将测试拉曼数据集与多个参考拉曼数据集进行比较。 基于比较，提供了测试乳房样品的乳腺疾病状态的诊断。 乳腺疾病状态包括浸润性导管癌或侵袭性小叶癌疾病状态。

公开(公告)号：US07570356B2

公开(公告)日：2009-08-04

申请号：US11650378

申请日：2007-01-05

申请人： Janice L. Panza ,  John Maier ,  Jason Neiss

发明人： Janice L. Panza ,  John Maier ,  Jason Neiss

IPC分类号： G01J3/44 ,  G01J3/00

CPC分类号： G01J3/44 ,  G01J3/28 ,  G01J3/36 ,  G01N15/1475 ,  G01N21/65 ,  G01N2015/1006 ,  G01N2015/1488 ,  G01N2021/656

摘要： A system and method to distinguish normal cells from apoptotic cells. A pre-determined vector space is selected where the vector space mathematically describes a first plurality of reference Raman data sets for normal cells and a second plurality of reference Raman data sets for apoptotic cells. A sample is irradiated with substantially monochromatic light generating a target Raman data set based on scattered photons. The target Raman data set is transformed into a vector space defined by the pre-determined vector space. A distribution of transformed data is analyzed in the pre-determined vector space. Based on the analysis, the sample is classified as containing normal cells, apoptotic cells, and a combination of normal and apoptotic cells. The sample includes the step of treating the sample with a pharmaceutical agent prior to irradiating the sample. Based on the classification, the therapeutic efficiency of the pharmaceutical agent is assessed.

摘要翻译： 将正常细胞与凋亡细胞区分开的系统和方法。 选择预定矢量空间，其中矢量空间在数学上描述用于正常细胞的第一多个参考拉曼数据集和用于凋亡细胞的第二多个参考拉曼数据集。 用基本上单色的光照射样品，产生基于散射光子的目标拉曼数据集。 目标拉曼数据集被转换成由预定向量空间定义的向量空间。 在预定向量空间中分析变换数据的分布。 基于分析，样品被分类为含有正常细胞，凋亡细胞，以及正常细胞和凋亡细胞的组合。 样品包括在照射样品之前用药剂处理样品的步骤。 根据分类，评估药剂的治疗效果。

公开(公告)号：US07557915B2

公开(公告)日：2009-07-07

申请号：US11585535

申请日：2006-10-24

申请人： John Maier ,  Patrick Treado ,  Matthew Nelson ,  Lindy McClelland ,  Shona Stewart

发明人： John Maier ,  Patrick Treado ,  Matthew Nelson ,  Lindy McClelland ,  Shona Stewart

IPC分类号： G01J3/44

CPC分类号： G01N21/65 ,  G01J3/28 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/274 ,  G01N2021/656

摘要： A system and method to automatically obtain spectra for samples. The method involves a two phase process including a photobleaching phase and a spectral acquisition phase. In the photobleaching phase, a series of spectral data sets of a sample are collected. A relative difference is determined between the background of subsequent spectral data sets is determined and compared to a predetermined threshold value. If threshold difference is less than the relative difference between the background of subsequent spectral data sets, the steps of collecting a series of spectra data sets is automatically repeated. In the spectrum acquisition phase, a series of Raman data sets of the sample are collected until a target SNR is obtained.

摘要翻译： 自动获取样品光谱的系统和方法。 该方法涉及包括光漂白相和光谱采集阶段的两相过程。 在漂白漂白阶段，收集样品的一系列光谱数据集。 在确定后续光谱数据集的背景并将其与预定阈值进行比较之间确定相对差。 如果阈值差小于随后的光谱数据集的背景之间的相对差，则自动重复收集一系列光谱数据集的步骤。 在频谱采集阶段，收集样本的一系列拉曼数据集，直到获得目标SNR。

公开(公告)号：US20090033930A1

公开(公告)日：2009-02-05

申请号：US12248344

申请日：2008-10-09

申请人： John Maier ,  Jeffrey Cohen

发明人： John Maier ,  Jeffrey Cohen

IPC分类号： G01N21/65

CPC分类号： G01N21/65 ,  G01J3/02 ,  G01J3/0227 ,  G01J3/0291 ,  G01J3/44 ,  G01N21/658 ,  G01N2021/656 ,  G01N2201/129

摘要： A spectroscopic method and system to identify a biofilm of a microorganism. A sample containing a sample microorganism is irradiated with substantially monochromatic radiation. A Raman data set is obtained based on radiation scattered from the irradiated sample. A database is searched in accordance with the Raman data set in order to identify a known Raman data set from the database. The database contains a plurality of known Raman data sets where each known Raman data set is associated with a known sessile form of a corresponding known microorganism. A sessile form of the sample microorganism is identified based on the known Raman data set identified by the searching.

摘要翻译： 鉴定微生物生物膜的光谱方法和系统。 用基本上单色的辐射照射含有样品微生物的样品。 基于从照射样品散射的辐射获得拉曼数据集。 根据拉曼数据集搜索数据库，以便从数据库中识别已知的拉曼数据集。 数据库包含多个已知的拉曼数据集，其中每个已知的拉曼数据集与相应的已知微生物的已知无形形式相关联。 基于通过搜索识别的已知拉曼数据集来鉴定样品微生物的固定形式。

公开(公告)号：US20080151224A1

公开(公告)日：2008-06-26

申请号：US11351280

申请日：2006-02-09

申请人： Patrick Treado ,  Charles Gardner ,  John Maier

发明人： Patrick Treado ,  Charles Gardner ,  John Maier

IPC分类号： G01N21/00 ,  G01J3/44 ,  G01N21/65

CPC分类号： G01J3/44 ,  G01N21/65

摘要： A system and method for depositing a sample of a threat agent onto a substrate. A single illumination source illuminates the threat agent deposited on the substrate with a plurality of photons to thereby produce elastic scattered photons and Raman scattered photons. The threat agent on the substrate is identified. The system and method operate in a trigger mode that detects the presence or absence of the threat agent, and an identification mode that identifies the threat agent.

摘要翻译： 一种用于将威胁剂样品沉积到基底上的系统和方法。 单个照明源用多个光子照射沉积在基板上的威胁剂，从而产生弹性散射的光子和拉曼散射的光子。 识别底物上的威胁剂。 系统和方法在检测威胁代理的存在或不存在的触发模式下操作，以及识别威胁代理的识别模式。

公开(公告)号：US20080034833A1

公开(公告)日：2008-02-14

申请号：US11831088

申请日：2007-07-31

申请人： John Maier

发明人： John Maier

IPC分类号： G12B13/00 ,  G01N1/00

CPC分类号： G01J3/44 ,  G01J3/02 ,  G01J3/0297 ,  G01N21/276 ,  G01N21/65

摘要： A system and method for correction of instrument response of an optical spectroscopy instrument using a Raman standard sample supplied by NIST (National Institute of Standards and Technology). The smoother side of the NIST sample is placed facing a light collection optics in the spectroscopy instrument, whereas the non-smooth or rough side remains away from the light collection optics, but in contact with a platform or sample placement surface in the spectroscopy instrument. An instrument response function is determined with the NIST sample so placed. Thereafter, spectra or spectral images of target samples obtained using the spectroscopy instrument are divided by the instrument response function to correct for imperfections in the response of the optical spectroscopy instrument. The target sample spectra may be non-Raman spectra. The optical spectroscopy instrument may be a gratings-based or a tunable filter based chemical imaging system.

摘要翻译： 用于校正由NIST（国家标准与技术研究所）提供的拉曼标准样品的光谱仪器的仪器响应的系统和方法。 NIST样品的平滑侧放置在光谱仪器中的光收集光学器件上，而非光滑或粗糙的一侧保留远离光收集光学器件，但与光谱仪器中的平台或样品放置表面接触。 仪器响应功能由放置的NIST样品确定。 此后，使用光谱仪器获得的目标样品的光谱或光谱图像除以仪器响应函数，以校正光谱仪器响应中的缺陷。 目标样品光谱可以是非拉曼光谱。 光谱仪器可以是基于光栅或基于可调滤波器的化学成像系统。