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公开(公告)号：US07268271B2
公开(公告)日：2007-09-11
申请号：US10199609
申请日：2002-07-19
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8287 , Y02A40/146
摘要： The invention provides novel uses for isolated LEC1 nucleic acids and their encoded proteins. The proteins are transcriptional activators. The invention further provides expression cassettes, transformed host cells, and transgenic plants and plant parts.
摘要翻译： 本发明提供了分离的LEC1核酸及其编码蛋白的新用途。 蛋白质是转录激活因子。 本发明进一步提供表达盒，转化的宿主细胞和转基因植物和植物部分。
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公开(公告)号：US07256280B2
公开(公告)日：2007-08-14
申请号：US09993808
申请日：2001-11-06
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated nucleic acids and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content, cell cycle progression, cell number and/or composition of plants.
摘要翻译： 本发明提供分离的核酸及其编码的参与细胞周期调节的蛋白质。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了涉及改变细胞周期蛋白质含量，细胞周期进程，细胞数量和/或植物组成的方法和组合物。
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公开(公告)号：US08704041B2
公开(公告)日：2014-04-22
申请号：US12982013
申请日：2010-12-30
申请人： William J. Gordon-Kamm , Keith S. Lowe , David J. Peterson , Christopher J. Scelonge , Grace M. St. Clair , Bing-Bing Wang
发明人： William J. Gordon-Kamm , Keith S. Lowe , David J. Peterson , Christopher J. Scelonge , Grace M. St. Clair , Bing-Bing Wang
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8213
摘要： A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.
摘要翻译： 提供了各种方法和组合物，包括用于靶向修饰细胞或生物体中特定靶位点的方法和组合物，用于整合感兴趣的多核苷酸的方法，评估启动子活性的方法，直接选择转化的生物体，最小化或消除产生的表达 从随机整合到生物体如植物的基因组中，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，使基因沉默，并鉴定和/或表征转录调节区。 该方法涉及将细胞增殖因子和双链断裂诱导酶引入生物体中。
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公开(公告)号：US20110258741A1
公开(公告)日：2011-10-20
申请号：US13168133
申请日：2011-06-24
申请人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
发明人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。
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公开(公告)号：US20080201806A1
公开(公告)日：2008-08-21
申请号：US11940371
申请日：2007-11-15
申请人： William J. Gordon-Kamm , Keith S. Lowe , Christopher J. Scelonge , Carl R. Simmons , Aurelie Morin , Lawrence R. Stiner
发明人： William J. Gordon-Kamm , Keith S. Lowe , Christopher J. Scelonge , Carl R. Simmons , Aurelie Morin , Lawrence R. Stiner
CPC分类号： C12N15/8257 , C12N15/8238
摘要： Compositions and methods relating to the use of tetracycline repressor in plants are provided. Compositions include a polynucleotide modified for expression in a plant, wherein the polynucleotide encodes a tetracycline repressor protein, as well as constructs, vectors, cells, plants and seeds comprising the polynucleotide, an/or produced by the methods. Also provided are methods to provide tetracycline repressor to a cell, and to regulate expression of a polynucleotide of interest in a cell, including a plant cell.
摘要翻译： 提供了在植物中使用四环素阻遏物的组合物和方法。 组合物包括修饰用于在植物中表达的多核苷酸，其中所述多核苷酸编码四环素阻遏物蛋白，以及构建体，载体，细胞，植物和包含所述多核苷酸的/或由所述方法产生的种子。 还提供了向细胞提供四环素阻遏物并调节目标多核苷酸在包括植物细胞的细胞中的表达的方法。
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公开(公告)号：US06800791B1
公开(公告)日：2004-10-05
申请号：US09570319
申请日：2000-05-12
申请人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
发明人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
IPC分类号： A01H500
CPC分类号： C12N15/62 , C07H21/04 , C07K2319/09 , C07K2319/10 , C07K2319/61 , C07K2319/80 , C12N15/8205
摘要： The invention relates to the use of a modified Agrobacterium to deliver proteins directly to plant cells. Proteins of interest are delivered to the plant host in the form of a fusion protein with the Agrobacterium virulence protein VirF. Nucleotide sequences encoding such fusion proteins of VirF and a protein of interest are provided. Also provided are bacteria modified to comprise such fusion proteins of VirF and a protein of interest. Methods of introducing such fusion proteins into a plant host are provided. The invention finds use in facilitating plant transformation and particularly in the bio-engineering of desirable traits into crop plants.
摘要翻译： 本发明涉及使用改良的农杆菌将蛋白质直接递送至植物细胞。 感兴趣的蛋白质以与农杆菌毒力蛋白VirF融合蛋白的形式递送至植物宿主。 提供编码VirF和感兴趣蛋白质的融合蛋白的核苷酸序列。 还提供了细菌修饰以包含VirF和感兴趣的蛋白质的这种融合蛋白。 提供将这种融合蛋白引入植物宿主的方法。本发明用于促进植物转化，特别是在对作物植物期望的性状的生物工程中。
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公开(公告)号：US06441151B1
公开(公告)日：2002-08-27
申请号：US09395674
申请日：1999-09-14
IPC分类号： C07H2104
CPC分类号： C07K14/415 , C12N15/8279
摘要： Compositions and methods for modulating cell cycle and cell proliferation are provided. Additionally the compositions find use in enhancing disease resistance and increasing transformation efficiency in plants. The method involves transforming a plant with a sense or antisense prohibitin sequence. The prohibitin sequence acts to regulate cell division in the plant cell. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.
摘要翻译： 提供了调节细胞周期和细胞增殖的组合物和方法。 此外，组合物可用于增强抗病性并提高植物的转化效率。 该方法涉及用有义或反义禁忌蛋白序列转化植物。 禁食序列用于调节植物细胞中的细胞分裂。 还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。
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公开(公告)号：US07569746B2
公开(公告)日：2009-08-04
申请号：US10913202
申请日：2004-08-06
申请人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
发明人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
CPC分类号： C12N15/62 , C07H21/04 , C07K2319/09 , C07K2319/10 , C07K2319/61 , C07K2319/80 , C12N15/8205
摘要： The invention relates to the use of a modified Agrobacterium to deliver proteins directly to plant cells. Proteins of interest are delivered to the plant host in the form of a fusion protein with the Agrobacterium virulence protein VirF. Nucleotide sequences encoding such fusion proteins of VirF and a protein of interest are provided. Also provided are bacteria modified to comprise such fusion proteins of VirF and a protein of interest. Methods of introducing such fusion proteins into a plant host are provided.The invention finds use in facilitating plant transformation and particularly in the bio-engineering of desirable traits into crop plants.
摘要翻译： 本发明涉及使用改良的农杆菌将蛋白质直接递送至植物细胞。 感兴趣的蛋白质以与农杆菌毒力蛋白VirF融合蛋白的形式递送至植物宿主。 提供编码VirF和感兴趣蛋白质的融合蛋白的核苷酸序列。 还提供了细菌修饰以包含VirF和感兴趣的蛋白质的这种融合蛋白。 提供了将这种融合蛋白引入植物宿主的方法。 本发明用于促进植物转化，特别是在对作物植物期望的性状的生物工程中。
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公开(公告)号：US20090142829A1
公开(公告)日：2009-06-04
申请号：US12256721
申请日：2008-10-23
申请人： Kimberly F. Glassman , William J. Gordon-Kamm , Anthony J. Kinney , Keith S. Lowe , Scott E. Nichols , Kevin L. Stecca
发明人： Kimberly F. Glassman , William J. Gordon-Kamm , Anthony J. Kinney , Keith S. Lowe , Scott E. Nichols , Kevin L. Stecca
CPC分类号： C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/825 , C12N15/8251
摘要： Recombinant constructs useful for reducing the expression of endogenous mRNA and any substantially similar endogenous mRNA are disclosed. In particular, a recombinant construct comprising, inter alia, a suitable nucleic acid sequence and its reverse complement can be used to alter the expression of any homologous, endogenous RNA (i.e., the target RNA) which is in proximity to this suitable nucleic acid sequence.
摘要翻译： 公开了可用于减少内源性mRNA的表达和任何基本上相似的内源mRNA的重组构建体。 特别地，包含尤其是合适的核酸序列及其反向互补序列的重组构建体可用于改变任何同源的内源RNA（即靶RNA）的表达，其接近该合适的核酸序列 。
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公开(公告)号：US07256322B2
公开(公告)日：2007-08-14
申请号：US10744572
申请日：2003-12-23
申请人： Keith S. Lowe , Rebecca E. Cahoon , Christopher J. Scelonge , Yumin Tao , William J. Gordon-Kamm , Wesley B. Bruce , Lisa J. Newman
发明人： Keith S. Lowe , Rebecca E. Cahoon , Christopher J. Scelonge , Yumin Tao , William J. Gordon-Kamm , Wesley B. Bruce , Lisa J. Newman
CPC分类号： C12N15/8207 , C07K14/415 , C12N15/8201 , C12N15/821 , C12N15/8241 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。
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