公开(公告)号：US10041904B2

公开(公告)日：2018-08-07

申请号：US15704695

申请日：2017-09-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James Ball ,  Brian Reed

IPC: B08B3/04 ,  G01N27/414 ,  B08B3/08 ,  C11D7/34 ,  C11D7/50 ,  B08B9/08 ,  C11D3/34 ,  C11D3/16 ,  C11D3/43

Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.

公开(公告)号：US20170206313A1

公开(公告)日：2017-07-20

申请号：US15359773

申请日：2016-11-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian Reed

IPC: G06F19/22 ,  C12Q1/68

CPC classification number: G16B30/00 ,  C12Q1/6874

Abstract: Systems and method for identifying duplicate reads can receive first and second reads, determine if the first and second reads have a same start and end position, determine a binary flow difference, and identify the second read as a duplicate of the first read when the binary flow difference exceeds a threshold.

公开(公告)号：US20170014784A1

公开(公告)日：2017-01-19

申请号：US15282283

申请日：2016-09-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan SCHULTZ ,  John Nobile ,  Brian Reed ,  Prasanna Thwar ,  Todd Roswech ,  John Andrew Sheridan

IPC: B01F3/08 ,  B01L7/00 ,  B01F13/00 ,  B04B11/02 ,  B04B7/02 ,  B04B5/04 ,  C12Q1/68 ,  B01L3/00

CPC classification number: B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F15/00 ,  B01F2215/0037 ,  B01L3/5021 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/06 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2400/0409 ,  B04B5/0414 ,  B04B7/02 ,  B04B11/02 ,  B04B2007/025 ,  C12M33/10 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2523/32 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

Abstract translation: 提供了一种自动化模板珠制备系统，包括膜基乳液生成子系统，热板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式将逆向乳液的连续流供应到热子系统。 热子系统可以处理通过其中的反相乳液。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。