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Patent Agency Ranking
公开(公告)号：US20170014784A1
公开(公告)日：2017-01-19
申请号：US15282283
申请日：2016-09-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan SCHULTZ , John Nobile , Brian Reed , Prasanna Thwar , Todd Roswech , John Andrew Sheridan
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
Abstract translation: 提供了一种自动化模板珠制备系统，包括膜基乳液生成子系统，热板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式将逆向乳液的连续流供应到热子系统。 热子系统可以处理通过其中的反相乳液。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。
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公开(公告)号：US09458485B2
公开(公告)日：2016-10-04
申请号：US14799259
申请日：2015-07-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , John Nobile , Brian Reed , Prasanna Krishnan Thwar , Todd Roswech , John Andrew Sheridan
IPC: C12P19/34 , C12M1/26 , B01F15/00 , B04B11/02 , B01F5/04 , B01F13/00 , B01L3/00 , B01L7/00 , B04B5/04 , B04B7/02 , B01F3/08 , C12Q1/68
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
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公开(公告)号：US09901887B2
公开(公告)日：2018-02-27
申请号：US15282283
申请日：2016-09-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , John Nobile , Brian Reed , Prasanna Thwar , Todd Roswech , John Andrew Sheridan
IPC: C12M1/00 , C12M3/00 , C12M1/34 , B01F3/08 , C12P19/34 , C12M1/26 , B01F15/00 , B04B11/02 , B01F5/04 , B01F13/00 , B01L3/00 , B01L7/00 , B04B5/04 , B04B7/02 , C12Q1/68
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
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公开(公告)号：US20150314294A1
公开(公告)日：2015-11-05
申请号：US14799259
申请日：2015-07-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan SCHULTZ , John Nobile , Brian Reed , Prasanna Krishnan Thwar , Todd Roswech , John Andrew Sheridan
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
Abstract translation: 提供了一种自动化模板珠制备系统，包括膜基乳液生成子系统，热板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式将逆向乳液的连续流供应到热子系统。 热子系统可以处理通过其中的反相乳液。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。
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公开(公告)号：US09150917B2
公开(公告)日：2015-10-06
申请号：US14057450
申请日：2013-10-18
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Todd Rearick , Jessica Lynn Reed , Jason Gioia , Devin Dressman , Nicholas Hapshe , Brian Reed , John Andrew Sheridan
IPC: C12Q1/68 , B01L3/00 , G01N33/50 , G01N33/543
CPC classification number: C12Q1/6874 , B01L3/5085 , B01L2200/0642 , B01L2200/0668 , B01L2300/0819 , B01L2300/0893 , C12Q1/6837 , G01N33/50 , G01N33/543
Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.
Abstract translation: 提供了用于将样品沉积到表面上的方法，组合物，系统，装置和试剂盒。 样品可以包括一个或多个颗粒，并且该表面可以包括一个或多个反应室。 在一些实施方案中，沉积可以包括使用伴随颗粒与样品颗粒的组合。
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