公开(公告)号：US20150307929A1

公开(公告)日：2015-10-29

申请号：US14795774

申请日：2015-07-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John LEAMON ,  Mark Andersen ,  Michael Thornton

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US20150307920A1

公开(公告)日：2015-10-29

申请号：US14796696

申请日：2015-07-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John LEAMON ,  Mark Andersen ,  Michael Thornton

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US20240230501A1

公开(公告)日：2024-07-11

申请号：US18612831

申请日：2024-03-21

Applicant: Life Technologies Corporation

Inventor： Mark Andersen ,  Haopeng Wang ,  Michael Pallas

IPC: G01N15/04 ,  C12Q1/6816 ,  C12Q1/686

CPC classification number: G01N15/04 ,  C12Q1/6816 ,  C12Q1/686

Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.

公开(公告)号：US10100354B2

公开(公告)日：2018-10-16

申请号：US14795826

申请日：2015-07-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6855 ,  C12Q1/6879 ,  C12Q1/6883

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.

公开(公告)号：US10017811B2

公开(公告)日：2018-07-10

申请号：US14518904

申请日：2014-10-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC: C07H21/04 ,  C07H21/02 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12N15/10 ,  C12Q1/6886 ,  C12Q1/6874 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US09951380B2

公开(公告)日：2018-04-24

申请号：US14795774

申请日：2015-07-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John Leamon ,  Mark Andersen ,  Michael Thornton

IPC: C12Q1/68 ,  C12N15/10 ,  C07H21/04

CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US20160046979A1

公开(公告)日：2016-02-18

申请号：US14795826

申请日：2015-07-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John LEAMON ,  Mark Andersen ,  Michael Thornton

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6855 ,  C12Q1/6879 ,  C12Q1/6883 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.