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    SYSTEM AND METHOD FOR DETERMINING COPIES-PER-UNIT-VOLUME USING PCR AND FLOW CONTROL OF DROPLETS
    21.
    发明申请
    SYSTEM AND METHOD FOR DETERMINING COPIES-PER-UNIT-VOLUME USING PCR AND FLOW CONTROL OF DROPLETS 审中-公开
    使用PCR和流量控制法确定复制单位体积的系统和方法

    公开(公告)号：US20160208342A1

    公开(公告)日：2016-07-21

    申请号：US15080801

    申请日：2016-03-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael C. Pallas ,  Marcin Sikora ,  Casey R. McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

    IPC: C12Q1/68 ,  G01N21/64

    CPC classification number: C12Q1/6886 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N21/6486 ,  C12Q2537/16 ,  C12Q2563/143 ,  C12Q2563/159 ,  C12Q2565/626

    Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

    Abstract translation: 提供了用于定量样品中靶核酸的方法和系统。 该方法包括形成多个离散的样本部分。 多个离散样品部分中的每一个包含样品的一部分和反应混合物。 该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。 至少一个离散加工的样品部分含有核酸扩增反应产物。 从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。 该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。 估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。

    Flat-Field Imaging System and Methods of Use
    24.
    发明公开
    Flat-Field Imaging System and Methods of Use 审中-公开

    公开(公告)号：US20240230501A1

    公开(公告)日：2024-07-11

    申请号：US18612831

    申请日：2024-03-21

    Applicant: Life Technologies Corporation

    Inventor： Mark Andersen ,  Haopeng Wang ,  Michael Pallas

    IPC: G01N15/04 ,  C12Q1/6816 ,  C12Q1/686

    CPC classification number: G01N15/04 ,  C12Q1/6816 ,  C12Q1/686

    Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.

    Methods and compositions for multiplex PCR
    25.
    发明授权
    Methods and compositions for multiplex PCR 有权

    公开(公告)号：US10100354B2

    公开(公告)日：2018-10-16

    申请号：US14795826

    申请日：2015-07-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John Leamon ,  Mark Andersen ,  Michael Thornton

    IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6855 ,  C12Q1/6879 ,  C12Q1/6883

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    28.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开

    公开(公告)号：US20160046979A1

    公开(公告)日：2016-02-18

    申请号：US14795826

    申请日：2015-07-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John LEAMON ,  Mark Andersen ,  Michael Thornton

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6855 ,  C12Q1/6879 ,  C12Q1/6883 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.

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