公开(公告)号：US20220248686A1

公开(公告)日：2022-08-11

申请号：US17584677

申请日：2022-01-26

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Danqi Chen ,  Todd A. Ciche ,  Arlene R. Howe ,  Jennifer L. Lutke ,  Barbana E. Wiggins ,  Yuanji Zhang

IPC: A01N63/25 ,  C12N15/70 ,  C12Q1/6895 ,  C07K14/195 ,  C12N15/82

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3 pesticidal proteins are also provided. Also disclosed are methods and compositions to improve the insecticidal activity of a pesticidal protein against an insect pest species. Further disclosed are method and compositions to reduce expression of a pesticidal protein in the reproductive tissues of a transgenic plant.

公开(公告)号：US20210246464A1

公开(公告)日：2021-08-12

申请号：US17242049

申请日：2021-04-27

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Uma R. Kesanapalli ,  Jennifer L. Lutke

IPC: C12N15/82 ,  C12Q1/6895 ,  A01N63/50 ,  A01N37/46 ,  G01N33/53

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL pesticidal proteins are also provided.

公开(公告)号：US20200308632A1

公开(公告)日：2020-10-01

申请号：US16642339

申请日：2018-08-29

Applicant: Monsanto Technology LLC

Inventor： Adil Anjem ,  Amy L. Caruano-Yzermans ,  Todd A. Ciche ,  Julia L. Stevens ,  Keith H. Turner

IPC: C12Q1/689 ,  C12Q1/18 ,  A01N63/27 ,  A01N63/23 ,  A01N63/36

Abstract: Methods useful in detecting viable microbes in an agricultural composition are provided. Certain embodiments relate to methods in which a sample of an agricultural composition is obtained, and an amount of at least one pre-rRNA from at least one microbe is detected. The detected pre-rRNA from an agricultural sample may be compared with the amount of pre-rRNA from a control sample to determine the presence of a viable microbe.

公开(公告)号：US20200229445A1

公开(公告)日：2020-07-23

申请号：US16748392

申请日：2020-01-21

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A Chay ,  Danqi Chen ,  Todd A. Ciche ,  Arlene R. Howe ,  Jennifer L. Lutke ,  Barbara E. Wiggins ,  Yuanji Zhang

IPC: A01N63/25 ,  C12N15/70 ,  C12N15/82 ,  C12Q1/6895 ,  C07K14/195

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3 pesticidal proteins are also provided. Also disclosed are methods and compositions to improve the insecticidal activity of a pesticidal protein against an insect pest species. Further disclosed are method and compositions to reduce expression of a pesticidal protein in the reproductive tissues of a transgenic plant.

公开(公告)号：US12234471B2

公开(公告)日：2025-02-25

申请号：US18506855

申请日：2023-11-10

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Uma R. Kesanapalli ,  Jennifer L. Lutke

IPC: C12N15/82 ,  A01N63/50 ,  C12Q1/6895

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL pesticidal proteins are also provided.

公开(公告)号：US20240301441A1

公开(公告)日：2024-09-12

申请号：US18635866

申请日：2024-04-15

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe

IPC: C12N15/82 ,  A01N63/50 ,  C07K14/24 ,  C07K14/325

CPC classification number: C12N15/8286 ,  A01N63/50 ,  C07K14/24 ,  C07K14/325

Abstract: A pesticidal protein class of PirA, PirB, and PirAB fusion proteins exhibiting toxic activity against Coleopteran, Lepidopteran, and Hemipteran pest species is disclosed. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the PirA, PirB, and PirAB fusion proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran, Lepidopteran, and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the PirA, PirB, and PirAB fusion proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, and Hemipteran species pests using the PirA, PirB, and PirAB fusion proteins are also provided.

公开(公告)号：US11807864B2

公开(公告)日：2023-11-07

申请号：US18151119

申请日：2023-01-06

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Uma R. Kesanapalli ,  Jennifer L Lutke

IPC: C12N15/82 ,  C12Q1/6895 ,  A01N37/46 ,  A01N63/50 ,  G01N33/53

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/50 ,  C12Q1/6895 ,  C12Q2600/13 ,  G01N33/5308 ,  Y02A40/146 ,  A01N63/50 ,  A01N63/25

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL pesticidal proteins are also provided.

公开(公告)号：US20230250447A1

公开(公告)日：2023-08-10

申请号：US17934287

申请日：2022-09-22

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe

IPC: C12N15/82 ,  C07K14/325 ,  G01N33/569 ,  G01N33/00 ,  A01N63/10 ,  A01N63/50

CPC classification number: C12N15/8286 ,  C07K14/325 ,  G01N33/56961 ,  G01N33/0098 ,  A01N63/10 ,  A01N63/50

Abstract: A pesticidal protein class of PirA, PirB, and PirAB fusion proteins exhibiting toxic activity against Coleopteran, Lepidopteran, and Hemipteran pest species is disclosed. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the PirA, PirB, and PirAB fusion proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran, Lepidopteran, and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the PirA, PirB, and PirAB fusion proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, and Hemipteran species pests using the PirA, PirB, and PirAB fusion proteins are also provided.

公开(公告)号：US11673922B2

公开(公告)日：2023-06-13

申请号：US17561332

申请日：2021-12-23

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Todd A. Ciche ,  Arlene R. Howe ,  Stephanie C. Waldheuser ,  Kimberly M. Wegener

IPC: C07K14/32 ,  C12N15/82 ,  A01P7/04 ,  A01N63/50 ,  C07K14/325

CPC classification number: C07K14/325 ,  A01N63/50 ,  A01P7/04 ,  C12N15/8286

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC13085 and TIC13087. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC13085 and TIC13087 pesticidal proteins are also provided.

公开(公告)号：US20220235369A1

公开(公告)日：2022-07-28

申请号：US17575318

申请日：2022-01-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flainski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  A01N37/46 ,  C07K14/195 ,  C07K14/32 ,  A01N63/50

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.