Polypeptides With Lipase Activity and Polynucleotides Encoding Same
    24.
    发明申请
    Polypeptides With Lipase Activity and Polynucleotides Encoding Same 有权
    具有脂肪酶活性的多肽和编码相同的多核苷酸

    公开(公告)号:US20160010038A1

    公开(公告)日:2016-01-14

    申请号:US14770006

    申请日:2014-03-19

    申请人: NOVOZYMES A/S

    IPC分类号: C11D3/386 C12P13/00 C12N9/20

    摘要: The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 3 or the full-length complement of (i); (c) a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 3; (d) a polypeptide which is a variant of SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and (e) a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

    摘要翻译: 本发明涉及具有脂肪酶活性的分离的多肽,其选自:(a)多肽,其具有至少75%,至少80%,至少85%,至少90%,至少91%,在 与SEQ ID NO:4的至少92%,至少93%,至少94%,至少95%,至少96%,至少97%,至少98%,至少99%或100%的序列同一性 ; (b)由多核苷酸编码的多肽,其在低严格条件,中等严格条件,中等严格条件,高严格条件或非常高严格条件下与(i)SEQ ID NO:3或全长补体 的(i); (c)由多核苷酸编码的多肽,所述多核苷酸具有至少75%,至少80%,至少85%,至少90%,至少91%,至少92%,至少93%,至少94% 与SEQ ID NO:3的至少95%,至少96%,至少97%,至少98%,至少99%或100%的序列同一性; (d)在一个或多个(例如数个)位置上包含取代,缺失和/或插入的SEQ ID NO:4的变体的多肽; 和(e)作为(a),(b),(c)或(d)的多肽的片段的多肽。 本发明还涉及编码多肽的多核苷酸; 核酸构建体,载体和包含多核苷酸的宿主细胞; 和使用多肽的方法。

    Compositions and processes for treatment with lipases

    公开(公告)号:US10731110B2

    公开(公告)日:2020-08-04

    申请号:US15104265

    申请日:2014-12-18

    申请人: Novozymes A/S

    摘要: The present invention relates to a composition comprising: (a) at least one surfactant, at least one surfactant system, at least one soap, or any mixtures thereof; and (b) a polypeptide having lipase activity selected from the group consisting of: (I) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2; (II) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, or (ii) the full-length complement of (i); (III) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; (IV) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and (V) a fragment of the polypeptide of (I), (II), (III), or (IV); wherein said composition is a cleaning and/or treatment composition. The invention furthermore relates to methods of producing and using the composition.