公开(公告)号：US10472617B2

公开(公告)日：2019-11-12

申请号：US15714292

申请日：2017-09-25

Applicant: Novozymes A/S

Inventor： Tomoko Matsui ,  Aki Tomiki ,  Allan Svendsen ,  Hanne Vang Hendriksen ,  Mary Ann Stringer ,  Keiichi Ayabe

IPC: C12N9/82 ,  A23L5/20

Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.

公开(公告)号：US09879241B2

公开(公告)日：2018-01-30

申请号：US14426051

申请日：2013-09-06

Applicant: NOVOZYMES A/S

Inventor： Jesper Vind ,  Esben Peter Friis ,  Thomas Agsersten Poulsen ,  Michael Skjøt ,  Peter Kamp Hansen ,  Frank Winther Rasmussen ,  Steen Krogsgaard ,  Joyce Craig ,  Guillermo Coward-Kelly ,  Keiichi Ayabe

IPC: C12N9/24 ,  C12P7/04 ,  C12N9/34 ,  C12N9/30 ,  C12P7/10 ,  C12P19/02 ,  C12P19/24 ,  C12N15/52

CPC classification number: C12N9/2428 ,  C12N9/242 ,  C12N15/52 ,  C12P7/10 ,  C12P19/02 ,  C12P19/24 ,  Y02E50/16

Abstract: The present invention relates to variants of a parent glucoamylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the glucoamylase variants.

公开(公告)号：US20220090041A1

公开(公告)日：2022-03-24

申请号：US17547098

申请日：2021-12-09

Applicant: Novozymes A/S

Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki-Hashizume ,  Yuma Kurakata ,  Esben Peter Friis ,  Jens Erik Nielsen ,  Roland Alexander Pache

IPC: C12N9/58 ,  C12P7/06

Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10927361B2

公开(公告)日：2021-02-23

申请号：US16318496

申请日：2017-07-14

Applicant: NOVOZYMES A/S

Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben P. Friis ,  Jens E. Nielsen ,  Roland Alexander Pache

IPC: C12N9/58 ,  C12P7/06

Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20200172946A1

公开(公告)日：2020-06-04

申请号：US16788950

申请日：2020-02-12

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/16 ,  C12P19/02 ,  C12N9/44 ,  C13K1/06 ,  C12N9/34

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch, and to compositions useful therein.

公开(公告)号：US20170175095A1

公开(公告)日：2017-06-22

申请号：US15443931

申请日：2017-02-27

Applicant: Novozymes A/S

Inventor： Ming Li ,  Junxin Duan ,  Zheng Liu ,  Shiro Fukuyama ,  Keiichi Ayabe ,  Guillermo Coward-Kelly ,  Randall Deinhammer

IPC: C12N9/34 ,  C12N1/16

CPC classification number: C12N9/2428 ,  C12P7/06 ,  C12P7/08 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01003 ,  C13K1/06 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20150232824A1

公开(公告)日：2015-08-20

申请号：US14426051

申请日：2013-09-06

Applicant: NOVOZYMES A/S

Inventor： Jesper Vind ,  Esben Peter Friis ,  Thomas Agsersten Poulsen ,  Michael Skjøt ,  Peter Kamp Hansen ,  Frank Winther Rasmussen ,  Steen Krogsgaard ,  Joyce Craig ,  Guillermo Coward-Kelly ,  Keiichi Ayabe

IPC: C12N9/34 ,  C12N15/52 ,  C12P19/02 ,  C12P19/24 ,  C12N9/30 ,  C12P7/10

CPC classification number: C12N9/2428 ,  C12N9/242 ,  C12N15/52 ,  C12P7/10 ,  C12P19/02 ,  C12P19/24 ,  Y02E50/16

Abstract: The present invention relates to variants of a parent glucoamylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the glucoamylase variants.

Abstract translation: 本发明涉及母体葡糖淀粉酶的变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含所述多核苷酸的宿主细胞; 以及使用葡糖淀粉酶变体的方法。

公开(公告)号：US20220340843A1

公开(公告)日：2022-10-27

申请号：US17763592

申请日：2020-10-01

Applicant: Novozymes A/S

Inventor： Lone Baunsgaard ,  Michael Lynge Nielsen ,  Kenneth Jensen ,  Hiroshi Teramoto ,  Keiichi Ayabe

IPC: C11D3/386 ,  C12N9/42 ,  C11D11/00

Abstract: Disclosed are fusion polypeptides comprising at least two carbohydrate binding modules (CBMs), wherein the polypeptide has carbohydrate binding activity. Also disclosed is the use of such polypeptides for reducing the wrinkles in laundry, as well as detergent compositions and laundry booster compositions comprising the same. Also disclosed are polynucleotides encoding the variants, nucleic acid constructs, vectors, and host cells comprising the variants and methods of making the variants.

公开(公告)号：US11279921B2

公开(公告)日：2022-03-22

申请号：US16603639

申请日：2018-04-10

Applicant: NOVOZYMES A/S

Inventor： Tomoko Namoto ,  Noriko Tsutsumi ,  Keiichi Ayabe ,  Zhengfang Kang

IPC: C12N9/34 ,  C12P19/02 ,  C12P19/14

Abstract: The present invention relates to glucoamylase variants having an increase in raw starch activity compared to the guy-coamylase disclosed as SEQ ID NO: 2, comprising one or more modifications in the catalytic domain and/or one or more modifications in the starch binding domain selected from: a) at least one, preferably at least two, preferably at least three, preferably at least four of: V18M, T43K, N112L, T116R, A117Q, G120S, A271F, Y295W, Q318Y; and/or b) Introducing at least three, preferably at least four substitutions selected from the group: S458C, S458SCGG, S458SGGC, A493V, A518K, E520Q, N527M, S540K, R, S(G)546P, T(V)549W, N503R, N539R+I541Y+T543V+A545S+S546GCGV+G547S+S548T+T549A. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10538752B2

公开(公告)日：2020-01-21

申请号：US15679805

申请日：2017-08-17

Applicant: NOVOZYMES A/S

Inventor： Guillermo Coward-Kelly ,  Shiro Fukuyama ,  Noriko Tsutsumi ,  Keiichi Ayabe

IPC: C12N9/26 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/30 ,  C12G3/02 ,  C12P19/14 ,  C12P21/02 ,  C12P7/06 ,  C12C7/04 ,  C12C5/00

Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.