公开(公告)号：US09695429B2

公开(公告)日：2017-07-04

申请号：US14850448

申请日：2015-09-10

Applicant: Novozymes, Inc.

Inventor： Debbie Yaver ,  Peter Nham

IPC: C12N15/80 ,  C12P21/00 ,  C12P1/02 ,  C12N15/113

CPC classification number: C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

公开(公告)号：US20170137796A1

公开(公告)日：2017-05-18

申请号：US15394554

申请日：2016-12-29

Applicant: Novozymes Inc.

Inventor： Suzanne Otani ,  Haiyan Ge ,  Paul Harris ,  Debbie Yaver ,  Alexander Blinkovsky

IPC: C12N9/26 ,  C12C7/04 ,  C12C5/00

CPC classification number: C12N9/2408 ,  C12C5/004 ,  C12C7/04 ,  C12C7/20 ,  C12C11/00 ,  C12C12/02 ,  C12N9/2411 ,  C12N15/8245 ,  C12Y302/0102

Abstract: The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

公开(公告)号：US09539311B2

公开(公告)日：2017-01-10

申请号：US14679563

申请日：2015-04-06

Applicant: Novozymes A/S ,  Novozymes Inc.

Inventor： Allan Svendsen ,  Michael Skjoet ,  Debbie Yaver ,  Lars Lehmann Hylling Christensen ,  Signe Eskildsen Larsen ,  Nina Lundin ,  Michael Lamsa ,  Peter Colin Gregory

IPC: A61K38/47 ,  A61K38/48 ,  A61K38/54 ,  A61K38/46 ,  C12N9/20 ,  C12N9/14 ,  C12N9/26 ,  C12N9/48 ,  C07H21/04 ,  C07K1/00

CPC classification number: A61K38/465 ,  A61K38/47 ,  A61K38/48 ,  C12N9/20

Abstract: The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, an are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.

Abstract translation: 与包含SEQ ID NO：2的氨基酸1-269的Thermomyces lanuginosus（Humicola lanuginosa）脂肪酶相关的脂肪酶的药物用途，任选地与蛋白酶和/或淀粉酶组合。 医学适应症的例子有：消化系统疾病，胰腺外分泌功能不全（PEI），胰腺炎，囊性纤维化，I型糖尿病和/或II型糖尿病的治疗。 本发明的脂肪酶具有例如体外改进的消化性能，在中性范围内的pH值改善的活性，在低pH下的改进的稳定性，对蛋白酶降解是稳定的，和/或在存在时是稳定的 的胃蛋白酶和胆汁盐。 本发明还涉及确定脂肪酶的体外消化性能的方法以及T.lanuginosus的脂肪酶的某些新颖变体。

公开(公告)号：US20160251666A1

公开(公告)日：2016-09-01

申请号：US15156857

申请日：2016-05-17

Applicant: Novozymes, Inc.

Inventor： Michael Catlett ,  Debbie Yaver

IPC: C12N15/81 ,  C12P21/00

CPC classification number: C12N15/815 ,  C12N15/80 ,  C12P21/00 ,  C12P21/06

Abstract: The present invention relates to isolated promoters and constructs, vectors, and fungal host cells comprising such promoters operably linked to polynucleotides encoding polypeptides. The present invention also relates to methods for producing such polypeptides.

公开(公告)号：US20160168547A1

公开(公告)日：2016-06-16

申请号：US15051169

申请日：2016-02-23

Applicant: Novozymes Inc. ,  Novozymes A/S

Inventor： Suzanne Otani ,  Debbie Yaver ,  Haiyan Ge ,  Janine Lin ,  Christopher Amolo ,  Kim Borch ,  Shamkant Anant Patkar ,  Michael Lamsa ,  Barbara Cherry

IPC: C12N9/20

CPC classification number: C12N9/20 ,  C12Y301/01003

Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Abstract translation: 本发明涉及具有脂肪酶活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。

公开(公告)号：US20160152993A1

公开(公告)日：2016-06-02

申请号：US15014764

申请日：2016-02-03

Applicant: Novozymes, Inc.

Inventor： Sandra Merino ,  Debbie Yaver

IPC: C12N15/80

CPC classification number: C12N15/80 ,  C12N9/0065 ,  C12P21/00

Abstract: The present invention relates to isolated promoters and constructs, vectors, and fungal host cells comprising such promoters operably linked to polynucleotides encoding polypeptides. The present invention also relates to methods for producing such polypeptides.

公开(公告)号：US09303275B2

公开(公告)日：2016-04-05

申请号：US13948945

申请日：2013-07-23

Applicant: Novozymes, Inc.

Inventor： Amanda Fischer ,  Debbie Yaver

IPC: C12P7/46 ,  C12N15/31 ,  C07K14/38 ,  C12N9/04 ,  C12N9/88 ,  C12N15/80

CPC classification number: C12P7/46 ,  C07K14/38 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/80 ,  C12Y604/01001

Abstract: The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.

公开(公告)号：US09273104B2

公开(公告)日：2016-03-01

申请号：US14081340

申请日：2013-11-15

Applicant: Novozymes A/S ,  Novozymes, Inc.

Inventor： Mads Bjornvad ,  Debbie Yaver

IPC: C07K7/00 ,  C12N9/20 ,  C07K14/37 ,  C12N15/62 ,  C12N15/81 ,  C12P21/02 ,  A61K38/00

CPC classification number: C12P21/02 ,  C07K14/37 ,  C07K2319/02 ,  C12N9/20 ,  C12N15/625 ,  C12N15/81 ,  C12Y301/01003

Abstract: The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium.

公开(公告)号：US20150376630A1

公开(公告)日：2015-12-31

申请号：US14850448

申请日：2015-09-10

Applicant: Novozymes, Inc.

Inventor： Debbie Yaver ,  Peter Nham

IPC: C12N15/80 ,  C12P21/00

CPC classification number: C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

Abstract translation: 本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列 连接到第二核酸序列，其包含选自SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO： ：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11和SEQ ID NO：12; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。 本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。

公开(公告)号：US09150856B2

公开(公告)日：2015-10-06

申请号：US14097659

申请日：2013-12-05

Applicant: Novozymes, Inc.

Inventor： Debbie Yaver ,  Peter Nham

IPC: C12P21/00 ,  C12N15/113 ,  C12N15/80 ,  C12P1/02

CPC classification number: C12N15/80 ,  C12N9/0044 ,  C12N9/20 ,  C12N9/58 ,  C12N9/93 ,  C12N15/113 ,  C12P1/02 ,  C12P21/00 ,  C12Y107/01003 ,  C12Y301/01003 ,  C12Y304/21004 ,  C12Y603/04002

Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

Abstract translation: 本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列 连接到第二核酸序列，其包含选自SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO： ：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11和SEQ ID NO：12; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。 本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。