-
公开(公告)号：US11981917B2
公开(公告)日：2024-05-14
申请号：US17972885
申请日：2022-10-25
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Prashant G. Mali , Kevin M. Esvelt
CPC classification number: C12N15/907 , C12N9/22 , C12N15/102 , C12N15/11 , C12N15/113 , C12N15/635 , C12N2310/20 , C12N2310/3513 , C12Y301/00
Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.
-
公开(公告)号：US20230257781A1
公开(公告)日：2023-08-17
申请号：US18296446
申请日：2023-04-06
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Kevin M. Esvelt , Prashant G. Mali
CPC classification number: C12N15/907 , C12N15/85 , C12N9/22 , C12N15/113 , C07K2/00 , C12N15/11 , C12N2800/40 , C12N2800/80 , C12N2810/10 , C12N2830/00 , C12N2830/34 , C12N2999/007 , C12N2320/33 , C12N2320/50 , C12N2310/3519 , C12N2800/107 , C12Y301/00
Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.
-
公开(公告)号：US20200048656A1
公开(公告)日：2020-02-13
申请号：US16439840
申请日：2019-06-13
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Prashant G. Mali , Luhan Yang
IPC: C12N15/85 , C12N9/22 , C12N15/10 , C12N15/90 , C12N15/87 , C12N15/82 , C12N15/81 , C12N15/01 , C12N15/63
Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
-
公开(公告)号：US10273501B2
公开(公告)日：2019-04-30
申请号：US14790147
申请日：2015-07-02
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Prashant G. Mali , Luhan Yang
IPC: C12N9/22 , C12N15/85 , C12N15/10 , C12N15/63 , C12N15/90 , C12N15/01 , C12N15/81 , C12N15/82 , C12N15/87
Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
-
公开(公告)号：US20160304907A1
公开(公告)日：2016-10-20
申请号：US15042573
申请日：2016-02-12
Applicant: President and Fellows of Harvard College
Inventor： Prashant G. Mali , George M. Church , Luhan Yang
IPC: C12N15/90
Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
-
Patent Agency Ranking
公开(公告)号：US20160237456A1
公开(公告)日：2016-08-18
申请号：US15049451
申请日：2016-02-22
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Prashant G. Mali , Kevin M. Esvelt
CPC classification number: C12N15/907 , C12N9/22 , C12N15/102 , C12N15/11 , C12N15/113 , C12N15/635 , C12N2310/20 , C12N2310/3513 , C12Y301/00
Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.
Abstract translation: 提供了调节细胞中靶核酸表达的方法，包括向细胞中引入编码与DNA互补的一个或多个RNA的第一外源核酸，其中DNA包括靶核酸，将第二外源核酸 编码与所述DNA结合并由所述一个或多个RNA引导的核酸酶 - 无效Cas9蛋白的酸，其将所述一个或多个RNA引入所述细胞中，所述第三外源核酸编码转录调节蛋白或结构域，其中所述核酸酶 - 表达无效Cas9蛋白和转录调节蛋白或结构域，其中一个或多个RNA，核酸酶无效Cas9蛋白和转录调节蛋白或结构域共定位于DNA，并且其中转录调节蛋白或结构域调节表达 的靶核酸。
-
公开(公告)号：US20160222416A1
公开(公告)日：2016-08-04
申请号：US14903728
申请日：2014-07-08
Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Inventor： George M. Church , Kevin M. Esvelt , Prashant G. Mali
CPC classification number: C12N15/907 , C07K2/00 , C12N9/22 , C12N15/11 , C12N15/113 , C12N15/85 , C12N2310/3519 , C12N2320/33 , C12N2320/50 , C12N2800/107 , C12N2800/40 , C12N2800/80 , C12N2810/10 , C12N2830/00 , C12N2830/34 , C12N2999/007 , C12Y301/00
Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.
-
公开(公告)号：US20160160210A1
公开(公告)日：2016-06-09
申请号：US15042515
申请日：2016-02-12
Applicant: President and Fellows of Harvard College
Inventor： Prashant G. Mali , George M. Church , Luhan Yang
Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner
Abstract translation: 提供了改变真核细胞的方法，包括用编码与真核细胞的基因组DNA互补的RNA的核酸转染真核细胞，用编码与RNA相互作用并切割基因组DNA的酶的核酸转染真核细胞 以位点特异性方式，其中细胞表达RNA和酶，RNA结合互补基因组DNA，酶以位点特异性方式切割基因组DNA
-
公开(公告)号：US12018272B2
公开(公告)日：2024-06-25
申请号：US18296579
申请日：2023-04-06
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Prashant G. Mali , Luhan Yang
IPC: C12N15/63 , C12N9/22 , C12N15/01 , C12N15/10 , C12N15/81 , C12N15/82 , C12N15/85 , C12N15/87 , C12N15/90 , A61K48/00
CPC classification number: C12N15/85 , C12N9/22 , C12N15/01 , C12N15/10 , C12N15/102 , C12N15/1024 , C12N15/63 , C12N15/81 , C12N15/8201 , C12N15/87 , C12N15/90 , C12N15/907 , C12N2310/20 , C12N2800/80 , C12N2810/55 , C12Y301/00
Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
-
公开(公告)号：US11365429B2
公开(公告)日：2022-06-21
申请号：US16397213
申请日：2019-04-29
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Prashant G. Mali , Luhan Yang
IPC: C12N15/00 , C12N15/85 , C12N15/10 , C12N15/63 , C12N15/90 , C12N15/01 , C12N15/81 , C12N15/82 , C12N15/87 , C12N9/22
Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
-
-
-
-
-
-
-
-
-
Search Results
39
records
(took 0.03 seconds)
