公开(公告)号：US11732258B2

公开(公告)日：2023-08-22

申请号：US16346569

申请日：2017-11-01

Applicant: President and Fellows of Harvard College

Inventor： Reza Kalhor ,  Prashant G. Mali ,  George M. Church

IPC: C12N15/00 ,  C12N15/11 ,  C12N15/10 ,  C12Q1/6853 ,  C12N9/22 ,  C12N15/113

CPC classification number: C12N15/11 ,  C12N9/22 ,  C12N15/10 ,  C12N15/1096 ,  C12N15/113 ,  C12Q1/6853 ,  C12N2310/20 ,  C12N2310/3519 ,  C12N2310/531 ,  C12Q1/6853 ,  C12Q2521/107 ,  C12Q2525/155 ,  C12Q2525/197 ,  C12Q2525/301

Abstract: A functional engineered guide RNA sequence is provided including a spacer sequence and a scaffold sequence, wherein the scaffold sequence includes a primer binding site for reverse transcription.

公开(公告)号：US11649469B2

公开(公告)日：2023-05-16

申请号：US16411793

申请日：2019-05-14

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Kevin M. Esvelt ,  Prashant G. Mali

IPC: C12N15/00 ,  C12N15/90 ,  C12N15/85 ,  C12N9/22 ,  C12N15/113 ,  C07K2/00 ,  C12N15/11

CPC classification number: C12N15/907 ,  C07K2/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N15/85 ,  C12N2310/3519 ,  C12N2320/33 ,  C12N2320/50 ,  C12N2800/107 ,  C12N2800/40 ,  C12N2800/80 ,  C12N2810/10 ,  C12N2830/00 ,  C12N2830/34 ,  C12N2999/007 ,  C12Y301/00

Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.

公开(公告)号：US11512325B2

公开(公告)日：2022-11-29

申请号：US17672744

申请日：2022-02-16

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/00 ,  C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20200308599A1

公开(公告)日：2020-10-01

申请号：US16884327

申请日：2020-05-27

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US10767194B2

公开(公告)日：2020-09-08

申请号：US16441209

申请日：2019-06-14

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Kevin M. Esvelt

IPC: C12N15/113 ,  C12N15/90 ,  C12N15/10 ,  C12N15/63 ,  C12N9/22 ,  C12N15/11

Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

公开(公告)号：US20190161743A1

公开(公告)日：2019-05-30

申请号：US16099473

申请日：2017-05-09

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Reza Kalhor ,  Prashant G. Mali

IPC: C12N9/22 ,  C12N15/11

Abstract: CRISPR/Cas9 methods are provided where a guide RNA is engineered to self-target and inactivate a nucleic acid encoding the guide RNA itself.

公开(公告)号：US20240175057A1

公开(公告)日：2024-05-30

申请号：US18425219

申请日：2024-01-29

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Kevin M. Esvelt

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/635 ,  C12N2310/20 ,  C12N2310/3513 ,  C12Y301/00

Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

公开(公告)号：US20230295653A1

公开(公告)日：2023-09-21

申请号：US18296579

申请日：2023-04-06

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

CPC classification number: C12N15/85 ,  C12N15/10 ,  C12N15/102 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/907 ,  C12N15/1024 ,  C12N9/22 ,  C12N2310/20 ,  C12N2810/55 ,  C12Y301/00 ,  C12N2800/80

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20230272380A1

公开(公告)日：2023-08-31

申请号：US18313482

申请日：2023-05-08

Applicant: President and Fellows of Harvard College

Inventor： Reza Kalhor ,  Prashant G. Mali ,  George M. Church

IPC: C12N15/11 ,  C12N15/10 ,  C12Q1/6853 ,  C12N9/22 ,  C12N15/113

CPC classification number: C12N15/11 ,  C12N15/1096 ,  C12Q1/6853 ,  C12N15/10 ,  C12N9/22 ,  C12N15/113 ,  C12N2310/20 ,  C12N2310/3519 ,  C12N2310/531

Abstract: A functional engineered guide RNA sequence is provided including a spacer sequence and a scaffold sequence, wherein the scaffold sequence includes a primer binding site for reverse transcription.

公开(公告)号：US20230131972A1

公开(公告)日：2023-04-27

申请号：US17972885

申请日：2022-10-25

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Kevin M. Esvelt

IPC: C12N15/90 ,  C12N15/113 ,  C12N15/10 ,  C12N15/63 ,  C12N9/22 ,  C12N15/11

Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.