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公开(公告)号：US20150336072A1
公开(公告)日：2015-11-26
申请号：US14812964
申请日：2015-07-29
发明人： David A. Weitz , Jeremy Agresti
IPC分类号： B01J19/00
CPC分类号： C12Q1/6876 , B01J13/04 , B01J19/0046 , B01J2219/00599 , B01J2219/00709 , B01J2219/00711 , B01J2219/00716 , B01J2219/00722 , C12Q1/6874
摘要： The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.
摘要翻译： 本发明通常涉及用于生产多个液滴的系统和方法。 液滴可以包含不同的物种，例如用作文库。 在一些情况下，流体液滴可以被硬化以形成刚性液滴（例如凝胶液滴）。 在某些实施方案中，液滴可能经历相变（例如，从刚性液滴到流化液滴），如本文更多地讨论的。 在一些情况下，通过将液滴暴露于包含多种物质的流体，物质可以内部添加到液滴中。
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公开(公告)号：US20150336068A1
公开(公告)日：2015-11-26
申请号：US14812930
申请日：2015-07-29
发明人： David A. Weitz , Jeremy Agresti
CPC分类号： C12Q1/6876 , B01J13/04 , B01J19/0046 , B01J2219/00599 , B01J2219/00709 , B01J2219/00711 , B01J2219/00716 , B01J2219/00722 , C12Q1/6874
摘要： The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.
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公开(公告)号：US09068210B2
公开(公告)日：2015-06-30
申请号：US14172326
申请日：2014-02-04
发明人： Jeremy Agresti , Liang-Yin Chu , David A. Weitz , Jin-Woong Kim , Amy Rowat , Morten Sommer , Gautam Dantas , George Church
CPC分类号： C12P19/34 , B01F3/0807 , B01F3/0811 , B01F13/0062 , B01F13/0071 , B01F2215/0037 , B01J13/0052 , B01J13/0065 , B01L3/502784 , C12Q1/6834 , C12Q1/6848 , C12Q1/686 , G01N15/1404 , G01N2015/1006
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
摘要翻译： 本发明通常涉及液滴和/或乳液，例如多重乳液。 在一些情况下，液滴和/或乳液可用于测定中，并且在某些实施方案中，液滴或乳液可被硬化以形成凝胶。 在一些方面，可以使用凝胶进行异质测定。 例如，液滴可以被硬化以形成凝胶，其中液滴包含细胞，DNA或其它合适的物质。 凝胶可以暴露于反应物，并且反应物可以以某种方式与凝胶和/或与细胞，DNA等相互作用。 例如，反应物可以扩散通过凝胶，或者硬化的颗粒可以液化以形成液体状态，允许反应物与细胞相互作用。 作为具体实例，包含在凝胶颗粒内的DNA可以进行PCR（聚合酶链式反应）扩增，例如通过使用能够在形成凝胶时结合凝胶的PCR引物。 当使用PCR扩增DNA时，一些DNA将通过PCR引物与凝胶结合。 PCR反应后，未结合的DNA可以从凝胶中去除，例如通过扩散或洗涤。 因此，可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。
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公开(公告)号：US11498072B2
公开(公告)日：2022-11-15
申请号：US17060354
申请日：2020-10-01
发明人： David A. Weitz , Christian Boehm , Amy Rowat , Sarah Koester , Jeremy Agresti
摘要： The present invention relates to systems and methods for the arrangement of droplets in pre-determined locations. Many applications require the collection of time-resolved data. Examples include the screening of cells based on their growth characteristics or the observation of enzymatic reactions. The present invention provides a tool and related techniques which addresses this need, and which can be used in many other situations. The invention provides, in one aspect, a tool that allows for stable storage and indexing of individual droplets. The invention can interface not only with microfluidic/microscale equipment, but with macroscopic equipment to allow for the easy injection of liquids and extraction of sample droplets, etc.
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公开(公告)号：US20220212194A1
公开(公告)日：2022-07-07
申请号：US17550612
申请日：2021-12-14
发明人： David A. Weitz , Thomas Franke , Achim Wixforth , Lothar Schmid , Jeremy Agresti , Adam R. Abate
摘要： Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated front the microfluidic substrate except at or proximate the location where the droplets arc sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.
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公开(公告)号：US10738337B2
公开(公告)日：2020-08-11
申请号：US16431354
申请日：2019-06-04
发明人： David A. Weitz , Jeremy Agresti , Liang-Yin Chu , Jin-Woong Kim , Amy Rowat , Morten Sommer , Gautam Dantas , George M. Church
IPC分类号： C12P19/34 , C12Q1/68 , B01J13/00 , B01F13/00 , B01F3/08 , G01N15/14 , C12Q1/686 , C12Q1/6848 , C12Q1/6834 , B01L3/00 , G01N15/10
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
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公开(公告)号：US10570361B2
公开(公告)日：2020-02-25
申请号：US15604085
申请日：2017-05-24
发明人： David A. Weitz , Thomas Franke , Achim Wixforth , Lothar Schmid , Jeremy Agresti , Adam R. Abate
摘要： Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.
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公开(公告)号：US10508294B2
公开(公告)日：2019-12-17
申请号：US15792218
申请日：2017-10-24
发明人： David A. Weitz , Jeremy Agresti , Liang-Yin Chu , Jin-Woong Kim , Amy Rowat , Morten Sommer , Gautam Dantas , George Church
IPC分类号： C12P19/34 , B01J13/00 , B01F13/00 , B01F3/08 , C12Q1/686 , C12Q1/6848 , C12Q1/6834 , G01N15/14 , B01L3/00 , G01N15/10
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
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公开(公告)号：US20150336071A1
公开(公告)日：2015-11-26
申请号：US14812951
申请日：2015-07-29
发明人： David A. Weitz , Jeremy Agresti
CPC分类号： C12Q1/6876 , B01J13/04 , B01J19/0046 , B01J2219/00599 , B01J2219/00709 , B01J2219/00711 , B01J2219/00716 , B01J2219/00722 , C12Q1/6874
摘要： The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.
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公开(公告)号：US20150336069A1
公开(公告)日：2015-11-26
申请号：US14812942
申请日：2015-07-29
发明人： David A. Weitz , Jeremy Agresti
IPC分类号： B01J19/00
摘要： The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.
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