利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

129 条记录 (耗时 0.069 秒)

    Monooxygenase assays
    21.
    发明授权
    Monooxygenase assays 失效
    单加氧酶测定

    公开(公告)号：US06632630B2

    公开(公告)日：2003-10-14

    申请号：US09820289

    申请日：2001-03-28

    申请人： Sharat Singh ,  Ahmed Chenna ,  Hossein Salimi-Moosari ,  Ian Gibbons

    发明人： Sharat Singh ,  Ahmed Chenna ,  Hossein Salimi-Moosari ,  Ian Gibbons

    IPC分类号： C12Q126

    CPC分类号： C12Q1/26 ,  G01N2333/795 ,  G01N2333/80 ,  G01N2333/90245 ,  Y10S435/968

    摘要： Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.

    摘要翻译： 使用细胞色素P-450酶提供细胞色素P-450测定方法和用于该方法的试剂盒，其特征在于具有氧化成醛的可氧化亚甲基和荧光肼。 向反应混合物中加入荧光肼，通过毛细管电泳分析得到的腙。 该方法可用于评价化合物的酶调节活性

    Methods for single nucleotide polymorphism detection
    22.
    发明授权
    Methods for single nucleotide polymorphism detection 失效
    单核苷酸多态性检测方法

    公开(公告)号：US06632606B1

    公开(公告)日：2003-10-14

    申请号：US09592053

    申请日：2000-06-12

    申请人： Edwin F. Ullman ,  Sharat Singh

    发明人： Edwin F. Ullman ,  Sharat Singh

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6827 ,  C12Q1/6834 ,  C12Q2565/519 ,  C12Q2535/125 ,  C12Q2563/179 ,  C12Q2563/131

    摘要： Methods and compositions are provided for determining large numbers of single nucleotide polymorphisms in target DNA employing particles having (1) primers complementary to sequences in the target DNA where the next succeeding 3′-nucleotide is a potential single nucleotide polymorphism and coding composition members, where the members are unique for each primer, and (2) differentially labeled terminating nucleotides, where the label permits separation of the terminating nucleotides. Desirably the particles are separated into groups having a common prevalent next succeeding nucleotide. The particles and target DNA are combined under nucleotide extending conditions, the particles separated into groups in accordance with the terminating nucleotide and the coding members identified, so that one knows the sequence and the single nucleotide polymorphism. Various protocols are provided for the determination.

    摘要翻译： 提供了用于使用具有（1）与目标DNA中的序列互补的引物，其中下一个后续的3'-核苷酸是潜在的单核苷酸多态性和编码组成成员的粒子来确定靶DNA中大量单核苷酸多态性的方法和组合物，其中 成员对于每个引物是独特的，和（2）差异标记的终止核苷酸，其中标记允许分离终止的核苷酸。 理想地，将颗粒分离成具有普通的下一个后续核苷酸的基团。 颗粒和靶DNA在核苷酸延伸条件下合并，根据终止的核苷酸和鉴定的编码成员将颗粒分成组，以便知道序列和单核苷酸多态性。 提供各种协议用于确定。

    Metal chelate containing compositions for use in chemiluminescent assays
    23.
    发明授权
    Metal chelate containing compositions for use in chemiluminescent assays 失效
    含金属螯合物的组合物用于化学发光测定

    公开(公告)号：US06180354B2

    公开(公告)日：2001-01-30

    申请号：US08480430

    申请日：1995-06-06

    申请人： Sharat Singh ,  Edwin F. Ullman

    发明人： Sharat Singh ,  Edwin F. Ullman

    IPC分类号： G01N3353

    CPC分类号： C07D265/30 ,  C07D279/12 ,  C07D327/06 ,  C07D413/04 ,  G01N33/533 ,  G01N33/54313 ,  G01N33/5436 ,  G01N33/58 ,  G01N33/582 ,  G01N33/585 ,  G01N33/586 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/808 ,  Y10T436/13

    摘要： Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.

    摘要翻译： 公开了包含（a）金属螯合物的组合物，其中金属选自铕，铽，镝，钐锇和钌至少六配位状态，和（b）具有被两个芳基取代的双键的化合物 基团，氧原子和选自氧，硫和氮的原子，其中一个芳基相对于另一个赋予电子。 这种组合物优选掺入胶乳颗粒材料中。 还公开了用于测定怀疑含有分析物的培养基中的分析物的方法和试剂盒。 所述方法和试剂盒采用如上所述的组合物作为一种组分。

    Chemiluminescent compounds and methods of use
    24.
    发明授权
    Chemiluminescent compounds and methods of use 失效
    化学发光化合物和使用方法

    公开(公告)号：US5936070A

    公开(公告)日：1999-08-10

    申请号：US664269

    申请日：1996-06-11

    申请人： Sharat Singh ,  Rajendra Singh ,  Frank Meneghini ,  Edwin F. Ullman

    发明人： Sharat Singh ,  Rajendra Singh ,  Frank Meneghini ,  Edwin F. Ullman

    IPC分类号： G01N33/532 ,  C07D471/10 ,  C07D471/20 ,  C07D491/10 ,  C07D491/113 ,  C07D498/10 ,  C07D517/10 ,  G01N21/78 ,  G01N33/533 ,  G01N33/58 ,  G01N33/531 ,  G01N33/576 ,  G01N33/76

    CPC分类号： C07D471/10 ,  C07D491/10 ,  G01N33/533 ,  G01N33/582 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/815 ,  Y10S435/818

    摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises providing (1) combining a medium suspected of containing the analyte and a novel chemiluminescent compound, (2) combining a means for chemically activating the chemiluminescent compound; and (3) detecting the amount of luminescence generated by the chemiluminescent compound. The amount of luminescence generated is related to the amount of analyte in the medium. The chemiluminescent compound can be chemically activated by hydrogen peroxide. Compositions and kits are also disclosed.The chemiluminiscent compound is a spiro-acridan and has ##STR1## where X and Y are independently O, S, Se or NH; and Z is a chain 1-5 atoms in length; 0 to 8 hydrogens of the compound alone or taken together, may be replaced an alkyl, alkylidine, aryl aralkyl, or an alkyl, aryl or aralkyl substituted with one or more radicals of functional groups; 1 to 4 of the aromatic carbon atoms may be replaced by nitrogen atoms; and 0 to 1 hydrogens may be replaced by a specific binding pair member or fluorescent group.

    摘要翻译： 公开了用于确定怀疑含有分析物的介质中的分析物的方法。 一种方法包括提供（1）将怀疑含有分析物的介质与新型化学发光化合物组合，（2）组合化学发光化合物的化学活化装置; 和（3）检测由化学发光化合物产生的发光量。 产生的发光量与介质中分析物的量有关。 化学发光化合物可以被过氧化氢化学活化。 还公开了组合物和试剂盒。 化学发光化合物是螺 - 吖啶，并且其中X和Y独立地为O，S，Se或NH; Z是长度为1-5个原子的链; 可以单独或一起取代的化合物的0至8个氢可以被烷基，烷基锡，芳基芳烷基或被一个或多个官能团基团取代的烷基，芳基或芳烷基取代; 1至4个芳族碳原子可被氮原子取代; 并且0至1个氢可以被特异性结合对成员或荧光基团代替。

    Citrullinated peptides for diagnosing and prognosing rheumatoid arthritis
    28.
    发明授权
    Citrullinated peptides for diagnosing and prognosing rheumatoid arthritis 有权
    用于诊断和预测类风湿性关节炎的瓜氨酸肽

    公开(公告)号：US08519096B2

    公开(公告)日：2013-08-27

    申请号：US13239329

    申请日：2011-09-21

    申请人： Nicholas Chi-Kwan Ling ,  Shui Long Wang ,  Dunrui Wang ,  Sharat Singh

    发明人： Nicholas Chi-Kwan Ling ,  Shui Long Wang ,  Dunrui Wang ,  Sharat Singh

    IPC分类号： G01N33/531 ,  G01N33/533 ,  C07K17/00

    CPC分类号： G01N33/564 ,  C07K14/4713

    摘要： The present invention provides novel citrullinated peptides, their use in methods for aiding, assisting, improving, or facilitating the diagnosis or prognosis of rheumatic diseases such as rheumatoid arthritis (RA), and methods for identifying novel citrullinated peptides that are immunoreactive with anti-citrullinated protein antibodies (ACPAs). The present invention also provides methods for detecting rheumatoid factor (RF) using novel RF detection reagents as a means to aid, assist, improve, or facilitate the diagnosis or prognosis of rheumatic diseases such as RA. Kits comprising at least one of the novel citrullinated peptides and/or RF detection reagents of the present invention are also provided.

    摘要翻译： 本发明提供新颖的瓜氨酸肽，其用于帮助，协助，改善或促进风湿性疾病如类风湿性关节炎（RA）的诊断或预后的方法，以及用于鉴定与抗瓜氨酸化免疫反应的新型瓜氨酸肽 蛋白质抗体（ACPA）。 本发明还提供使用新的RF检测试剂检测类风湿因子（RF）的方法，以帮助，协助，改善或促进风湿性疾病如RA的诊断或预后。 还提供了包含本发明的新颖瓜氨酸肽和/或RF检测试剂中的至少一种的试剂盒。

    NOVEL GENOMIC BIOMARKERS FOR IRRITABLE BOWEL SYNDROME DIAGNOSIS
    30.
    发明申请
    NOVEL GENOMIC BIOMARKERS FOR IRRITABLE BOWEL SYNDROME DIAGNOSIS 审中-公开
    新型基因组生物标志物用于有用的BOWEL综合征诊断

    公开(公告)号：US20130005596A1

    公开(公告)日：2013-01-03

    申请号：US13479127

    申请日：2012-05-23

    申请人： Hua Gong ,  Sharat Singh ,  Nicholas Hoe

    发明人： Hua Gong ,  Sharat Singh ,  Nicholas Hoe

    IPC分类号： C40B30/04 ,  G01N27/62 ,  C12Q1/68

    CPC分类号： C12Q1/6883 ,  C12Q2600/112 ,  C12Q2600/158 ,  G01N33/6893 ,  G01N2800/065

    摘要： The invention provides novel biomarkers, kits, and methods of diagnosing, prognosing, and subtyping IBS. In one aspect, the invention provides novel genomic biomarkers for diagnosing, classifying, providing a prognosis for, and assigning therapy for IBS in a subject in need thereof. In another aspect, the present invention provides novel algorithms for the diagnosis and prognosis of IBS.

    摘要翻译： 本发明提供了诊断，预后和亚型IBS的新型生物标志物，试剂盒和方法。 一方面，本发明提供用于诊断，分类，提供有需要的受试者的IBS预后和分配治疗的新型基因组生物标志物。 另一方面，本发明提供了用于IBS诊断和预后的新颖算法。