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    Method for maximization of artemisinin production by the plant artemisia annua
    21.
    发明授权
    Method for maximization of artemisinin production by the plant artemisia annua 失效
    青蒿植物青蒿素生产最大化的方法

    公开(公告)号：US06393763B1

    公开(公告)日：2002-05-28

    申请号：US09538892

    申请日：2000-03-30

    申请人： Sushil Kumar ,  Shiv Kumar Gupta ,  Madan Mohan Gupta ,  Ram Kishor Verma ,  Dharam Chand Jain ,  Ajit Kumar Shasany ,  Mahendra Pandurang Darokar ,  Suman Preet Singh Khanuja

    发明人： Sushil Kumar ,  Shiv Kumar Gupta ,  Madan Mohan Gupta ,  Ram Kishor Verma ,  Dharam Chand Jain ,  Ajit Kumar Shasany ,  Mahendra Pandurang Darokar ,  Suman Preet Singh Khanuja

    IPC分类号： A01B7900

    CPC分类号： A01H3/00

    摘要： The invention provides a method for maximization of artemisinin yield of the plant Artemisia annua, said method comprising sowing seeds of Artemisia annua plant on raised bed nursery during second and third week of December and maintaining the moisture throughout; transplanting seedlings thus obtained bearing at least 5-15 leaves into the main field fertilized with fertilizer, preferably NPK @ 80,40,40 kg/ha to achieve a population density of 50,000 to 200,000 per ha followed by light irrigation in the second week of March and irrigation every fortnight thereafter; harvesting the crop four times by cutting the plant tops leaving 75-100 cm part of plant for further regeneration, the said harvests are performed in a manner that the first harvest is done in fourth week of May, second harvest in third week of July, third harvest in second week of September and fourth harvest in third week of October of each year; and at each harvesting time care is taken to care at least one green branch, and extracting artemisinin from the plant tissue immediately after each harvest.

    摘要翻译： 本发明提供了一种使青蒿植物青蒿素产量最大化的方法，所述方法包括在12月的第二和第三周在秧苗上播种蒿种子，并保持水分; 将由此获得的至少5-15叶的幼苗移植到用肥料施肥的主田中，优选为80,40,40kg / ha的NPK，以实现每公顷50,000至20万的人口密度，然后在第二周进行轻度灌溉 三月份之后每两周灌溉一次; 通过切割植物顶部将作物收割四次，留下75-100cm部分的植物进行进一步再生，所述收获的进行方式是在5月的第4周进行第一次收获，在7月的第3周进行第二次收获， 9月第二周第三次收获，每年10月第三周收获第四季; 并且在每个收获时间处理至少一个绿色分支，并且在每次收获后立即从植物组织中提取青蒿素。