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公开(公告)号：US08144396B2
公开(公告)日：2012-03-27
申请号：US12060994
申请日：2008-04-02
IPC分类号： G02B21/26
CPC分类号： G02B21/33
摘要： An image can be prevented from becoming unclear over time during long-term observation. The invention provides a microscope apparatus including a specimen container for containing a specimen; an objective lens disposed opposite the specimen container for collecting light from the specimen in the specimen container; an immersion-liquid supplying unit for supplying immersion liquid to a space between the objective lens and the specimen container; and an immersion-liquid removing unit for removing the immersion liquid from the space between the objective lens and the specimen container. The immersion-liquid removing unit includes a nozzle for ejecting compressed air to the space between the objective lens and the specimen container.
摘要翻译： 在长期观察期间，可以防止图像变得不清楚。 本发明提供了一种显微镜装置，其包括：容纳样本的检体容器; 与样本容器相对设置的用于收集来自样本容器中的样本的光的物镜; 浸没液供给单元，其向所述物镜和所述检体容器之间的空间供给浸渍液; 以及用于从物镜和样本容器之间的空间中去除浸液的浸液清除单元。 浸液清除单元包括用于将压缩空气喷射到物镜和样本容器之间的空间的喷嘴。
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公开(公告)号：US07983466B2
公开(公告)日：2011-07-19
申请号：US11800119
申请日：2007-05-04
申请人： Mitsuhiro Nakano , Tatsuo Nakata
发明人： Mitsuhiro Nakano , Tatsuo Nakata
IPC分类号： G06K9/00
CPC分类号： C12M41/36 , G02B21/0012 , G02B21/16 , G02B21/32
摘要： The invention provides a microscope apparatus, used for identifying the cell phases of a plurality of cells mounted on a stage, including an analysis/observation optical system used for acquiring an observed-image of the cells and a stimulus optical system used for applying an optical stimulus to prescribed cells. Using these optical systems, the cell phases of the cells mounted on the stage are identified, an optical stimulus is applied to the cells, and the state of the cells before and after applying the stimulus is observed.
摘要翻译： 本发明提供了一种用于识别安装在舞台上的多个电池的电池相位的显微镜装置，包括用于获取电池的观察图像的分析/观察光学系统和用于施加光学器件的刺激光学系统 刺激规定的细胞。 使用这些光学系统，鉴定安装在载物台上的细胞的细胞相，向细胞施加光学刺激，并观察施加刺激之前和之后的细胞状态。
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公开(公告)号：US20110121198A1
公开(公告)日：2011-05-26
申请号：US12948937
申请日：2010-11-18
申请人： Tatsuo Nakata , Kosuke TAKAGI
发明人： Tatsuo Nakata , Kosuke TAKAGI
IPC分类号： G01J1/58
CPC分类号： G02B21/004 , G02B21/0076 , G02B21/008
摘要： Provided is a laser scanning microscope with which it is possible to reduce the time required for scanning and to detect light from a sample with high sensitivity. Provided is a laser scanning microscope 100 including a stage 20 that moves a sample 19 placed thereon, a laser light source 1 that emits laser light L, a line-focus optical system 3 that focuses the laser light L into a line shape, a DMD 7 including a plurality of movable micromirrors that are arrayed in the lengthwise direction of the line and that reflect the line-focused laser light L, an irradiation optical system 2 that irradiates the sample 19 with the laser light L reflected by the DMD 7, and a photodetector 17 in which a plurality of channels for detecting light from the sample 19 are arrayed in one column, wherein the DMD 7 is driven so that a plurality of movable micromirrors simultaneously reflect the laser light L and so that the movable micromirrors that reflect the laser light L are sequentially switched, and the stage 20 moves the sample 19 in a direction crossing the array direction of a plurality of light spots formed on the sample 19.
摘要翻译： 提供了一种激光扫描显微镜，其可以减少扫描和从高灵敏度的样品检测光所需的时间。 提供了一种激光扫描显微镜100，其包括移动放置在其上的样品19的载物台20，发射激光L的激光源1，将激光L聚焦成线状的线聚焦光学系统3，DMD 7包括沿线的长度方向排列并反射线聚焦激光L的多个可移动微镜;照射光学系统2，其用由DMD 7反射的激光L照射样品19;以及 其中用于检测来自样品19的光的多个通道被布置在一列中的光电检测器17，其中驱动DMD 7，使得多个可移动微镜同时反射激光L，并且使得反射 激光L依次切换，台20沿着与样品19上形成的多个光点的排列方向交叉的方向移动样品19。
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24.发明授权公开(公告)号：US07838818B2
公开(公告)日：2010-11-23
申请号：US11811792
申请日：2007-06-12
申请人： Shigeru Kanegae , Tatsuo Nakata
发明人： Shigeru Kanegae , Tatsuo Nakata
CPC分类号： G01N21/6458
摘要： The invention provides a light-stimulus illumination apparatus comprising a light source for emitting light-stimulus laser light; a scanning unit including at least one acousto-optic device for scanning the light-stimulus laser light emitted from the light source in a direction intersecting an optical axis; and a control unit for controlling the scanning unit. The control unit controls the scanning unit so that the light-stimulus laser light irradiates a plurality of spatially separated regions in a time-division manner.
摘要翻译： 本发明提供了一种光刺激照明装置，包括用于发射光激励激光的光源; 扫描单元，包括至少一个声光装置，用于沿与光轴相交的方向扫描从光源发出的光激励激光; 以及用于控制扫描单元的控制单元。 控制单元控制扫描单元，使得激光激光以时分方式照射多个空间分离的区域。
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公开(公告)号：US07696996B2
公开(公告)日：2010-04-13
申请号：US11294689
申请日：2005-12-05
IPC分类号： G06T11/20
CPC分类号： G02B21/002 , G02B21/365
摘要： A laser scanning microscope capable of quickly and accurately setting control values of control items for a microscope apparatus is provided. The control items and a time line are displayed along a vertical axis and a horizontal axis, respectively. The laser scanning microscope includes a graphical user interface configured to set the control values of the control items along the time line and a control unit configured to acquire luminance information of a specimen by irradiating the specimen with a laser beam in accordance with the control values set by the graphical user interface.
摘要翻译： 提供能够快速准确地设定显微镜装置的控制项的控制值的激光扫描显微镜。 控制项目和时间线分别沿垂直轴和水平轴显示。 激光扫描显微镜包括图形用户界面，被配置为沿着时间线设置控制项的控制值;以及控制单元，被配置为通过根据控制值设定用激光束照射样本来获取样本的亮度信息 通过图形用户界面。
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公开(公告)号：US07369308B2
公开(公告)日：2008-05-06
申请号：US11050883
申请日：2005-02-04
IPC分类号： G02B21/06
CPC分类号： G01N21/648 , G01N21/6458 , G02B21/0088 , G02B21/16
摘要： A total internal reflection fluorescence microscope includes a light source which generates laser light having a plurality of wavelengths, a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination, a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination, an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen, and a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched.
摘要翻译： 全内反射荧光显微镜包括产生具有多个波长的激光的光源，通过物镜以预定的入射角将来自光源的激光照射在样本上并产生ev逝照明的聚焦光学系统 观察由于ev逝照明而产生的荧光的荧光观察单元，调整照射在样本上的激光的入射角的入射角度调节器，以及控制入射角度调节器的控制器， 当切换来自激光光源的激光的波长时，渐逝光的透过深度相同。
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公开(公告)号：US20070263282A1
公开(公告)日：2007-11-15
申请号：US11799196
申请日：2007-05-01
申请人： Nobuhiro Takamizawa , Tatsuo Nakata
发明人： Nobuhiro Takamizawa , Tatsuo Nakata
IPC分类号： G02B21/00
CPC分类号： G02B21/0076 , G02B21/0032 , G02B21/006
摘要： An observation laser beam for observing a specimen and a manipulation laser beam for manipulating the specimen are multiplexed; the multiplexed beams irradiate the specimen, which is mounted on a stage, via an objective lens; and fluorescence emitted from inside the specimen in the observation optical axis direction is detected. In a preparation mode, a focal-position adjusting unit is controlled so that the focal position of the observation laser beam and the focal position of the manipulation laser beam are coincident, independent of the movement of the objective lens or the stage by a focusing mechanism; and in an observation mode, the focal-position adjusting unit is controlled so as to cancel out the shift of the focal position of the manipulation laser beam according to the movement of the objective lens or the stage.
摘要翻译： 用于观察样本的观察激光束和用于操纵样本的操纵激光束被多路复用; 复用光束经由物镜照射安装在台架上的试样; 并且在观察光轴方向检测从样本内部发出的荧光。 在准备模式中，控制焦点位置调节单元，使得观察激光束的焦点位置和操纵激光束的焦点位置一致，而与物镜或舞台的移动无关，其中聚焦机构 ; 并且在观察模式中，控制焦点位置调整单元，以便根据物镜或舞台的移动抵消操纵激光束的焦点位置的偏移。
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公开(公告)号：US07215469B2
公开(公告)日：2007-05-08
申请号：US11362473
申请日：2006-02-24
申请人： Tatsuo Nakata , Masahiro Aoki
发明人： Tatsuo Nakata , Masahiro Aoki
CPC分类号： G02B21/16 , G02B21/0068 , G02B21/0076
摘要： A confocal microscope comprises a light source emitting a polarized light beam, an objective lens irradiating the polarized light beam, which is deflected and scanned by the optical scanner, to the sample as an excitation light beam, a wavelength separator detecting a necessary wavelength band from a polarized fluorescence emitted from the sample which is excited by the polarized light beam, and a photodetector unit having a polarization property extractor extracting a fluorescence with a predetermined polarization property from the fluorescence detected with the wavelength separator, a wavelength selector selecting a wavelength of the fluorescence extracted by the polarization property extractor, and a photodetector detecting the fluorescence selected by the wavelength selector.
摘要翻译： 共聚焦显微镜包括发射偏振光束的光源，照射由光学扫描器偏转和扫描的偏振光束的物镜作为激发光束的样品，从波长分离器检测必要的波长带 从由所述偏振光束激发的样品发射的偏振荧光;以及光电检测器单元，具有偏振特性提取器，从由所述波长分离器检测到的荧光提取具有预定偏振特性的荧光;波长选择器， 由偏振特性提取器提取的荧光，以及检测由波长选择器选择的荧光的光检测器。
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公开(公告)号：US20060129353A1
公开(公告)日：2006-06-15
申请号：US11294689
申请日：2005-12-05
IPC分类号： G21C17/00
CPC分类号： G02B21/002 , G02B21/365
摘要： A laser scanning microscope capable of quickly and accurately setting control values of control items for a microscope apparatus is provided. The control items and a time line are displayed along a vertical axis and a horizontal axis, respectively. The laser scanning microscope includes a graphical user interface configured to set the control values of the control items along the time line and a control unit configured to acquire luminance information of a specimen by irradiating the specimen with a laser beam in accordance with the control values set by the graphical user interface.
摘要翻译： 提供能够快速准确地设定显微镜装置的控制项的控制值的激光扫描显微镜。 控制项目和时间线分别沿垂直轴和水平轴显示。 激光扫描显微镜包括图形用户界面，被配置为沿着时间线设置控制项的控制值;以及控制单元，被配置为通过根据控制值设定用激光束照射样本来获取样本的亮度信息 通过图形用户界面。
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公开(公告)号：US20050279950A1
公开(公告)日：2005-12-22
申请号：US11070191
申请日：2005-03-03
CPC分类号： G01N21/6486 , G01N21/6456
摘要： The invention provides a multiphoton-excitation-type examination apparatus that efficiently generates a multiphoton-excitation effect, that makes the measurement head compact, and that can be easily adjusted when the measurement head is replaced. The multiphoton-excitation-type examination apparatus comprises a laser light source that oscillates ultrashort pulsed laser light; an optical fiber that transmits the ultrashort pulsed laser light from the laser light source; a support member; a measurement head supported on the support member so as to be movable upwards and downwards and at an angle, and having an optical system that irradiates a specimen with the ultrashort pulsed laser light transmitted by the optical fiber that measures fluorescence or reflected light coming from the specimen; and a dispersion-compensating member, in the measurement head, that compensates for group velocity dispersion of the ultrashort pulsed laser light irradiated onto the specimen.
摘要翻译： 本发明提供了一种多光子激发型检查装置，其有效地产生使得测量头紧凑，并且在更换测量头时可以容易地调节的多光子激发效果。 多光子激发型检查装置包括：振荡超短脉冲激光的激光光源; 从激光光源发射超短脉冲激光的光纤; 支撑构件; 测量头支撑在支撑构件上，以便可上下移动一定角度，并且具有光学系统，该光学系统用由光纤透射的超短脉冲激光照射样本，该光纤测量荧光或来自 标本; 以及在测量头中补偿照射到样本上的超短脉冲激光的组速度色散的色散补偿部件。
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