公开(公告)号：US20230189795A1

公开(公告)日：2023-06-22

申请号：US18107930

申请日：2023-02-09

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Gabriel Nastase ,  Gideon Ukpai

IPC: A01N1/02

CPC classification number: A01N1/0284 ,  A01N1/0257 ,  A01N1/0221 ,  A01N1/0268 ,  A01N1/0289 ,  B01L2300/1894

Abstract: The disclosure provides processes for temperature and pressure controlled cryopreservation of samples by using isochoric systems.

公开(公告)号：US20220016827A1

公开(公告)日：2022-01-20

申请号：US17459717

申请日：2021-08-27

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Boris Rubinsky ,  Michal Adamkievicz ,  Ze'ev Shaked

IPC: B29C64/118 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y50/02 ,  B29C64/364 ,  B33Y40/00 ,  B33Y70/00 ,  B29C64/379 ,  B29C64/209 ,  B29C64/393 ,  B29C64/25 ,  B29C64/371 ,  C12M3/00 ,  C12N5/00

Abstract: Systems, apparatus and methods for producing objects with cryogenic 3D printing with controllable micro and macrostructure with potential applications in tissue engineering, drug delivery, and the food industry. The technology can produce complex structures with controlled morphology when the printed 3D object is immersed in a liquid coolant, whose upper surface is maintained at the same level as the highest deposited layer of the object. This ensures that the computer-controlled process of freezing is controlled precisely and already printed frozen layers remain at a constant temperature. The technology controls the temperature, flow rate and volume of the printed fluid emitted by the dispenser that has X-Y positional translation and conditions at the interface between the dispenser and coolant surface. The technology can also control the temperature of the pool of liquid coolant and the vertical position of the printing surface and pool of coolant liquid.

公开(公告)号：US20200178518A1

公开(公告)日：2020-06-11

申请号：US16637735

申请日：2018-08-09

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Gabriel Nastase ,  Gideon Ukpai

IPC: A01N1/02

Abstract: The disclosure provides processes for temperature and pressure controlled cryopreservation of samples by using isochoric systems.

公开(公告)号：US20190125209A1

公开(公告)日：2019-05-02

申请号：US16175100

申请日：2018-10-30

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Cesar A. GONZALEZ

IPC: A61B5/053 ,  A61B5/00 ,  A61B5/05 ,  A61B5/0205 ,  G01R33/483 ,  G01R33/34 ,  G01R33/36 ,  G01R33/48 ,  A61B5/02 ,  G01N27/02

Abstract: A method and apparatus of determining the condition of a bulk tissue sample, by: positioning a bulk tissue sample between a pair of induction coils (or antennae); passing a spectrum of alternating current (or voltage) through a first of the induction coils (or antennae); measuring spectrum of alternating current (or voltage) produced in the second of the induction coils (or antennae); and comparing the phase shift between the spectrum of alternating currents (or voltages) in the first and second induction coils (or antennae), thereby determining the condition of the bulk tissue sample.

公开(公告)号：US20180304537A1

公开(公告)日：2018-10-25

申请号：US15950106

申请日：2018-04-10

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Boris Rubinsky ,  Michal Adamkievicz ,  Ze'ev Shaked

IPC: B29C64/371 ,  B33Y30/00 ,  B33Y50/02 ,  B33Y10/00 ,  B29C64/393 ,  B33Y70/00 ,  B33Y40/00 ,  B29C64/209 ,  B29C64/25 ,  B29C64/379 ,  C12N5/00 ,  C12M3/00

CPC classification number: B29C64/371 ,  B29C64/106 ,  B29C64/20 ,  B29C64/209 ,  B29C64/25 ,  B29C64/379 ,  B29C64/386 ,  B29C64/393 ,  B29K2005/00 ,  B29K2089/00 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y40/00 ,  B33Y50/02 ,  B33Y70/00 ,  C12M21/08 ,  C12N5/0062 ,  C12N2513/00

Abstract: Systems, apparatus and methods for producing objects with cryogenic 3D printing with controllable micro and macrostructure with potential applications in tissue engineering, drug delivery, and the food industry. The technology can produce complex structures with controlled morphology when the printed 3D object is immersed in a liquid coolant, whose upper surface is maintained at the same level as the highest deposited layer of the object. This ensures that the computer-controlled process of freezing is controlled precisely and already printed frozen layers remain at a constant temperature. The technology controls the temperature, flow rate and volume of the printed fluid emitted by the dispenser that has X-Y positional translation and conditions at the interface between the dispenser and coolant surface. The technology can also control the temperature of the pool of liquid coolant and the vertical position of the printing surface and pool of coolant liquid.

公开(公告)号：US20140176158A1

公开(公告)日：2014-06-26

申请号：US14109647

申请日：2013-12-17

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Cesar A. Gonzalez

IPC: G01N27/02

CPC classification number: A61B5/0537 ,  A61B5/02042 ,  A61B5/0205 ,  A61B5/05 ,  A61B5/053 ,  A61B5/4064 ,  A61B5/413 ,  A61B5/4244 ,  A61B5/443 ,  A61B5/4519 ,  A61B5/4869 ,  A61B5/4878 ,  A61B5/7225 ,  A61B5/7228 ,  A61B5/725 ,  A61B2562/143 ,  G01N27/026 ,  G01R33/34 ,  G01R33/36 ,  G01R33/4828 ,  G01R33/483

Abstract: A method and apparatus of determining the condition of a bulk tissue sample, by: positioning a bulk tissue sample between a pair of induction coils (or antennae); passing a spectrum of alternating current (or voltage) through a first of the induction coils (or antennae); measuring spectrum of alternating current (or voltage) produced in the second of the induction coils (or antennae); and comparing the phase shift between the spectrum of alternating currents (or voltages) in the first and second induction coils (or antennae), thereby determining the condition of the bulk tissue sample.

Abstract translation: 通过以下步骤确定体组织样本的状况的方法和装置：将体组织样本定位在一对感应线圈（或天线）之间; 通过感应线圈（或天线）中的第一个通过交流（或电压）的频谱; 测量第二个感应线圈（或天线）中产生的交流（或电压）的频谱; 以及比较第一和第二感应线圈（或天线）中的交流电流（或电压）的频谱之间的相移，由此确定本体组织样本的状况。