公开(公告)号：US20180206760A1

公开(公告)日：2018-07-26

申请号：US15875660

申请日：2018-01-19

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Cesar A. GONZALEZ

IPC: A61B5/053 ,  A61B5/00 ,  A61B5/02

CPC classification number: A61B5/0537 ,  A61B5/02042 ,  A61B5/0205 ,  A61B5/05 ,  A61B5/053 ,  A61B5/4064 ,  A61B5/413 ,  A61B5/4244 ,  A61B5/443 ,  A61B5/4519 ,  A61B5/4869 ,  A61B5/4878 ,  A61B5/7225 ,  A61B5/7228 ,  A61B5/725 ,  A61B2562/143 ,  G01N27/026 ,  G01R33/34 ,  G01R33/36 ,  G01R33/4828 ,  G01R33/483

Abstract: A method and apparatus of determining the condition of a bulk tissue sample, by: positioning a bulk tissue sample between a pair of induction coils (or antennae); passing a spectrum of alternating current (or voltage) through a first of the induction coils (or antennae); measuring spectrum of alternating current (or voltage) produced in the second of the induction coils (or antennae); and comparing the phase shift between the spectrum of alternating currents (or voltages) in the first and second induction coils (or antennae), thereby determining the condition of the bulk tissue sample.

公开(公告)号：US20160287313A1

公开(公告)日：2016-10-06

申请号：US14679449

申请日：2015-04-06

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Charlotte Daniels

IPC: A61B18/14

CPC classification number: A61B18/14 ,  A61B2018/00613 ,  A61N1/0412 ,  A61N1/327

Abstract: Electrical pulses are applied to tissue in a manner which destroys targeted cells such as cancerous cells while sparing non-targeted cells such as nerve cells. The electrical pulses are controlled within ranges for voltage, wattage and duration of application. Multiple pulses or groups of pulses may be applied to obtain a desired result while maintaining any temperature increase below a level which destroys cells.

Abstract translation: 电脉冲以破坏诸如癌细胞的靶细胞的方式施用于组织，同时保留非靶向细胞例如神经细胞。 电脉冲控制在电压，瓦数和应用持续时间的范围内。 可以施加多个脉冲或脉冲组以获得期望的结果，同时保持任何温度升高低于破坏细胞的水平。

公开(公告)号：US08633033B2

公开(公告)日：2014-01-21

申请号：US13723696

申请日：2012-12-21

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Cesar A. Gonzalez

IPC: G01N27/00 ,  G01N33/48 ,  G01R27/08 ,  G01R25/00 ,  A61B5/053

CPC classification number: A61B5/0537 ,  A61B5/02042 ,  A61B5/0205 ,  A61B5/05 ,  A61B5/053 ,  A61B5/4064 ,  A61B5/413 ,  A61B5/4244 ,  A61B5/443 ,  A61B5/4519 ,  A61B5/4869 ,  A61B5/4878 ,  A61B5/7225 ,  A61B5/7228 ,  A61B5/725 ,  A61B2562/143 ,  G01N27/026 ,  G01R33/34 ,  G01R33/36 ,  G01R33/4828 ,  G01R33/483

Abstract: A method and apparatus of determining the condition of a bulk tissue sample, by: positioning a bulk tissue sample between a pair of induction coils (or antennae); passing a spectrum of alternating current (or voltage) through a first of the induction coils (or antennae); measuring spectrum of alternating current (or voltage) produced in the second of the induction coils (or antennae); and comparing the phase shift between the spectrum of alternating currents (or voltages) in the first and second induction coils (or antennae), thereby determining the condition of the bulk tissue sample.

Abstract translation: 通过以下步骤确定体组织样本的状况的方法和装置：将体组织样本定位在一对感应线圈（或天线）之间; 通过感应线圈（或天线）中的第一个通过交流（或电压）的频谱; 测量第二个感应线圈（或天线）中产生的交流（或电压）的频谱; 以及比较第一和第二感应线圈（或天线）中的交流电流（或电压）的频谱之间的相移，由此确定本体组织样本的状况。

公开(公告)号：US20220325937A1

公开(公告)日：2022-10-13

申请号：US17845114

申请日：2022-06-21

Applicant: The Regents of the University of California

Inventor： Matthew J. Powell-Palm ,  Boris Rubinsky

IPC: F25D3/08

Abstract: Supercooled water or solutions can be destabilized by the nucleation of ice, which can be triggered by a wide range of different mechanisms. Constraining water in a constant-volume (isochoric) container minimizes the effects of many of these mechanisms through thermodynamic, kinetic, and fluid dynamic means, significantly enhancing supercooling stability. This invention describes methods and devices for high-stability and optionally transportable supercooling of aqueous solutions or suspensions, and the stable and long-term preservation of biological matter that may be stored therein.

公开(公告)号：US11130277B2

公开(公告)日：2021-09-28

申请号：US15950106

申请日：2018-04-10

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Boris Rubinsky ,  Michal Adamkievicz ,  Ze'Ev Shaked

IPC: B29C64/118 ,  B33Y10/00 ,  B33Y50/02 ,  B29C64/364 ,  B33Y40/00 ,  B33Y70/00 ,  B29C64/393 ,  B29C64/371 ,  C12N5/00 ,  B33Y30/00 ,  B29C64/379 ,  B29C64/209 ,  B29C64/25 ,  C12M3/00

Abstract: Systems, apparatus and methods for producing objects with cryogenic 3D printing with controllable micro and macrostructure with potential applications in tissue engineering, drug delivery, and the food industry. The technology can produce complex structures with controlled morphology when the printed 3D object is immersed in a liquid coolant, whose upper surface is maintained at the same level as the highest deposited layer of the object. This ensures that the computer-controlled process of freezing is controlled precisely and already printed frozen layers remain at a constant temperature. The technology controls the temperature, flow rate and volume of the printed fluid emitted by the dispenser that has X-Y positional translation and conditions at the interface between the dispenser and coolant surface. The technology can also control the temperature of the pool of liquid coolant and the vertical position of the printing surface and pool of coolant liquid.

公开(公告)号：US11033321B2

公开(公告)日：2021-06-15

申请号：US16160205

申请日：2018-10-15

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Rafael Davalos ,  Boris Rubinsky

IPC: A61B18/12 ,  A61N1/04 ,  A61B18/14 ,  A61N1/05 ,  A61N1/32 ,  A61B18/00

Abstract: A new method for the ablation of undesirable tissue such as cells of a cancerous or non-cancerous tumor is disclosed. It involves the placement of electrodes into or near the vicinity of the undesirable tissue through the application of electrical pulses causing irreversible electroporation of the cells throughout the entire area of the undesirable tissue. The electric pulses irreversibly permeate the cell membranes, thereby invoking cell death. The irreversibly permeabilized cells are left in situ and are removed by the body immune system. The amount of tissue ablation achievable through the use of irreversible electroporation without inducing thermal damage is considerable.

公开(公告)号：US10668208B2

公开(公告)日：2020-06-02

申请号：US15988408

申请日：2018-05-24

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  ANGIODYNAMICS, INC.

Inventor： Boris Rubinsky ,  Elad Maor ,  Antoni Ivorra ,  James J. Mitchell ,  William C. Hamilton

IPC: A61N1/32 ,  A61B18/14 ,  A61M5/14 ,  A61B17/22 ,  A61B18/00

Abstract: Restenosis or neointimal formation may occur following angioplasty or other trauma to an artery such as by-pass surgery. This presents a major clinical problem which narrows the artery. The invention provides a balloon catheter with a particular electrode configuration. Also provided is a method whereby vascular cells in the area of the artery subjected to the trauma are subjected to irreversible electroporation which is a non-thermal, non-pharmaceutical method of applying electrical pulses to the cells so that substantially all of the cells in the area are ablated while leaving the structure of the vessel in place and substantially unharmed due to the non-thermal nature of the procedure.

公开(公告)号：US10143512B2

公开(公告)日：2018-12-04

申请号：US14679449

申请日：2015-04-06

Applicant: The Regents of the University of California

Inventor： Boris Rubinsky ,  Charlotte Daniels

IPC: A61B18/14 ,  A61N1/04 ,  A61N1/32 ,  A61B18/00

Abstract: Electrical pulses are applied to tissue in a manner which destroys targeted cells such as cancerous cells while sparing non-targeted cells such as nerve cells. The electrical pulses are controlled within ranges for voltage, wattage and duration of application. Multiple pulses or groups of pulses may be applied to obtain a desired result while maintaining any temperature increase below a level which destroys cells.

公开(公告)号：US10117701B2

公开(公告)日：2018-11-06

申请号：US14639632

申请日：2015-03-05

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Rafael Davalos ,  Boris Rubinsky

IPC: A61B18/12 ,  A61N1/04 ,  A61B18/14 ,  A61N1/05 ,  A61N1/32 ,  A61B18/00

Abstract: A new method for the ablation of undesirable tissue such as cells of a cancerous or non-cancerous tumor is disclosed. It involves the placement of electrodes into or near the vicinity of the undesirable tissue through the application of electrical pulses causing irreversible electroporation of the cells throughout the entire area of the undesirable tissue. The electric pulses irreversibly permeate the cell membranes, thereby invoking cell death. The irreversibly permeabilized cells are left in situ and are removed by the body immune system. The amount of tissue ablation achievable through the use of irreversible electroporation without inducing thermal damage is considerable.

公开(公告)号：US10010666B2

公开(公告)日：2018-07-03

申请号：US14313647

申请日：2014-06-24

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  ANGIODYNAMICS, INC.

Inventor： Boris Rubinsky ,  Elad Maor ,  Antoni Ivorra ,  James J. Mitchell ,  William C. Hamilton

IPC: A61M5/14 ,  A61B18/14 ,  A61N1/32 ,  A61B17/22 ,  A61B18/00

CPC classification number: A61M5/14 ,  A61B18/14 ,  A61B18/1492 ,  A61B2017/22038 ,  A61B2018/00166 ,  A61B2018/00214 ,  A61B2018/0022 ,  A61B2018/0041 ,  A61B2018/00613 ,  A61B2018/1435 ,  A61N1/327

Abstract: Restenosis or neointimal formation may occur following angioplasty or other trauma to an artery such as by-pass surgery. This presents a major clinical problem which narrows the artery. The invention provides a balloon catheter with a particular electrode configuration. Also provided is a method whereby vascular cells in the area of the artery subjected to the trauma are subjected to irreversible electroporation which is a non-thermal, non-pharmaceutical method of applying electrical pulses to the cells so that substantially all of the cells in the area are ablated while leaving the structure of the vessel in place and substantially unharmed due to the non-thermal nature of the procedure.