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    Clonal expansion of T cells of unknown specificity and identification of ligand recognized by the clonally expanded T cells
    24.
    发明授权
    Clonal expansion of T cells of unknown specificity and identification of ligand recognized by the clonally expanded T cells 有权
    未知特异性T细胞的克隆扩增和克隆扩增的T细胞识别的配体的鉴定

    公开(公告)号：US07132243B2

    公开(公告)日：2006-11-07

    申请号：US09962445

    申请日：2001-09-26

    申请人： Sho Matsushita ,  Toshihiro Nakashima

    发明人： Sho Matsushita ,  Toshihiro Nakashima

    IPC分类号： G01N33/53 ,  G01N33/566 ,  C12N5/08

    CPC分类号： C12N5/0636 ,  C12N2501/23 ,  C12N2501/58 ,  C12N2503/00 ,  G01N33/505

    摘要： A process for clonally expanding T cells of unknown specificity and a process for identifying a peptide ligand recognized by said T cells are provided.A process for clonally expanding T cells of unknown specificity which comprises co-culturing said T cells of unknown specificity with combinatorial randomized peptide library (Xn peptide library wherein n is a number of amino acids) consisted of peptides having a randomized amino acid sequence consisted of amino acid residues selected from nineteen kinds of naturally occurring amino acid other than cysteine, an interleukin, and major histocompatibility complex (MHC) class II antigen-expressing cells with DNA synthesis being suspended, said cells being derived from the individual where said T cells are obtained; and a process for identifying a peptide ligand recognized by the T cell clone of unknown specificity obtained by said process, which comprises determining proliferating activity of said T cell clone with Xn peptide library, in the presence of an interleukin and MHC class II antigen-expressing cells with DNA synthesis being suspended, said cells being derived from the individual where said T cells are obtained, to thereby determine a peptide sequence that can activate proliferation of T cells.

    摘要翻译： 提供了用于克隆扩增未知特异性的T细胞的方法和用于鉴定所述T细胞识别的肽配体的方法。 一种用于克隆扩增未知特异性的T细胞的方法，其包括由具有随机化氨基酸序列的肽组成的组合随机肽文库（Xn肽文库，其中n为多个氨基酸）共同培养所述具有未知特异性的T细胞 选自十九种除了半胱氨酸之外的天然存在的氨基酸，白细胞介素和悬浮有DNA合成的主要组织相容性复合体（MHC）II类抗原表达细胞的氨基酸残基，所述细胞衍生自所述T细胞的个体 获得; 以及用于鉴定由所述方法获得的未知特异性的T细胞克隆识别的肽配体的方法，其包括在存在白介素和MHC II类抗原表达的情况下，用Xn肽文库测定所述T细胞克隆的增殖活性 悬浮有DNA合成的细胞，所述细胞衍生自获得所述T细胞的个体，从而确定可以激活T细胞增殖的肽序列。

    Data transmission confirmation by sending frame with command to change transmitter's resend counter when receiver's buffer is full
    30.
    发明授权
    Data transmission confirmation by sending frame with command to change transmitter's resend counter when receiver's buffer is full 失效
    通过发送帧的数据传输确认，当接收机的缓冲区已满时，用命令改变发射机的重发计数器

    公开(公告)号：US5623602A

    公开(公告)日：1997-04-22

    申请号：US44485

    申请日：1993-04-09

    申请人： Toshihiro Nakashima

    发明人： Toshihiro Nakashima

    IPC分类号： G06F13/00 ,  H04L12/28 ,  H04L29/06 ,  H04L29/08 ,  G06F13/12 ,  G06F13/42

    CPC分类号： H04L47/10 ,  H04L29/06 ,  H04L47/32 ,  H04L69/324

    摘要： A computer network system includes a plurality of computers and a network connected to the computers for allowing them to interchange data. Each computer has a data send module and a data receive module. The data send module is made up of a means for sending a data frame to the network in response to a send request, a resend number storing means for storing the number of times that the data frame is to be resent, and a means for updating the content of the resend number storing means upon receiving a frame for changing the number of times that the data frame is to be resent from the network. The data receive module includes a receive buffer for storing the data frame received from the network, and a means for sending, when the receive buffer is full, the frame for changing the number of times that the data frame is to be resent to the network.

    摘要翻译： 计算机网络系统包括多个计算机和连接到计算机以允许它们互换数据的网络。 每台计算机都有一个数据发送模块和一个数据接收模块。 数据发送模块由用于响应于发送请求向网络发送数据帧的装置，用于存储要重发数据帧的次数的重发数量存储装置和用于更新的装置 在接收到用于改变要从网络重新发送数据帧的次数的帧时，重发数存储装置的内容。 数据接收模块包括用于存储从网络接收的数据帧的接收缓冲器，以及用于当接收缓冲器满时发送用于将数据帧重新发送到网络的次数发送的帧的装置 。