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公开(公告)号：US11747310B2
公开(公告)日：2023-09-05
申请号：US16664403
申请日：2019-10-25
IPC分类号： G01N30/00 , G01N30/02 , B01D15/30 , C07D215/48 , G01N30/04 , C07H15/26 , G01N30/88 , G01N33/68 , G01N30/62
CPC分类号： G01N30/02 , B01D15/305 , C07D215/48 , C07H15/26 , G01N30/04 , G01N30/88 , G01N33/6842 , G01N2030/027 , G01N2030/047 , G01N2030/626 , G01N2030/8836 , G01N2400/00 , G01N2440/38
摘要： Methods are provided for making rapid labeled dextran ladders and other calibrants useful in liquid chromatography. The methodologies include a two-step process comprising a reductive amination step of providing a reducing glycan and reacting it with a compound having a primary amine to produce an intermediate compound. The intermediate compound is then rapidly tagged with a rapid tagging reagent to produce the rapid labeled dextran ladder.
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公开(公告)号：US20230136953A1
公开(公告)日：2023-05-04
申请号：US17976030
申请日：2022-10-28
IPC分类号： G01N33/53 , G01N33/92 , C12Q1/6806
摘要： The present disclosure provides compositions and methods for sample processing, particularly for oligonucleotide analysis e.g. analysis of formulated nucleic acid drugs. A composition for pretreating at least one target nucleic acid in a biological mixture provided herein includes a chaotropic agent selected from a substituted guanidine, a substituted amidine, a substituted quaternary amine, or a combination thereof, an optional protease, and/or an optional disulfide-reducing agent. Methods of analyzing at least one target nucleic acid in a biological mixture is also provided herein. Furthermore, the present disclosure provides methods for quantifying at least one target cationic lipid interacting with a nucleic acid.
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公开(公告)号：US20230126856A1
公开(公告)日：2023-04-27
申请号：US17970962
申请日：2022-10-21
发明人： Matthew A. Lauber , Darryl W. Brousmiche , Jun Yang , Weiqiang Gu , Mingcheng Xu , Peng Chen
IPC分类号： G01N33/68 , C07D207/46
摘要： Provided herein chiral derivatization reagents for use in separating and detecting of amine containing enantiomers. The said chiral derivatization reagents provide a combination of improved detectable properties to facilitate various downstream analyses. In particular, the chiral derivatization reagents include at least one chiral carbon atom; at least one strongly basic moiety; at least one chromophore moiety or at least one fluorophore moiety; and at least one reactive group. The present disclosure further provides methods for analyzing amine-containing enantiomeric isomers using a chromatographic separation device and a mass spectroscopy.
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公开(公告)号：US20230111049A1
公开(公告)日：2023-04-13
申请号：US17961061
申请日：2022-10-06
发明人： Matthew A. Lauber , Xiaoxiao Liu
IPC分类号： C12Q1/6872
摘要： Methods for quenching a nuclease digestion of a target nucleic acid prior to downstream analysis of the target nucleic acid are disclosed herein. Particularly, methods for controlling the end point of a nuclease digestion prior to sequence analysis of a target nucleic acid is provided. Quenching of a nuclease digestion in the present disclosure employs at least one non-ionic or anionic denaturant combined with an optional reducing agent. The methods presented in this disclosure aids preserving the sample comprising the target nucleic acid or fragments thereof for long term storage and ensures that the effect of contaminating nucleases is eliminated during pretreatment step.
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25.发明授权公开(公告)号：US11433320B2
公开(公告)日：2022-09-06
申请号：US16259705
申请日：2019-01-28
发明人： Matthew A. Lauber , Jennifer M. Nguyen , Ximo Zhang , Nilini Ranbaduge , Robert Birdsall , Henry Shion
IPC分类号： B01D15/42 , B01D15/32 , G01N30/34 , B01D15/16 , B01J20/283 , B01J20/287 , G01N30/72 , G01N30/84 , B01D15/30
摘要： The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 0.20% (v/v) difluoroacetic acid and less than about 100 ppb of any individual metal impurity. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.
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公开(公告)号：US20220080388A1
公开(公告)日：2022-03-17
申请号：US17477340
申请日：2021-09-16
发明人： Yeliz Tunc Sarisozen , Nicole L. Lawrence , Darryl W. Brousmiche , Andrew Wyatt Schmudlach , Matthew A. Lauber
IPC分类号： B01J20/289 , B01J20/26 , B01J20/28
摘要： The present disclosure is directed to stationary phase materials (e.g., porous inorganic-organic hybrid particles) for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified stationary phase materials.
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27.发明申请公开(公告)号：US20220080385A1
公开(公告)日：2022-03-17
申请号：US17477347
申请日：2021-09-16
摘要： The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.
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公开(公告)号：US20220034852A1
公开(公告)日：2022-02-03
申请号：US17370589
申请日：2021-07-08
IPC分类号： G01N30/06 , G01N30/72 , C08F230/08 , B01D15/30 , C08L33/14 , C08F220/54 , C08F220/56 , B01J20/28 , B01J20/32 , C08F222/38 , B01J20/286
摘要： The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.
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29.发明申请公开(公告)号：US20210348154A1
公开(公告)日：2021-11-11
申请号：US17314853
申请日：2021-05-07
IPC分类号： C12N11/06 , G01N33/543
摘要： The present disclosure relates to a hetero-functional coating applied on a solid support. The coating includes a first functionality for conjugating biomolecules for the analysis of a protein or nucleic acid, and a second functionality for preventing undesired interactions between analytes of interest and the surface of solid support.
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公开(公告)号：US20210245150A1
公开(公告)日：2021-08-12
申请号：US17166051
申请日：2021-02-03
发明人： Anna K. Boardman , Xiaoxiao Liu , Micah Watt , Jonathan Belanger , Matthew A. Lauber , Michael Lavallee , Wenhao Zhu
摘要： The present technology relates to a customizable sample preparation device (e.g., liquid sample preparation device, such as a purification, clean-up, or separation device). In particular, the present technology relates to customizable devices formed from modular segments tailored to address one or more of the following: to optimize different sample and elution volumes, to incorporate various connection mechanisms to liquid handlers, to incorporate various liquid dispensing flow conditions, and to fulfill broad applications through the selection of specific resin for sample preparation. Some embodiments are directed to affinity capture devices, such as for example, Protein A affinity capture devices, utilizing a polymethacrylate-based resin. Other resins as well as other materials for processing a liquid sample are described herein.
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