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公开(公告)号：US06800791B1
公开(公告)日：2004-10-05
申请号：US09570319
申请日：2000-05-12
申请人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
发明人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
IPC分类号： A01H500
CPC分类号： C12N15/62 , C07H21/04 , C07K2319/09 , C07K2319/10 , C07K2319/61 , C07K2319/80 , C12N15/8205
摘要： The invention relates to the use of a modified Agrobacterium to deliver proteins directly to plant cells. Proteins of interest are delivered to the plant host in the form of a fusion protein with the Agrobacterium virulence protein VirF. Nucleotide sequences encoding such fusion proteins of VirF and a protein of interest are provided. Also provided are bacteria modified to comprise such fusion proteins of VirF and a protein of interest. Methods of introducing such fusion proteins into a plant host are provided. The invention finds use in facilitating plant transformation and particularly in the bio-engineering of desirable traits into crop plants.
摘要翻译： 本发明涉及使用改良的农杆菌将蛋白质直接递送至植物细胞。 感兴趣的蛋白质以与农杆菌毒力蛋白VirF融合蛋白的形式递送至植物宿主。 提供编码VirF和感兴趣蛋白质的融合蛋白的核苷酸序列。 还提供了细菌修饰以包含VirF和感兴趣的蛋白质的这种融合蛋白。 提供将这种融合蛋白引入植物宿主的方法。本发明用于促进植物转化，特别是在对作物植物期望的性状的生物工程中。
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22.发明申请AP2 DOMAIN TRANSCRIPTION FACTOR ODP2 (OVULE DEVELOPMENT PROTEIN 2) AND METHODS OF USE 有权AP2域转录因子ODP2（OVULE DEVELOPMENT蛋白2）及其使用方法公开(公告)号：US20110010795A9
公开(公告)日：2011-01-13
申请号：US12503482
申请日：2009-07-15
申请人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
发明人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
CPC分类号： C12N15/8243 , A01H1/08 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8223 , C12N15/8231 , C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译： 提供了调节植物发育的方法和组合物。 提供编码Ovule Development Protein 2（ODP2）蛋白的核苷酸序列和氨基酸序列。 这些序列可以用于各种方法，包括调节发育，发育途径，改变植物中的油含量，提高转化效率，调节胁迫耐受性和调节植物的再生能力。 还提供了转化的植物，植物细胞，组织和种子。
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公开(公告)号：US07799566B2
公开(公告)日：2010-09-21
申请号：US11560550
申请日：2006-11-16
申请人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
发明人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
CPC分类号： C12N15/8209 , C07K14/415 , C12N15/8201 , C12N15/8206 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated polynucleotides, specifically Cyclin D polynucleotides, and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants for the purpose of increasing transformation efficiency.
摘要翻译： 本发明提供分离的多核苷酸，特别是细胞周期蛋白D多核苷酸及其编码的参与细胞周期调节的蛋白质。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了改变植物的细胞周期蛋白质含量和/或组成以提高转化效率的方法和组合物。
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公开(公告)号：US20100100981A1
公开(公告)日：2010-04-22
申请号：US12018292
申请日：2008-01-23
申请人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
发明人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。
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25.发明授权AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use 有权AP2结构域转录因子ODP2（胚珠发育蛋白2）及其使用方法公开(公告)号：US07579529B2
公开(公告)日：2009-08-25
申请号：US11045802
申请日：2005-01-28
申请人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
发明人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
CPC分类号： C12N15/8243 , A01H1/08 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8223 , C12N15/8231 , C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译： 提供了调节植物发育的方法和组合物。 提供编码Ovule Development Protein 2（ODP2）蛋白的核苷酸序列和氨基酸序列。 这些序列可以用于各种方法，包括调节发育，发育途径，改变植物中的油含量，提高转化效率，调节胁迫耐受性和调节植物的再生能力。 还提供了转化的植物，植物细胞，组织和种子。
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公开(公告)号：US07220894B2
公开(公告)日：2007-05-22
申请号：US10744149
申请日：2003-12-22
申请人： Keith S. Lowe , William J. Gordon-Kamm , Theodore M. Klein , Sonriza Rasco-Gaunt , Xifan Sun , George J. Hoerster
发明人： Keith S. Lowe , William J. Gordon-Kamm , Theodore M. Klein , Sonriza Rasco-Gaunt , Xifan Sun , George J. Hoerster
CPC分类号： C07K14/415 , C12N15/8209 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： The invention provides methods of transforming plants and plant cells including recalcitrant cells with isolated nucleic acids encoding LEC1 transcriptional activators to induce transformation or regeneration efficiency, apomixis or somatic embryogenesis.
摘要翻译： 本发明提供了用分离的编码LEC1转录激活因子的核酸转化植物和植物细胞（包括顽固细胞）以诱导转化或再生效率，无融合生殖或体细胞胚发生的方法。
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公开(公告)号：US06933376B2
公开(公告)日：2005-08-23
申请号：US09496444
申请日：2000-02-02
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8206 , C07K14/415 , C12N15/8201 , C12N15/8207 , C12N15/8209 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated polynucleotides and their encoded proteins that are involved in cell cycle regulation. The invention further provides vectors, recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants.
摘要翻译： 本发明提供参与细胞周期调节的分离的多核苷酸及其编码的蛋白质。 本发明还提供载体，重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了关于改变细胞周期蛋白质含量和/或植物组成的方法和组合物。
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公开(公告)号：US06777590B2
公开(公告)日：2004-08-17
申请号：US09470526
申请日：1999-12-22
申请人： Yuejin Sun , Brian R. Dilkes , Brian A. Larkins , Keith S. Lowe , William J. Gordon-Kamm , Ricardo A. Dante
发明人： Yuejin Sun , Brian R. Dilkes , Brian A. Larkins , Keith S. Lowe , William J. Gordon-Kamm , Ricardo A. Dante
IPC分类号： C12N1529
CPC分类号： C12N9/1205 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8206 , C12N15/8209 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated nucleic acids and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content, cell cycle progression and/or composition of plants.
摘要翻译： 本发明提供分离的核酸及其编码的参与细胞周期调节的蛋白质。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了涉及改变细胞周期蛋白质含量，细胞周期进程和/或植物组成的方法和组合物。
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公开(公告)号：US06770800B2
公开(公告)日：2004-08-03
申请号：US09511445
申请日：2000-02-22
申请人： William J. Gordon-Kamm , Keith S. Lowe , Carolyn A. Gregory , George J. Hoerster , Brian A. Larkins , Brian R. Dilkes , Ronald Burnett
发明人： William J. Gordon-Kamm , Keith S. Lowe , Carolyn A. Gregory , George J. Hoerster , Brian A. Larkins , Brian R. Dilkes , Ronald Burnett
IPC分类号： C12N1582
CPC分类号： C12N9/127 , C07K14/005 , C12N15/8261 , C12N2750/12022 , Y02A40/146
摘要： The invention provides novel methods of using viral replicase polypeptides and polynucleotides. Included are methods for modulating endoreduplication and increasing crop yield.
摘要翻译： 本发明提供使用病毒复制酶多肽和多核苷酸的新方法。 包括调节内吞重复和提高作物产量的方法。
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公开(公告)号：US20110167522A1
公开(公告)日：2011-07-07
申请号：US12844965
申请日：2010-07-28
申请人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
发明人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
CPC分类号： C12N15/8209 , C07K14/415 , C12N15/8201 , C12N15/8206 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated polynucleotides, specifically Cyclin D polynucleotides, and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants for the purpose of increasing transformation efficiency.
摘要翻译： 本发明提供分离的多核苷酸，特别是细胞周期蛋白D多核苷酸及其编码的参与细胞周期调节的蛋白质。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了改变植物的细胞周期蛋白质含量和/或组成以提高转化效率的方法和组合物。
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