公开(公告)号：US08187804B2

公开(公告)日：2012-05-29

申请号：US10580989

申请日：2004-11-26

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N33/6896 ,  G01N33/9413 ,  G01N2800/2835

摘要： The present invention identified Lmx1a genes, which are expressed in dopaminergic neurons at all differentiation stages, from proliferating dopaminergic neuron progenitor cells before cell cycle exit to cells after cell cycle exit. Lmx1a expression in cells can be used as an indicator when selecting cells suitable for transplantation therapy for neurodegenerative diseases such as Parkinson's disease, and is useful as a marker for screening agents involved in the induction of dopaminergic neuron differentiation.

摘要翻译： 本发明鉴定了在细胞周期退出至细胞周期后出现的细胞周期性增殖的多巴胺能神经元祖细胞中，在所有分化阶段的多巴胺能神经元中表达的Lmx1a基因。 当选择适合于帕金森病等神经变性疾病的移植治疗的细胞时，细胞中的Lmx1a表达可用作指示剂，并且可用作参与多巴胺能神经元分化诱导的筛选剂的标记。

公开(公告)号：US20110201003A1

公开(公告)日：2011-08-18

申请号：US13057838

申请日：2009-08-06

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Eri Mizuhara

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Eri Mizuhara

IPC分类号： C12N5/0797 ,  G01N33/566 ,  C12Q1/68 ,  C07H21/00 ,  C07K16/18

CPC分类号： C12N5/0619 ,  C12N5/0623 ,  G01N33/56966

摘要： 65B13 was discovered to be a useful marker for isolating GABA neuron progenitor cells including Purkinje cells. Furthermore, E-cadherin was revealed to be a useful marker for isolating Purkinje progenitor cells from a 65B13-positive cell population. Specifically, when used in combination with 65B13, E-cadherin was found to be a useful marker for isolating Purkinje progenitor cells.

摘要翻译： 65B13被发现是分离包括浦肯野细胞在内的GABA神经元祖细胞的有用标记物。 此外，E-钙粘蛋白被揭示为从65B13阳性细胞群体中分离浦肯野祖细胞的有用标记物。 具体来说，当与65B13组合使用时，发现E-钙粘蛋白是用于分离浦肯野祖细胞的有用标记物。

公开(公告)号：US20070254281A1

公开(公告)日：2007-11-01

申请号：US10580989

申请日：2004-11-26

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N33/6896 ,  G01N33/9413 ,  G01N2800/2835

摘要： The present invention identified Lmx1a genes, which are expressed in dopaminergic neurons at all differentiation stages, from proliferating dopaminergic neuron progenitor cells before cell cycle exit to cells after cell cycle exit. Lmx1a expression in cells can be used as an indicator when selecting cells suitable for transplantation therapy for neurodegenerative diseases such as Parkinson's disease, and is useful as a marker for screening agents involved in the induction of dopaminergic neuron differentiation.

摘要翻译： 本发明鉴定了在细胞周期退出至细胞周期后出现的细胞周期性增殖的多巴胺能神经元祖细胞中，在所有分化阶段的多巴胺能神经元中表达的Lmx1a基因。 当选择适合于帕金森病等神经变性疾病的移植治疗的细胞时，细胞中的Lmx1a表达可用作指示剂，并且可用作参与多巴胺能神经元分化诱导的筛选剂的标记。

公开(公告)号：US20110256548A1

公开(公告)日：2011-10-20

申请号：US13141063

申请日：2009-12-18

申请人： Yuichi Ono ,  Tomoya Nakatani ,  Yasuko Nakagawa

发明人： Yuichi Ono ,  Tomoya Nakatani ,  Yasuko Nakagawa

IPC分类号： C12Q1/68 ,  C12N5/071 ,  G01N33/567

CPC分类号： C12N5/0678

摘要： The present invention provides markers that can selectively distinguish pancreatic progenitor cells. The present invention also provides methods for distinguishing pancreatic progenitor cells by using the markers as an indicator, and reagents to be used in the methods.The present inventors successfully identified the surface marker Nephrin-like 3 (Neph3) which is specifically expressed in pancreatic progenitor cells, and isolated pancreatic progenitor cells using the marker as an indicator or such. Viable pancreatic progenitor cells can be selected by using Neph3 as an indicator without using translated products and transcripts of any foreign genes. The marker is useful in preparing and identifying pancreatic progenitor cells which are applied to regenerative medicine or such for treatment of pancreatic diseases.

摘要翻译： 本发明提供了可以选择性区分胰腺祖细胞的标志物。 本发明还提供了通过使用标记作为指标来区分胰腺祖细胞的方法，以及用于该方法的试剂。 本发明人成功地鉴定了在胰腺祖细胞中特异性表达的表面标记物Nephrin样3（Neph3），以及使用该标记作为指示剂的分离的胰腺祖细胞。 可以通过使用Neph3作为指标来选择可行的胰腺祖细胞，而不使用翻译的产物和任何外来基因的转录物。 该标记物可用于制备和鉴定适用于再生医学或胰腺疾病治疗的胰腺祖细胞。

公开(公告)号：US20100303771A1

公开(公告)日：2010-12-02

申请号：US12524153

申请日：2008-02-07

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Eri Mizuhara

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Eri Mizuhara

IPC分类号： A61K35/30 ,  C12Q1/68 ,  G01N33/68 ,  G01N33/567 ,  C12N5/0797 ,  C07H21/00 ,  C07K16/18 ,  C07H21/04 ,  A61P25/00 ,  A61P25/28

CPC分类号： C07K16/40 ,  C07K14/47 ,  C07K16/18 ,  G01N33/5023 ,  G01N33/5058 ,  G01N33/5073 ,  G01N33/56966 ,  G01N33/9426

摘要： The present inventors identified a selective marker 65B13 for GABA neuron progenitor cells of the spinal dorsal horn and cerebellum, and successfully isolated GABA neuron progenitor cells using antibodies that bind to a protein encoded by the gene. 65B13 was demonstrated to be useful as a marker to isolate GABA-producing neuron progenitor cells in the spinal dorsal horn and cerebellum. GABA neuron progenitor cells can be efficiently identified or isolated by using the identified marker as an indicator.

摘要翻译： 本发明人鉴定了用于脊髓背角和小脑的GABA神经元祖细胞的选择性标记65B13，并且使用结合由该基因编码的蛋白质的抗体成功分离GABA神经元祖细胞。 65B13被证明可用作分离脊髓背角和小脑中产生GABA的神经元祖细胞的标志物。 通过使用鉴定的标记作为指标，可以有效地鉴定或分离GABA神经元祖细胞。

公开(公告)号：US20080213757A1

公开(公告)日：2008-09-04

申请号：US11574115

申请日：2005-08-23

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

IPC分类号： C12Q1/68 ,  G01N33/567

CPC分类号： C12Q1/6876 ,  C12Q2600/158 ,  G01N33/5026 ,  G01N33/5058 ,  G01N33/5073

摘要： As a result of screening for genes that are selectively expressed in fetal mouse brain region by subtraction method, the present inventors obtained a cDNA fragment encoding Corl1. The expression of Corl1 was examined by RT-PCR, in situ hybridization, and immunostaining using polyclonal antibodies. The results demonstrated that Corl1 was especially expressed at a high level of selectively in the central nervous system during embryonic stages. The expression patterns of Corl1 determined using various markers in embryonic spinal cord were compared to identify types of neurons expressing Corl1. The results revealed that Corl1 was specifically expressed in spinal cord interneurons dI4, dI5, dILA, and dILB. Accordingly, the present invention provides for discrimination between dI4 and dI6, neurons which previously could only be discriminated based on developmental location, using the expression of Corl1 as an indicator.

摘要翻译： 作为筛选通过减法法在胎儿小鼠脑区域中选择性表达的基因的结果，本发明人获得了编码Corl1的cDNA片段。 通过RT-PCR，原位杂交和使用多克隆抗体的免疫染色检测Corl1的表达。 结果表明，Corl1在胚胎期期间在中枢神经系统的高水平选择性表达。 比较使用胚胎脊髓中各种标记物测定的Corl1的表达模式，以鉴定表达Corl1的神经元的类型。 结果表明，Corl1在脊髓中间神经元dI4，dI5，dILA和dILB中特异性表达。 因此，本发明提供了使用Corl1的表达作为指标的dI4和dI6之间的区别，以前仅可以基于发育位置鉴别神经元。

公开(公告)号：US20120252021A1

公开(公告)日：2012-10-04

申请号：US13492733

申请日：2012-06-08

申请人： Yuichi Ono ,  Yoshimasa Sakamoto

发明人： Yuichi Ono ,  Yoshimasa Sakamoto

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： C07K14/705 ,  A61K35/30 ,  A61K38/00 ,  C07K16/28 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5058 ,  G01N33/5073 ,  G01N33/56966 ,  G01N2333/70571 ,  Y10S530/81

摘要： An object of the present invention is to provide a probe, a primer, a primer set and an antibody for use in the detection or selection of a dopaminergic neuron progenitor cell. The present invention provides a probe, a primer and a primer set for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron proliferative progenitor cell, which can hybridize with a nucleotide sequence of a 187A5 gene, or a complementary sequence thereto, and an antibody for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron progenitor cell, which is capable of binding to a 187A5 protein.

摘要翻译： 本发明的目的是提供用于检测或选择多巴胺能神经元祖细胞的探针，引物，引物组和抗体。 本发明提供了用于检测或选择中脑多巴胺能神经元祖细胞的探针，引物和引物组，优选多巴胺能神经元增殖祖细胞，其可以与187A5基因的核苷酸序列杂交，或 其互补序列，以及用于检测或选择能够结合187A5蛋白的中脑多巴胺能神经元祖细胞，优选多巴胺能神经元祖细胞的抗体。

公开(公告)号：US08604173B2

公开(公告)日：2013-12-10

申请号：US13492733

申请日：2012-06-08

申请人： Yuichi Ono ,  Yoshimasa Sakamoto

发明人： Yuichi Ono ,  Yoshimasa Sakamoto

IPC分类号： C12N5/16 ,  C07K16/28 ,  C12Q1/68

CPC分类号： C07K14/705 ,  A61K35/30 ,  A61K38/00 ,  C07K16/28 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5058 ,  G01N33/5073 ,  G01N33/56966 ,  G01N2333/70571 ,  Y10S530/81

摘要： An object of the present invention is to provide a probe, a primer, a primer set and an antibody for use in the detection or selection of a dopaminergic neuron progenitor cell. The present invention provides a probe, a primer and a primer set for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron proliferative progenitor cell, which can hybridize with a nucleotide sequence of a 187A5 gene, or a complementary sequence thereto, and an antibody for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron progenitor cell, which is capable of binding to a 187A5 protein.

摘要翻译： 本发明的目的是提供用于检测或选择多巴胺能神经元祖细胞的探针，引物，引物组和抗体。 本发明提供了用于检测或选择中脑多巴胺能神经元祖细胞的探针，引物和引物组，优选多巴胺能神经元增殖祖细胞，其可以与187A5基因的核苷酸序列杂交，或 其互补序列，以及用于检测或选择能够结合187A5蛋白的中脑多巴胺能神经元祖细胞，优选多巴胺能神经元祖细胞的抗体。

公开(公告)号：US08198081B2

公开(公告)日：2012-06-12

申请号：US12296915

申请日：2007-04-11

申请人： Yuichi Ono ,  Yoshimasa Sakamoto

发明人： Yuichi Ono ,  Yoshimasa Sakamoto

IPC分类号： C07K16/28 ,  C12N5/079

CPC分类号： C07K14/705 ,  A61K35/30 ,  A61K38/00 ,  C07K16/28 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5058 ,  G01N33/5073 ,  G01N33/56966 ,  G01N2333/70571 ,  Y10S530/81

摘要： An object of the present invention is to provide a probe, a primer, a primer set and an antibody for use in the detection or selection of a dopaminergic neuron progenitor cell. The present invention provides a probe, a primer and a primer set for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron proliferative progenitor cell, which can hybridize with a nucleotide sequence of a 187A5 gene, or a complementary sequence thereto, and an antibody for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron progenitor cell, which is capable of binding to a 187A5 protein.

摘要翻译： 本发明的目的是提供用于检测或选择多巴胺能神经元祖细胞的探针，引物，引物组和抗体。 本发明提供了用于检测或选择中脑多巴胺能神经元祖细胞的探针，引物和引物组，优选多巴胺能神经元增殖祖细胞，其可以与187A5基因的核苷酸序列杂交，或 其互补序列，以及用于检测或选择能够结合187A5蛋白的中脑多巴胺能神经元祖细胞，优选多巴胺能神经元祖细胞的抗体。

公开(公告)号：US20140193836A1

公开(公告)日：2014-07-10

申请号：US14234361

申请日：2012-07-27

申请人： Jun Takahashi ,  Daisuke Doi ,  Bumpei Samata ,  Yuichi Ono

发明人： Jun Takahashi ,  Daisuke Doi ,  Bumpei Samata ,  Yuichi Ono

IPC分类号： G01N33/569

CPC分类号： G01N33/56966 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/727 ,  C12N2502/1394 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2509/00

摘要： The present invention provides a method for selecting dopaminergic neuron progenitor cells, which comprises detecting any one or more of markers selected from the group consisting of CD15 (SSEA-1), CD24, CD46, CD47, CD49b, CD57, CD58, CD59, CD81, CD90, CD98, CD147, CD184, Disalogangliosid GD2, SSEA-4, CD49f, SERINC4, CCR9, PHEX, TMPRSS11E, HTR1E, SLC25A2, Ctxn3, Cc17, Chrnb4, Chrna3, Kcnv2, Grm2, Syt2, Lim2, Mboat1, St3ga16, Slc39a12, Tacr1, Lrtm1, Dscam and CD201.

摘要翻译： 本发明提供了选择多巴胺能神经元祖细胞的方法，其包括检测选自CD15（SSEA-1），CD24，CD46，CD47，CD49b，CD57，CD58，CD59，CD81的任何一种或多种标志物 ，CD90，CD98，CD147，CD184，Disalogangliosid GD2，SSEA-4，CD49f，SERINC4，CCR9，PHEX，TMPRSS11E，HTR1E，SLC25A2，Ctxn3，Cc17，Chrnb4，Chrna3，Kcnv2，Grm2，Syt2，Lim2，Mboat1，St3ga16， Slc39a12，Tacr1，Lrtm1，Dscam和CD201。