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公开(公告)号：US08906666B2
公开(公告)日：2014-12-09
申请号：US12994100
申请日：2009-05-22
申请人： Keith Alsaker , Daniel Grenfell-Lee , Michael Hudson , Adam Lawrence , Jessica McGrath , David Young
发明人： Keith Alsaker , Daniel Grenfell-Lee , Michael Hudson , Adam Lawrence , Jessica McGrath , David Young
摘要： The present disclosure provides improved systems for the biological production of aliphatic alcohol compounds. In particular, the present disclosure provides biological systems that show improved resistance to aliphatic alcohol toxicity; in some embodiments, such improved resistance allows for increased levels of aliphatic alcohol production. In one aspect, the present disclosure provides engineered microorganisms that both produce an aliphatic alcohol compound and show resistance to that compound as measured by an ability to grow to predetermined levels in the presence of a given concentration of the compound.
摘要翻译： 本公开提供了用于脂族醇化合物的生物制备的改进的系统。 特别地，本公开提供了显示改善的抗脂肪醇毒性的生物系统; 在一些实施方案中，这种改进的抗性允许增加脂族醇生产水平。 在一个方面，本公开提供了工程化的微生物，其既产生脂族醇化合物，并且在给定浓度的化合物存在下通过生长至预定水平的能力来测量该化合物的抗性。
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22.发明授权公开(公告)号：US08870983B2
公开(公告)日：2014-10-28
申请号：US13162184
申请日：2011-06-16
申请人： James J. Baustian , Leslie R. Wolf
发明人： James J. Baustian , Leslie R. Wolf
CPC分类号： C10L1/1824 , C10L1/023 , C10L1/06 , G01N33/0004
摘要： A gasoline blend and a method for producing a gasoline blend containing low concentrations of a butanol isomer and having good cold start and warm-up driveability characteristics are disclosed.
摘要翻译： 公开了一种汽油混合物和一种生产含有低浓度丁醇异构体并且具有良好的冷起动和预热驱动特性的汽油共混物的方法。
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23.发明申请公开(公告)号：US20140273130A1
公开(公告)日：2014-09-18
申请号：US14213274
申请日：2014-03-14
IPC分类号： C12P7/16
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products. Host cells provided herein comprise a pyruvate-utilizing pathway and a competitive growth advantage over other microorganisms in solution.
摘要翻译： 本文提供重组酵母宿主细胞及其用于生产发酵产物的方法。 本文提供的宿主细胞包含丙酮酸利用途径和相对于溶液中的其它微生物的竞争性生长优势。
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24.发明申请IN SITU EXPRESSION OF LIPASE FOR ENZYMATIC PRODUCTION OF ALCOHOL ESTERS DURING FERMENTATION 审中-公开在发酵期间用于生产酒精饮料的脂肪酶的实际表达公开(公告)号：US20140256020A1
公开(公告)日：2014-09-11
申请号：US14275432
申请日：2014-05-12
CPC分类号： C12N15/81 , A23K10/10 , A23K10/18 , C12N9/20 , C12P7/06 , C12P7/16 , C12P7/62 , C12P7/6436 , Y02E50/10 , Y02E50/17
摘要： Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.
摘要翻译： 本文公开了在发酵期间通过提供产生醇的微生物来生产醇酯的方法，其进一步包含编码具有脂肪酶活性的多肽的工程化多核苷酸。
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公开(公告)号：US20140234929A1
公开(公告)日：2014-08-21
申请号：US14262382
申请日：2014-04-25
CPC分类号： C12P7/06 , C12M21/12 , C12M29/18 , C12M45/04 , C12M45/05 , C12P7/16 , Y02E50/17 , Y10T29/49716
摘要： The present invention relates to processes for recovering butanol produced in a fermentative process using, for example, an ethanol production plant which has been reversibly-retrofitted for butanol production, that is, the ethanol production plant may be converted for butanol production, but can also revert to an ethanol production. The present invention also relates to processes for recovering butanol produced in a fermentative process in a butanol production plant that may be converted to ethanol production plant.
摘要翻译： 本发明涉及使用例如可逆地改性丁醇生产的乙醇生产装置，即发酵过程中生产的丁醇，即乙醇生产装置可以转化为丁醇生产，但也可以 回到乙醇生产。 本发明还涉及在可以转化为乙醇生产设备的丁醇生产设备中的发酵过程中回收生产的丁醇的方法。
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公开(公告)号：US20140106419A1
公开(公告)日：2014-04-17
申请号：US14052184
申请日：2013-10-11
发明人： Stephane Francois Bazzana , Adam Bernfeld , Keith H. Burlew , Duncan Coffey , James Timothy Cronin , Benjamin Fuchs , John W. Hallam , Adam Jones , David J. Lowe , Brian Michael Roesch , Mathias E. Stolarski , James Gregory Wood , Joseph J. Zaher
摘要： The present invention relates to processes and systems for the production of fermentation products such as alcohols. The present invention also provides methods for separating feed stream components for improved biomass processing and productivity.
摘要翻译： 本发明涉及用于生产发酵产物如醇的方法和系统。 本发明还提供了用于分离进料流组分以提高生物质处理和生产率的方法。
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27.发明授权Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans 有权丁醇脱氢酶从细菌无色杆菌xylosoxidans公开(公告)号：US08691540B2
公开(公告)日：2014-04-08
申请号：US13479643
申请日：2012-05-24
IPC分类号： C12P7/16 , C12N9/04 , C12N1/12 , C12N1/13 , C12N1/14 , C12N1/21 , C12N15/56 , C12N1/18 , C12N1/20
摘要： From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
摘要翻译： 从分离自环境样品的细菌菌株中，在含有1-丁醇作为碳源的培养基中富集后，鉴定出具有丁醇脱氢酶活性的新酶。 该酶可将丁醛转化为1-丁醇，异丁醛至异丁醇，以及2-丁酮转化为2-丁醇，因此可用于生产这些底物的重组微生物宿主中丁醇的生物合成。 将编码基因命名为sadB，从鉴定为无色杆菌木糖苷的分离株的菌株中分离出来。
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28.发明申请Method for Producing Butanol using Extractive Fermentation with Electrolyte Addition 有权使用电解质添加法提取发酵生产丁醇的方法公开(公告)号：US20140080189A1
公开(公告)日：2014-03-20
申请号：US14085157
申请日：2013-11-20
IPC分类号： C12P7/16
摘要： A method for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible organic extractant in the presence of at least one electrolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the salt concentration of the basal fermentation medium, is provided. The electrolyte may comprise a salt which dissociates in the fermentation medium, or in the aqueous phase of a biphasic fermentation medium, to form free ions. Also provided is a method and composition for recovering butanol from a fermentation medium.
摘要翻译： 一种通过微生物发酵生产丁醇的方法，其中在发酵过程中将丁醇产物在至少一种电解质存在下，以至少足以提高丁醇分配系数的浓度至少提高至不溶于水的有机萃取剂中去除 在提供基础发酵培养基的盐浓度的情况下。 电解质可以包含在发酵培养基中或在双相发酵培养基的水相中解离形成游离离子的盐。 还提供了用于从发酵培养基中回收丁醇的方法和组合物。
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29.发明申请公开(公告)号：US20140024094A1
公开(公告)日：2014-01-23
申请号：US13889999
申请日：2013-05-08
CPC分类号： C12P7/16 , C12N9/1025 , C12N9/1029 , C12N9/1096 , C12N9/88 , C12N15/81 , C12Y203/03013 , C12Y206/01042 , C12Y401/01001 , C12Y403/01019 , Y02E50/10 , Y02P20/52
摘要： Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase.
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公开(公告)号：US08617861B2
公开(公告)日：2013-12-31
申请号：US12952480
申请日：2010-11-23
摘要： A method for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible organic extractant in the presence of at least one electrolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the salt concentration of the basal fermentation medium, is provided. The electrolyte may comprise a salt which dissociates in the fermentation medium, or in the aqueous phase of a biphasic fermentation medium, to form free ions. Also provided is a method and composition for recovering butanol from a fermentation medium.
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