公开(公告)号：US20100181288A1

公开(公告)日：2010-07-22

申请号：US12691407

申请日：2010-01-21

Applicant: Cha-Mei Tang ,  Olga V. Makarova ,  Mark A. Hoffbauer ,  Todd L. Williamson ,  Platte T. Amstutz

Inventor： Cha-Mei Tang ,  Olga V. Makarova ,  Mark A. Hoffbauer ,  Todd L. Williamson ,  Platte T. Amstutz

IPC: B44C1/22

CPC classification number: B01D67/0062 ,  B01D67/0034 ,  B01D67/0093 ,  B01D69/02 ,  B01D2323/38 ,  B01D2325/028 ,  B01D2325/26

Abstract: Micro- and nanofilters have a wide range of applications in many fields, including medical diagnostics, drug delivery, medical implants, and hemodialysis. Some issues that limit commercial application of current nanofilters in medicine are low pore density, non-uniform pore size, and the use of materials that are not biocompatible. A method is described to fabricate high porosity polymer and diamond micro- and nanofilters producing smooth, uniform and straight pores of high aspect ratio. Pore size, density, and shape can be predetermined with a high degree of precision by masks and controlled etch. The method combines energetic neutral atom beam lithography and a mask. This technology allows etching polymeric materials in a clean, well-controlled, and charge-free environment, making it very suitable for fabricating nanofilters and other components for biomedical applications.

Abstract translation: 微型和纳米过滤器在许多领域有广泛的应用，包括医疗诊断，药物输送，医疗植入物和血液透析。 限制当前纳米过滤器在医药中的商业应用的一些问题是低孔密度，不均匀孔径以及不能生物相容的材料的使用。 描述了一种制造高孔隙率聚合物和金刚石微米和纳米过滤器的方法，其产生高纵横比的光滑，均匀和直孔。 孔径，密度和形状可以通过掩模和受控蚀刻以高精确度预先确定。 该方法结合了高能中性原子束光刻和掩模。 该技术允许在干净，良好控制和无电荷环境中蚀刻聚合物材料，使其非常适合制造用于生物医学应用的纳米过滤器和其他部件。

公开(公告)号：US20080088059A1

公开(公告)日：2008-04-17

申请号：US11984634

申请日：2007-11-20

Applicant: Cha-Mei Tang ,  Olga Makarova ,  Platte Amstutz ,  Guohua Yang

Inventor： Cha-Mei Tang ,  Olga Makarova ,  Platte Amstutz ,  Guohua Yang

IPC: B29C39/10 ,  B29C69/00 ,  B29C70/84

CPC classification number: G21K1/025

Abstract: Grids and collimators, for use with electromagnetic energy emitting devices, include at least a metal layer that is formed, for example, by electroplating/electroforming or casting. The metal layer includes top and bottom surfaces, and a plurality of solid integrated walls. Each of the solid integrated walls extends from the top to bottom surface and has a plurality of side surfaces. The side surfaces of the solid integrated walls are arranged to define a plurality of openings extending entirely through the layer. At least some of the walls also can include projections extending into the respective openings formed by the walls. The projections can be of various shapes and sizes, and are arranged so that a total amount of wall material intersected by a line propagating in a direction along an edge of the grid is substantially the same as another total amount of wall material intersected by another line propagating in another direction substantially parallel to the edge of the grid at any distance from the edge. Methods to fabricate these grids using copper, lead, nickel, gold, any other electroplating/electroforming materials, metal composites or low melting temperature metals are described.

Abstract translation: 用于电磁能发射装置的栅格和准直器至少包括通过例如电镀/电铸或铸造形成的金属层。 金属层包括顶表面和底表面，以及多个固体整​​体壁。 每个固体一体化壁从顶部到底部表面延伸并且具有多个侧表面。 固体一体化壁的侧表面布置成限定完全延伸穿过该层的多个开口。 至少一些壁还可以包括延伸到由壁形成的相应开口中的突起。 突起可以是各种形状和尺寸，并且被布置成使得沿着沿着栅格的边缘的方向传播的线相交的壁材的总量基本上与另一个与另一条线相交的壁材料的总量相同 在距离边缘任何距离处基本上平行于栅格的边缘的另一方向上传播。 描述了使用铜，铅，镍，金，任何其它电镀/电铸材料，金属复合材料或低熔点金属制造这些栅极的方法。

公开(公告)号：US07197193B2

公开(公告)日：2007-03-27

申请号：US10429258

申请日：2003-05-05

Applicant: Shuhong Li ,  Cha-Min Tang ,  Cha-Mei Tang

Inventor： Shuhong Li ,  Cha-Min Tang ,  Cha-Mei Tang

IPC: G06K9/36

CPC classification number: G06T7/55 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: An instrument to acquire and methods to obtain three-dimensional (3D) images from a series of two-dimensional (2D) images which are obtained without moving the relative positions of the target, the detector, or the focusing lens is disclosed. The 2D images consist of one centered image obtained with the aperture at the center of optical system, and at least two directional images obtained with apertures at off-axis locations. The images can be obtained simultaneously or sequentially. The blurred 2D images are sectioned by computational method using point spread function of the optical system resulting in a set of decoupled 2D layers of the 3D object. The layered images are then sharpened by deconvolution using point spread function. The 3D reconstructed image is displayed. This technique provides fast data acquisition and fast image reconstruction and eliminates problems associated with motion, phototoxicity and photobleaching.

Abstract translation: 公开了一种用于获取并从一系列二维（2D）图像中获得三维（3D）图像的仪器，该方法在不移动目标，检测器或聚焦透镜的相对位置的情况下获得。 2D图像由在光学系统的中心处的孔径获得的一个中心图像和在离轴位置处具有孔径获得的至少两个定向图像组成。 图像可以同时或顺序获得。 通过使用光学系统的点扩散函数的计算方法对模糊的2D图像进行分割，得到3D对象的一组去耦2D层。 然后通过使用点扩散函数的去卷积来锐化分层图像。 显示3D重建图像。 该技术提供快速数据采集和快速图像重建，并消除与运动，光毒性和光漂白相关的问题。

公开(公告)号：US20030228053A1

公开(公告)日：2003-12-11

申请号：US10429258

申请日：2003-05-05

Applicant: Creatv MicroTech, Inc.

Inventor： Shuhong Li ,  Cha-Min Tang ,  Cha-Mei Tang

IPC: G06T007/00

CPC classification number: G06T7/55 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: An instrument to acquire and methods to obtain three-dimensional (3D) images from a series of two-dimensional (2D) images which are obtained without moving the relative positions of the target, the detector, or the focusing lens is disclosed. The 2D images consist of one centered image obtained with the aperture at the center of optical system, and at least two directional images obtained with apertures at off-axis locations. The images can be obtained simultaneously or sequentially. The blurred 2D images are sectioned by computational method using point spread function of the optical system resulting in a set of decoupled 2D layers of the 3D object. The layered images are then sharpened by deconvolution using point spread function. The 3D reconstructed image is displayed. This technique provides fast data acquisition and fast image reconstruction and eliminates problems associated with motion, phototoxicity and photobleaching.

Abstract translation: 公开了一种用于获取并从一系列二维（2D）图像中获得三维（3D）图像的仪器，该图像在不移动目标，检测器或聚焦透镜的相对位置的情况下获得。 2D图像由在光学系统的中心处的孔径获得的一个中心图像和在离轴位置处具有孔径获得的至少两个定向图像组成。 图像可以同时或顺序获得。 通过使用光学系统的点扩散函数的计算方法对模糊的2D图像进行分割，得到3D对象的一组去耦2D层。 然后通过使用点扩散函数的去卷积来锐化分层图像。 显示3D重建图像。 该技术提供快速数据采集和快速图像重建，并消除与运动，光毒性和光漂白相关的问题。

公开(公告)号：US5949850A

公开(公告)日：1999-09-07

申请号：US879258

申请日：1997-06-19

Applicant: Cha-Mei Tang

Inventor： Cha-Mei Tang

IPC: G21K1/02 ,  G21K1/00

CPC classification number: G03F7/0007 ,  G03F7/201 ,  G21K1/025

Abstract: Methods for making large area grids consisting of focused and unfocused holes in sheets of metal. The grid consists of thin metal walls surrounding hollow openings. The projection of all walls converge to a focal spot in the focused grid, and on parallel or substantially parallel in an unfocused grid. A grid having a large area is made by interlocking together smaller grid pieces. A tall device is made by stacking layers of focused grids. Methods to make unfocused grids that detect parallel incoming x-rays are also described. The opening of the grid can be filled with phosphor or other scintillating materials to make a integrated grid/scintillator structure. These grids can also be applied to many applications such as x-ray antiscatter for diagnostic medical imaging, non-destructive testing, x-ray collimation, filters for liquids or electromagnetic radiation.

Abstract translation: 制造大面积网格的方法，由金属片中的聚焦和未聚焦的孔组成。 网格由围绕中空开口的薄金属壁组成。 所有墙壁的投影集中在聚焦网格中的焦点，并且在未聚焦网格中平行或基本平行。 具有大面积的网格通过将较小的网格片互锁在一起而制成。 高度的装置是通过堆叠聚焦的网格层来制造的。 还描述了使未聚焦网格检测平行进入的X射线的方法。 网格的开口可以填充磷光体或其他闪烁材料，以形成集成的栅格/闪烁体结构。 这些网格也可以应用于许多应用，例如用于诊断医学成像，非破坏性测试，x射线准直，液体或电磁辐射过滤器的x射线反散射。

公开(公告)号：US20240312665A1

公开(公告)日：2024-09-19

申请号：US18377523

申请日：2023-10-06

Applicant: Creatv MicroTech, Inc.

Inventor： Olga V. Makarova ,  Ralu Divan ,  Nicolaie Moldovan ,  David A. Czaplewski ,  Cha-Mei Tang

IPC: G21K1/10 ,  G21K7/00

CPC classification number: G21K1/10 ,  G21K7/00

Abstract: High-resolution, X-ray phase contrast microscopy, a key technique with promising potential in biomedical imaging and diagnostics, is based on narrow-slit high-aspect-ratio gold gratings. We present the development, fabrication details, and experimental testing of the freestanding 10-μm-thick gold membrane masks with an array of 0.9-1.5 □m void slit apertures for a novel low-energy X-ray microscope. The overall mask size is 4 mm×4 mm, with a grating pitch of 7.5 □m, 6.0-6.6-□m-wide gold bars are supported by 3-□m-wide crosslinks at 400 □m intervals. The fabrication process is based on gold electroplating into a silicon mold coated with various thin films to form a voltage barrier, plating base, and sacrificial layer, followed by the mold removal to obtain the freestanding gold membrane with void slit apertures. We discuss key aspects for the materials and processes, including gold structures homogeneity, residual stresses, and prevention of collapsing of the grid elements. We further demonstrate the possibility to obtain high-resolution, high contrast 2D images of biological samples using an incoherent, rotating anode X-ray tube.

公开(公告)号：US20220128544A1

公开(公告)日：2022-04-28

申请号：US17430457

申请日：2020-02-13

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel L. ADAMS ,  Cha-Mei TANG

IPC: G01N33/50 ,  G01N33/574

Abstract: Means for predicting overall survival (OS) and progression free survival (PFS) of subjects having cancer are disclosed, where the predictions are based on the number of CCR5 pools in circulating cells, such as circulating cancer associated macrophage-like cells (CAMLs) and circulating tumor cells (CTCs) found in a biological sample, such as blood, from the subject. CCR5 expression can also be used for companion or complementary diagnostics.

公开(公告)号：US20220034888A1

公开(公告)日：2022-02-03

申请号：US17503872

申请日：2021-10-18

Applicant: CREATV MICROTECH INC.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574 ,  G01N33/50

Abstract: Circulating tumor cells (CTCs) are associated with metastasis of malignant solid tumors in a patient. Presented here is evidence that CTCs exhibit cell cycle phase variability and that there is a strong correlation between the number of CTCs in a mitotic cell cycle phase and the prospects for long term survival of the subject from which the cells were obtained. Also presented herein are methods of determining the mitotic cell cycle phase of CTCs from a patient having cancer and using the information in grading malignant solid tumors and predicting the likelihood of survival of the patient.

公开(公告)号：US11175279B2

公开(公告)日：2021-11-16

申请号：US16454937

申请日：2019-06-27

Applicant: Creatv MicroTech, Inc.

Inventor： Olga Makarova ,  Cha-Mei Tang ,  Peixuan Zhu ,  Shuhong Li ,  Daniel Adams ,  Platte T. Amstutz

IPC: G01N33/50 ,  B01D71/46 ,  G01N1/40 ,  B01L3/00 ,  B01D39/16 ,  B01D29/00 ,  B01L9/00

Abstract: A microfilter having a hydrophilic surface and suited for size-based capture and analysis of cells, such as circulating cancer cells, from whole blood and other human fluids is disclosed. The filter material is photo-definable, allowing the formation of precision pores by UV lithography. Exemplary embodiments provide a device that combines a microfilter with 3D nanotopography in culture scaffolds that mimic the 3D in vivo environment to better facilitate growth of captured cells.

公开(公告)号：US11156596B2

公开(公告)日：2021-10-26

申请号：US16369730

申请日：2019-03-29

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel Adams ,  Cha-Mei Tang

IPC: G01N33/49 ,  G01N33/574 ,  G01N33/487 ,  G01N33/569

Abstract: A new sensitive cell biomarker of solid tumors is identified in blood. This biomarker can be used to determine presence of solid tumors, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.