公开(公告)号：US20120164698A1

公开(公告)日：2012-06-28

申请号：US13379654

申请日：2010-06-18

申请人： Volker F. Wendisch ,  Steffen Lindner ,  Brigitte Bathe ,  Wilfried Claes

发明人： Volker F. Wendisch ,  Steffen Lindner ,  Brigitte Bathe ,  Wilfried Claes

IPC分类号： C12P13/04 ,  C12P13/12 ,  C12N1/21 ,  C12P13/22 ,  C12N15/54 ,  C12P13/08 ,  C12P13/06

CPC分类号： C12P13/08 ,  C12N9/1205 ,  C12Y207/01023

摘要： The invention relates to a method for preparing organic-chemical compounds, characterized in that the following steps are carried out: a) fermentation of a microorganism secreting an L-amino acid, which microorganism contains an overexpressed polynucleotide coding for a polypeptide having polyphosphate-dependent NAD+ kinase activity, in a fermentation medium, to form a fermentation broth, b) accumulation of said compound in said fermentation broth and/or in the cells of said microorganism. The invention relates to a method for preparing organic-chemical compounds by fermentation of a microorganism in which a polypeptide having polyphosphate-dependent NAD+ kinase is overexpressed.

摘要翻译： 本发明涉及一种制备有机化合物的方法，其特征在于进行以下步骤：a）分泌L-氨基酸的微生物的发酵，该微生物含有编码具有多磷酸依赖性多肽的过表达多核苷酸 NAD +激酶活性，在发酵培养基中形成发酵液，b）所述化合物在所述发酵液中和/或所述微生物细胞中的积累。 本发明涉及通过发酵其中具有多磷酸依赖性NAD +激酶的多肽被过表达的微生物来制备有机化合物的方法。

公开(公告)号：US08202705B2

公开(公告)日：2012-06-19

申请号：US12841909

申请日：2010-07-22

申请人： Masaki Saito

发明人： Masaki Saito

IPC分类号： C12P19/26 ,  C12N15/54 ,  C12N9/10 ,  C12N5/10

CPC分类号： C12N9/1081

摘要： A sialyltransferase having the following physico-chemical properties:(1) Activity: transfers sialic acid from a sialic acid donor selectively to a 3-hydroxyl group of a galactose residue contained in lactosylceramide as a sialic acid acceptor to produce ganglioside GM3; (2) Optimal Reaction pH: 6.0 to 7.0; and (3) Inhibition and Activation: the activity increases at least 1.5 times with 10 mM of Mn2+ as compared with the case in the absence thereof.

摘要翻译： 具有以下物理化学性质的唾液酸转移酶：（1）活性：将唾液酸供体的唾液酸选择性转移到作为唾液酸受体的乳糖神经酰胺中含有的半乳糖残基的3-羟基以产生神经节苷脂GM3; （2）最佳反应pH：6.0〜7.0; 和（3）抑制和活化：与没有Mn 2+的情况相比，活性增加至少1.5倍。

公开(公告)号：US20120144523A1

公开(公告)日：2012-06-07

申请号：US13389815

申请日：2010-08-04

申请人： Jonathan E. Page ,  Zakia Boubakir

发明人： Jonathan E. Page ,  Zakia Boubakir

IPC分类号： C12N15/82 ,  C12N9/10 ,  C12N5/04 ,  C12P1/00 ,  C12N15/70 ,  C12N15/81 ,  C12N15/54 ,  C12N15/63

CPC分类号： C12N9/1085 ,  C12N15/8243 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12Y205/01

摘要： Nucleic acid molecules from Cannabis sativa (cannabis, hemp, marijuana) have been isolated and characterized, and encode polypeptides having aromatic prenyltransferase activity. Specifically, the enzyme, CsPT1, is a geranylpyrophosphate olivetolate geranyltransferase, active in the cannabinoid biosynthesis step of prenylation of olivetolic acid to form cannabigerolic acid (CBGA). Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.

摘要翻译： 已经分离并表征了大麻（大麻，大麻，大麻）的核酸分子，并编码具有芳族异戊烯基转移酶活性的多肽。 具体地说，酶CsPT1是一种杀稗草酰焦磷酸酯橄榄油醇甘油转移酶，在大肠杆菌生物合成步骤中活化橄榄油酸，以形成大麻酚酸（CBGA）。 核酸的表达或过表达改变大麻素化合物的水平。 多肽可以在体内或体外用于产生大麻素化合物。

公开(公告)号：US08168417B2

公开(公告)日：2012-05-01

申请号：US12972306

申请日：2010-12-17

申请人： Randy Berka ,  Michael Rey ,  Preethi Ramaiya ,  Jens Tønne Andersen ,  Michael Dolberg Rasmussen ,  Peter Bjarke Olsen

发明人： Randy Berka ,  Michael Rey ,  Preethi Ramaiya ,  Jens Tønne Andersen ,  Michael Dolberg Rasmussen ,  Peter Bjarke Olsen

IPC分类号： C12N9/10 ,  C12N15/54

CPC分类号： C12Q1/689 ,  C07K14/32

摘要： The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.

公开(公告)号：US20120100620A1

公开(公告)日：2012-04-26

申请号：US13272609

申请日：2011-10-13

申请人： Juergen Nett

发明人： Juergen Nett

IPC分类号： C12N15/81 ,  C12N15/54 ,  C12N1/19

CPC分类号： C12N15/52 ,  C12N15/815 ,  C12P13/08

摘要： Disclosed are the LYS1, LYS2, LYS4, LYS5, and LYS9 genes encoding various enzymes in the lysine biosynthesis pathway of Pichia pastoris. The loci in the Pichia pastoris genome encoding these enzymes are useful sites for stable integration of heterologous nucleic acid molecules into the Pichia pastoris genome. The genes or gene fragments encoding the particular enzymes may be used as selection markers for constructing recombinant Pichia pastoris.

摘要翻译： 公开了在毕赤酵母的赖氨酸生物合成途径中编码各种酶的LYS1，LYS2，LYS4，LYS5和LYS9基因。 编码这些酶的巴斯德毕赤酵母基因组中的基因座是将异源核酸分子稳定整合到巴斯德毕赤酵母基因组中的有用位点。 可以将编码特定酶的基因或基因片段用作构建重组巴斯德毕赤酵母的选择标记。

公开(公告)号：US20120096593A1

公开(公告)日：2012-04-19

申请号：US13318995

申请日：2010-04-28

申请人： Yves Hatzfeld ,  Ana Isabel Sanz Molinero ,  Valerie Frankard ,  Christophe Reuzeau

发明人： Yves Hatzfeld ,  Ana Isabel Sanz Molinero ,  Valerie Frankard ,  Christophe Reuzeau

IPC分类号： A01H1/06 ,  A01H5/00 ,  A01H5/10 ,  C07K14/415 ,  A01H5/06 ,  C12N15/54 ,  C12N15/29 ,  C12N9/12 ,  C12N15/82 ,  C12N5/10

CPC分类号： C12N15/82 ,  C07K14/415

摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a LDOX (leucoanthocyanidin dioxygenase) polypeptide, a nucleic acid encoding a YRP5, a nucleic acid encoding a CK1 (Casein Kinase type I) polypeptide, a nucleic acid encoding a bHLH12-like (basic Helix Loop Helix group polypeptide, a nucleic acid encoding an ADH2 polypeptide or a nucleic acid encoding a GCN5-like polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an LDOX polypeptide, or a YRP5 polypeptide, or a CK1 polypeptide, or a bHLH12-like polypeptide, or an ADH2 polypeptide, or a GCN5-like polypeptide, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. The invention also provides hitherto unknown CK1-encoding nucleic acids and hitherto unknown bHLH12-like-encoding nucleic acids useful in per-forming the methods of the invention.

摘要翻译： 本发明一般涉及分子生物学领域，涉及通过调节植物中编码LDOX（无色花青素双加氧酶）多肽的核酸，编码YRP5的核酸，核酸的核酸表达来改善各种植物生长特性的方法 编码CK1（酪蛋白激酶I）多肽，编码bHLH12样的核酸（碱性螺旋环螺旋组多肽，编码ADH2多肽的核酸或编码GCN5样多肽的核酸），本发明还涉及 具有调节编码LDOX多肽或YRP5多肽或CK1多肽或bHLH12样多肽或ADH2多肽或GCN5样多肽的核酸的表达的植物，其相对于 相应的野生型植物或其他对照植物，本发明还提供了可用于本发明方法的构建体 提供迄今为止未知的CK1编码核酸和迄今未知的bHLH12样编码核酸，其可用于形成本发明的方法。

公开(公告)号：US20120096588A1

公开(公告)日：2012-04-19

申请号：US13328677

申请日：2011-12-16

申请人： Knut Meyer ,  Howard Glenn Damude ,  Kevin G. Ripp ,  Kevin L. Stecca

发明人： Knut Meyer ,  Howard Glenn Damude ,  Kevin G. Ripp ,  Kevin L. Stecca

IPC分类号： A01H5/00 ,  C12N9/10 ,  C12N15/80 ,  C12N5/10 ,  A01H5/10 ,  C12N1/19 ,  C12N15/54 ,  C12N15/82

CPC分类号： C12N15/8247 ,  C12N9/1029 ,  C12Y203/0102

摘要： Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.

摘要翻译： 描述了与非转基因，无偏分离大豆种子的总脂肪酸含量相比，具有至少10％的总脂肪酸含量增加和改变的脂肪酸分布的转基因大豆种子。 来自含油生物的DGAT基因用于实现种子储存脂质的增加。

公开(公告)号：US20120094332A1

公开(公告)日：2012-04-19

申请号：US12982804

申请日：2010-12-30

申请人： Jun Lee ,  Gary Gerard ,  Harini Shandilya ,  Katherine R. Griffiths ,  Moreland D. Gibbs ,  Peter L. Bergquist ,  Robert Jason Potter

发明人： Jun Lee ,  Gary Gerard ,  Harini Shandilya ,  Katherine R. Griffiths ,  Moreland D. Gibbs ,  Peter L. Bergquist ,  Robert Jason Potter

IPC分类号： C12P19/34 ,  C12N9/12 ,  C12N15/54

CPC分类号： C12N9/1252 ,  C12N9/1276 ,  C12N9/14 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Y207/07007 ,  C12Y306/01 ,  C12Q2521/101

摘要： The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.

摘要翻译： 本发明提供具有核苷酸聚合酶活性的多肽和增强聚合酶活性的方法。 本发明的多肽可以具有DNA依赖性DNA聚合酶活性和RNA依赖性DNA聚合酶活性，即逆转录酶活性。 本发明的多肽可用于任何应用，包括但不限于DNA测序反应，扩增反应，cDNA合成反应以及合并的cDNA合成和扩增反应，例如RT-PCR。

公开(公告)号：US08148124B2

公开(公告)日：2012-04-03

申请号：US11736504

申请日：2007-04-17

申请人： Michael R. Stallcup ,  Dagang Chen ,  Heng Hong ,  Dana W. Aswad

发明人： Michael R. Stallcup ,  Dagang Chen ,  Heng Hong ,  Dana W. Aswad

IPC分类号： C12N9/10 ,  C12N15/54

CPC分类号： C07K16/40 ,  C12N9/1003

摘要： The invention relates to the cDNA and deduced amino acid sequence of the Coactivator Associated arginine (R) Methyltransferase protein, CARM1. A method is described for the use CARM1 to regulate gene expression in vivo. CARM1 has also been used to methylate arginine residues of histones, synthetic peptides, and other proteins. A method to use CARM1 to screen for drugs that inhibit its methyltransferase activity is also described, as is a method to screen for drugs that modulate CARM1's interactions with other proteins.

公开(公告)号：US20120070863A1

公开(公告)日：2012-03-22

申请号：US13218267

申请日：2011-08-25

申请人： Takeshi YAMAMOTO ,  Hiroshi TSUKAMOTO ,  Yoshimitsu TAKAKURA

发明人： Takeshi YAMAMOTO ,  Hiroshi TSUKAMOTO ,  Yoshimitsu TAKAKURA

IPC分类号： C12P19/18 ,  C12N15/54 ,  C12N15/63 ,  C12N1/15 ,  C12N1/21 ,  C12N1/19 ,  C12N5/10 ,  C12N9/10 ,  C12N1/20

CPC分类号： C12P19/26 ,  C12N9/1081 ,  C12Y204/99004

摘要： The present invention provides a novel β-galactoside-α2,3-sialyltransferase and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase, as well as a method for producing the sialyltransferase using such a microorganism. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,3-sialyltransferase. The present invention further provides a method for preparing a sialylsugar chain, which uses the sialyltransferase of the present invention.

摘要翻译： 本发明提供了一种新型的半乳糖苷-α2,3-唾液酸转移酶和编码唾液酸转移酶的核酸。 本发明还提供了产生唾液酸转移酶的微生物，以及使用这种微生物生产唾液酸转移酶的方法。 本发明还提供了携带编码唾液酸转移酶的核酸和载体转化的宿主细胞的载体以及重组α-半乳糖苷-α2,3-唾液酸转移酶的制备方法。 本发明还提供了使用本发明的唾液酸转移酶制备唾液酸糖链的方法。