公开(公告)号：US08039817B2

公开(公告)日：2011-10-18

申请号：US12434495

申请日：2009-05-01

申请人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： G01N21/64

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：US20100187115A1

公开(公告)日：2010-07-29

申请号：US12626353

申请日：2009-11-25

申请人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

发明人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

IPC分类号： B01D57/02 ,  F04B19/00 ,  B01D61/46 ,  B81B7/02 ,  C12Q1/68 ,  B01J19/00 ,  G01N27/447

CPC分类号： F04B37/10 ,  F04B19/00 ,  Y10T436/2575

摘要： An electroosmotic (EO) pump is provided that includes a housing having a pump cavity, a porous core medium and electrodes. The porous core medium is positioned within the pump cavity to form an exterior reservoir that extends at least partially about an exterior surface of the porous core medium. The porous core medium has an open inner chamber provided therein. The inner chamber represents an interior reservoir. The electrodes are positioned in the inner chamber and are positioned proximate the exterior surface. The electrodes induce flow of a fluid through the porous core medium between the interior and exterior reservoirs, wherein a gas is generated when the electrodes induce flow of the fluid. The housing has a fluid inlet to convey the fluid to one of the interior reservoir and the exterior reservoir. The housing has a fluid outlet to discharge the fluid from another of the interior reservoir and the exterior reservoir. The housing has a gas removal device to remove the gas from the pump cavity.

摘要翻译： 提供一种电渗（EO）泵，其包括具有泵腔的壳体，多孔芯介质和电极。 多孔芯介质定位在泵腔内以形成至少部分地围绕多孔芯介质的外表面延伸的外部储存器。 多孔芯介质具有设置在其中的开放内室。 内室表示内部储存器。 电极定位在内室中并且位于外表面附近。 电极通过多孔芯介质在内部和外部储存器之间引导流体流动，其中当电极引起流体流动时产生气体。 壳体具有用于将流体输送到内部储存器和外部储存器中的一个的流体入口。 壳体具有流体出口，以从另一个内部储存器和外部储存器排出流体。 壳体具有用于从泵腔去除气体的气体去除装置。

公开(公告)号：US20100087325A1

公开(公告)日：2010-04-08

申请号：US12565606

申请日：2009-09-23

申请人： Dale Buermann

发明人： Dale Buermann

IPC分类号： C40B30/00 ,  C40B60/12

CPC分类号： C40B60/12 ,  B01L3/502715 ,  B01L7/00 ,  B01L7/525 ,  B01L2300/021 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/168 ,  B01L2300/1822 ,  B01L2300/185

摘要： The present invention provides a novel approach for controlling the temperature of biological samples on a support structure. The support structure may, for instance, be a flow cell through which a reagent fluid is allowed to flow and interact with biological samples. A thermoelectric heat exchange device, such as a Peltier device, may be used to heat or cool the biological samples on the support structure. In addition, a fluid circulating heat exchange device, such as a water heating or cooling system, may be used to heat or cool the thermoelectric heat exchange device. In general, the support structure may be located on top of the thermoelectric heat exchange device which, in turn, may be located on top of the fluid circulating heat exchange device. The thermoelectric heat exchange device and fluid circulating heat exchange device may be integrated into a holder bench which may be part of a station within an imaging processing system. The holder bench may be configured to hold multiple support structures at a time. In addition, the support structures may be configured to be evaluated and imaged using both epifluorescent and total internal reflection (TIRF) excitation techniques.

摘要翻译： 本发明提供了一种用于控制支撑结构上的生物样品的温度的新方法。 支撑结构例如可以是允许试剂流体流过并与生物样品相互作用的流动池。 可以使用诸如珀耳帖装置的热电换热装置来加热或冷却支撑结构上的生物样品。 此外，可以使用诸如水加热或冷却系统的流体循环热交换装置来加热或冷却热电热交换装置。 通常，支撑结构可以位于热电换热装置的顶部，热电换热装置又可以位于流体循环热交换装置的顶部。 热电热交换装置和流体循环热交换装置可以集成到可以是成像处理系统内的站的一部分的保持台中。 支架台可以被配置为一次保持多个支撑结构。 此外，支撑结构可以被配置为使用荧光和全内反射（TIRF）激发技术进行评估和成像。

公开(公告)号：US20050133700A1

公开(公告)日：2005-06-23

申请号：US10745371

申请日：2003-12-22

申请人： Dale Buermann ,  Michael Mandella ,  Stewart Carl ,  Guanghua Zhang ,  Hector Gonzalez-Banos

发明人： Dale Buermann ,  Michael Mandella ,  Stewart Carl ,  Guanghua Zhang ,  Hector Gonzalez-Banos

IPC分类号： G06F3/03 ,  G06F3/033 ,  G06M7/00

CPC分类号： G06F3/03545 ,  G06F3/0321

摘要： A method and apparatus for determining a pose of an elongate object and an absolute position of its tip while the tip is in contact with a plane surface having invariant features. The surface and features are illuminated with a probe radiation and a scattered portion, e.g., the back-scattered portion, of the probe radiation returning from the plane surface and the feature to the elongate object at an angle τ with respect to an axis of the object is detected. The pose is derived from a response of the scattered portion to the surface and the features and the absolute position of the tip on the surface is obtained from the pose and knowledge about the feature. The probe radiation can be directed from the object to the surface at an angle σ to the axis of the object in the form of a scan beam. The scan beam can be made to follow a scan pattern with the aid of a scanning arrangement with one or more arms and one or more uniaxial or biaxial scanners. Angle τ can also be varied, e.g., with the aid of a separate or the same scanning arrangement as used to direct probe radiation to the surface. The object can be a pointer, a robotic arm, a cane or a jotting implement such as a pen, and the features can be edges, micro-structure or macro-structure belonging to, deposited on or attached to the surface which the tip of the object is contacting.

摘要翻译： 一种用于在尖端与具有不变特征的平面接触的情况下，确定细长物体的姿态和其尖端的绝对位置的方法和装置。 表面和特征被探针辐射照射，并且探针辐射的散射部分（例如，背散射部分）从平面表面和特征向细长物体返回，其角度τ相对于 检测到物体。 姿势是从分散部分到表面的响应导出的，并且从关于特征的姿势和知识中获得尖端在表面上的特征和绝对位置。 探针辐射可以以扫描光束的形式从物体以角度与物体的轴线指向表面。 借助于具有一个或多个臂和一个或多个单轴或双轴扫描器的扫描装置，可以使扫描光束跟随扫描图案。 角度tau也可以变化，例如借助于将探针辐射引导到表面的单独或相同的扫描布置。 该物体可以是指针，机器人手臂，手杖或笔记本记录工具，并且特征可以是属于，沉积在或附着到表面上的边缘，微结构或宏观结构，其表面的尖端 对象正在联系。

公开(公告)号：US20050107979A1

公开(公告)日：2005-05-19

申请号：US10701817

申请日：2003-11-04

申请人： Dale Buermann ,  Hector Gonzalez-Banos ,  Michael Mandella ,  Stewart Carl

发明人： Dale Buermann ,  Hector Gonzalez-Banos ,  Michael Mandella ,  Stewart Carl

IPC分类号： G06F3/033 ,  G06F15/00

CPC分类号： G06F3/03545

摘要： An apparatus and method for determining an inclination angle θ between an axis of an elongate object such as a cane, a pointer or a jotting implement such as a pen, pencil, stylus or the like and a normal to a plane surface at times when a tip of the elongate object is contacting that plane surface. The apparatus has an emitter mounted on the object for illuminating the plane surface with a probe radiation at an angle σ with respect to the axis of the object. The apparatus also has a detector mounted on the elongate object for detecting a radiation characteristic of a scattered portion of the probe radiation returning from the plane surface and a computing unit for deriving the inclination angle θ from the radiation characteristic. A scanning arrangement, such as a uniaxial or biaxial scanner, or a light guiding optic can be used for varying angle σ, and the probe radiation can be emitted in the form of a scan beam. Preferably, the emitter and detector of the scattered portion of the probe radiation are integrated and the scattered portion of the probe radiation whose characteristic is being measured is the back-scattered portion. The radiation characteristic detected by the detector can be the intensity, polarization, time-of-flight or any combination thereof.

摘要翻译： 一种用于确定细长物体的轴线之间的倾斜角度θ的装置和方法，所述细长物体诸如手杖，指针或诸如笔，铅笔，触笔等的记录工具以及在平面表面的法线 细长物体的尖端与该平面接触。 该装置具有安装在物体上的发射器，用于以相对于物体的轴线的角度σ来探测辐射照射平面。 该装置还具有安装在细长物体上的检测器，用于检测从平面表面返回的探针辐射的散射部分的辐射特性，以及用于从辐射特性导出倾斜角度θ的计算单元。 可以使用诸如单轴或双轴扫描仪或导光光学器件的扫描装置来改变角度，并且可以以扫描光束的形式发射探测辐射。 优选地，探测辐射的散射部分的发射极和检测器是一体的，并且其特征被测量的探测辐射的散射部分是反向散射部分。 由检测器检测的辐射特性可以是强度，极化，飞行时间或其任何组合。

公开(公告)号：US06441607B1

公开(公告)日：2002-08-27

申请号：US09728809

申请日：2000-12-01

申请人： Dale Buermann ,  Daniel Tran

发明人： Dale Buermann ,  Daniel Tran

IPC分类号： G01R302

CPC分类号： G01R31/2887

摘要： A system for docking a floating test stage with integrated testing equipment to a predetermined position relative to a terrestrial base is described. The system includes a cylinder for docking the test stage to the predetermined position and a vibration isolation system for isolating the test stage when it is floating over the base. The system also includes a handling unit indexed to the predetermined position for loading and unloading samples on the test stage.

摘要翻译： 描述了一种用于将浮动测试台与集成测试设备相对于相对于陆地基座的预定位置对接的系统。 该系统包括用于将测试台对准到预定位置的气缸和用于当测试台浮在基座上时隔离测试台的隔振系统。 系统还包括索引到预定位置的处理单元，用于在测试台上装载和卸载样品。

公开(公告)号：US6084666A

公开(公告)日：2000-07-04

申请号：US233692

申请日：1999-01-19

申请人： Alexander P. Kindwall ,  Dale Buermann

发明人： Alexander P. Kindwall ,  Dale Buermann

IPC分类号： G01N21/01

CPC分类号： G01N21/01

摘要： An apparatus for rotationally positioning a disk on a stationary stage at a constant and repeatable distance and angle for a device to perform an operation on several positions of the disk surface is disclosed. The disk apparatus has a manipulating member that operates from a central portion of the stationary stage. The member raises the disk, rotates the disk to a new position and sets the disk back down on a planar portion of the same stationary stage. The apparatus uses vacuum to secure the disk to the stage and to the member during manipulation. The member is raised by a spring and lowered by vacuum. The apparatus is easily incorporated into an optical system for performing reflectance and transmitance on both side of the disk simultaneously allowing for the analyses of the disk layers for composition and consistency.

摘要翻译： 公开了一种用于以恒定且可重复的距离和角度将盘定位在固定台上的装置，用于使设备在盘表面的多个位置上执行操作。 盘装置具有从固定台的中心部分操作的操作构件。 该成员升起磁盘，将磁盘旋转到一个新位置，并将磁盘设置在相同固定平台的平面部分上。 该装置在操作期间使用真空将盘固定到台架和构件上。 该构件由弹簧升起并由真空降低。 该装置容易地结合到光学系统中，用于在盘的两侧执行反射和透射，同时允许分析磁盘层的组成和一致性。

公开(公告)号：US5880831A

公开(公告)日：1999-03-09

申请号：US987907

申请日：1997-12-09

申请人： Dale Buermann ,  Abdul Rahim Forouhi ,  Michael J. Mandella

发明人： Dale Buermann ,  Abdul Rahim Forouhi ,  Michael J. Mandella

IPC分类号： G01B11/06 ,  G01J3/02 ,  G01J3/12 ,  G01J3/42 ,  G01J4/00

CPC分类号： G01J3/12 ,  G01B11/0625 ,  G01B11/0641 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/42 ,  G01J4/00

摘要： The invention comprises an apparatus for characterizing a thin film using the reflected spectrum of the film. The apparatus uses two toroidal mirrors in an optical relay to direct light onto the thin film and to direct reflected light from the film to a spectrophotometer. A computer then analyzes the reflected spectrum to characterize the optical properties of the thin film. The optical relay allows for a range of angles of incidence upon the sample, and has no chromatic aberration.

摘要翻译： 本发明包括使用该膜的反射光谱来表征薄膜的装置。 该设备在光学继电器中使用两个环形反射镜来将光引导到薄膜上并将来自膜的反射光引导到分光光度计。 然后，计算机分析反射光谱以表征薄膜的光学性质。 光学继电器允许在样品上的入射角范围，并且没有色差。