公开(公告)号：US08044348B2

公开(公告)日：2011-10-25

申请号：US12642064

申请日：2009-12-18

Applicant: Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins ,  Serguel Savtchenko

Inventor： Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins ,  Serguel Savtchenko

IPC: H01J49/26 ,  H01J49/04

CPC classification number: H01J49/10 ,  H01J49/24 ,  Y10S438/961

Abstract: An ion source and method for providing ionized particles to a molecular/atomic analyser, such as a mass spectrometer, are disclosed. The ion source includes a vessel defining a channel; a gas inlet extending from the gas source into the channel, for introducing a gas flow into the channel; a sample inlet extending into the channel for introducing sample within the channel; and an ionizer to ionize the sample in the channel. The vessel is sufficiently sealed to allow the channel to be pressurized, at a pressure in excess of 100 Torr. At least one gas source maintains the pressure of the channel at a pressure in excess of 100 Torr and the pressure exterior to the channel at a pressure in excess of 0.1 Torr and provides a gas flow that sweeps across the ionizer to guide and entrain ions from the ionizer to the outlet.

Abstract translation: 公开了用于向分子/原子分析仪（例如质谱仪）提供离子化颗粒的离子源和方法。 离子源包括限定通道的容器; 从气体源延伸到通道中的气体入口，用于将气流引入通道中; 延伸到通道中的样品入口，用于将样品引入通道内; 以及电离器，用于使通道中的样品电离。 容器被充分密封，以允许通道在超过100托的压力下被加压。 至少一个气体源将通道的压力保持在超过100托的压力和在超过0.1托的压力下的通道外部的压力，并提供扫过电离器以引导和夹带离子的气流 电离器到出口。

公开(公告)号：US20100171033A1

公开(公告)日：2010-07-08

申请号：US12642064

申请日：2009-12-18

Applicant: Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins ,  Serguel Savtchenko

Inventor： Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins ,  Serguel Savtchenko

IPC: H01J49/26 ,  H01J49/04

CPC classification number: H01J49/10 ,  H01J49/24 ,  Y10S438/961

Abstract: An ion source and method for providing ionized particles to a molecular/atomic analyser, such as a mass spectrometer, are disclosed. The ion source includes a vessel defining a channel; a gas inlet extending from the gas source into the channel, for introducing a gas flow into the channel; a sample inlet extending into the channel for introducing sample within the channel; and an ionizer to ionize the sample in the channel. The vessel is sufficiently sealed to allow the channel to be pressurized, at a pressure in excess of 100 Torr. At least one gas source maintains the pressure of the channel at a pressure in excess of 100 Torr and the pressure exterior to the channel at a pressure in excess of 0.1 Torr and provides a gas flow that sweeps across the ionizer to guide and entrain ions from the ionizer to the outlet.

Abstract translation: 公开了用于向分子/原子分析仪（例如质谱仪）提供离子化颗粒的离子源和方法。 离子源包括限定通道的容器; 从气体源延伸到通道中的气体入口，用于将气流引入通道中; 延伸到通道中的样品入口，用于将样品引入通道内; 以及电离器，用于使通道中的样品电离。 容器被充分密封，以允许通道在超过100托的压力下被加压。 至少一个气体源将通道的压力保持在超过100托的压力和在超过0.1托的压力下的通道外部的压力，并提供扫过电离器以引导和夹带离子的气流 电离器到出口。

公开(公告)号：US07728292B2

公开(公告)日：2010-06-01

申请号：US11467720

申请日：2006-08-28

Applicant: Charles Jolliffe ,  Lisa Cousins ,  Gholamreza Javahery

Inventor： Charles Jolliffe ,  Lisa Cousins ,  Gholamreza Javahery

IPC: G01N23/00 ,  G21K7/00

CPC classification number: H01J49/025 ,  H01J49/0095

Abstract: An ion detector includes collision surfaces for converting both positively and negatively charged ions into emitted secondary electrons. Secondary electrons may be detected using an electron detector, than may, for example include an electron multiplier. Conveniently, secondary electrons (or electrons emitted by the multiplier) may be detected using an electron pulse counter.

Abstract translation: 离子检测器包括用于将正电荷和带负电荷的离子转换成发射的二次电子的碰撞表面。 可以使用电子检测器检测二次电子，例如可以包括电子倍增器。 方便地，可以使用电子脉冲计数器检测二次电子（或乘法器发射的电子）。

公开(公告)号：US07687771B2

公开(公告)日：2010-03-30

申请号：US11652649

申请日：2007-01-12

Applicant: Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins ,  Ilia Tomski

Inventor： Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins ,  Ilia Tomski

IPC: H01J49/04 ,  H01J49/22

CPC classification number: G01N30/7233 ,  H01J49/04 ,  H01J49/067 ,  H01J49/107

Abstract: An interface for mass spectrometers. The interface uses non coaxial sampling pathways of the analyte ion beam prior to entering the entrance of a mass spectrometer for decreasing chemical background, and can be done in such a way as to permit multiple sprayers, increasing sample throughput and sensitivity for LC/MS (liquid chromatography/MS). The interface includes an ion source having an exit from which a beam of analyte ions are emitted, a curtain plate and an aperture in the curtain plate member, an orifice plate having an orifice therein. The orifice plate is being spaced from the curtain plate member defining a flow passageway therebetween, and the aperture in the orifice plate is aligned with a sample entrance to a first vacuum stage of a mass spectrometer maintained substantially lower than atmospheric pressure. The aperture in the curtain plate member is non coaxially aligned with the orifice in the orifice plate and the interface includes a gas flow mechanism for directing a counter flow gas into the flow passageway.

公开(公告)号：US20090065692A1

公开(公告)日：2009-03-12

申请号：US12230949

申请日：2008-09-08

Applicant: Gholamreza Javahery ,  Lisa Cousins ,  Charles Jolliffe

Inventor： Gholamreza Javahery ,  Lisa Cousins ,  Charles Jolliffe

IPC: B01D59/44

CPC classification number: H01J49/0045

Abstract: A high pressure collision cell for use in a mass spectrometer. The high pressure collision cell has a cell length L selected to be in a range such that upon application of voltages to a pair of opposed elongate electrically conducting electrodes there is produced an electric field of sufficient strength across the collision cell length L in to aid in directing ions entering the collision cell to along a transverse flow axis. The pressure in the collision cell is maintained in a range from about 50 mTorr to 1000 mTorr and wherein the collision cell length L and the pressure are selected such that a target thickness, defined as a product of the collision cell length L and the pressure, is maintained in a range from about 0.2 to about 2 mm-Torr.

Abstract translation: 用于质谱仪的高压碰撞池。 高压碰撞池具有选择为在一定范围内的电池长度L，使得在向一对相对的细长导电电极施加电压时，产生跨越冲击电池长度L的足够强度的电场，以帮助 引导进入碰撞池的离子沿着横向流动轴。 碰撞池中的压力保持在约50mTorr至1000mTorr的范围内，并且其中选择碰撞池长度L和压力，使得定义为碰撞池长度L和压力的乘积的目标厚度， 维持在约0.2至约2mm-Torr的范围内。

公开(公告)号：US07091477B2

公开(公告)日：2006-08-15

申请号：US10864106

申请日：2004-06-09

Applicant: Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins

Inventor： Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins

IPC: B01D59/44 ,  H01J49/00

CPC classification number: H01J49/0422 ,  H01J49/04 ,  H01J49/044 ,  H01J49/0468 ,  H01J49/06 ,  H01J49/26 ,  Y10T436/24

Abstract: A mass spectrometer interface, having improved sensitivity and reduced chemical background, is disclosed. The mass spectrometer interface provides improved desolvation, chemical selectivity and ion transport. A flow of partially solvated ions is transported along a tortuous path into a region of disturbance of flow, where ions and neutral molecules collide and mix. Thermal energy is applied to the region of disturbance to promote liberation of at least some of the ionized particles from any attached impurities, thereby increasing the concentration of the ionized particles having the characteristic m/z ratios in the flow. Molecular reactions and low pressure ionization methods can also be performed for selective removal or enhancement of particular ions.

公开(公告)号：US07049584B1

公开(公告)日：2006-05-23

申请号：US11184387

申请日：2005-07-19

Applicant: Craig M. Whitehouse ,  David G. Welkie ,  Gholamreza Javahery ,  Lisa Cousins ,  Sergey Rakov

Inventor： Craig M. Whitehouse ,  David G. Welkie ,  Gholamreza Javahery ,  Lisa Cousins ,  Sergey Rakov

IPC: H01J49/26

CPC classification number: H01J49/147 ,  H01J49/0054

Abstract: Apparatus and methods are provided that enable the interaction of low-energy electrons and positrons with sample ions to facilitate electron capture dissociation (ECD) and positron capture dissociation (PCD), respectively, within multipole ion guide structures. It has recently been discovered that fragmentation of protonated ions of many biomolecules via ECD often proceeds along fragmentation pathways not accessed by other dissociation methods, leading to molecular structure information not otherwise easily obtainable. However, such analyses have been limited to expensive Fourier transform ion cyclotron resonance (FTICR) mass spectrometers; the implementation of ECD within commonly-used multipole ion guide structures is problematic due to the disturbing effects of RF fields within such devices. The apparatus and methods described herein successfully overcome such difficulties, and allow ECD (and PCD) to be performed within multipole ion guides, either alone, or in combination with conventional ion fragmentation methods. Therefore, improved analytical performance and functionality of mass spectrometers that utilize multipole ion guides are provided.

Abstract translation: 提供了能够使低能电子和正电子与样品离子相互作用的装置和方法，以便分别在多极离子导向结构内的电子俘获解离（ECD）和正电子俘获解离（PCD）。 最近已经发现，许多生物分子通过ECD的质子化离子的碎裂通常沿着不被其它解离方法接近的断裂途径进行，导致分子结构信息不容易获得。 然而，这种分析仅限于昂贵的傅里叶变换离子回旋共振（FTICR）质谱仪; 在常用的多极离子导向结构中的ECD的实现是由于RF场在这种装置内的干扰效应而成问题的。 本文描述的装置和方法成功地克服了这些困难，并且允许ECD（和PCD）在多极离子导向器中单独进行，或者与传统的离子碎裂方法组合进行。 因此，提供了利用多极离子导向器的质谱仪的改进的分析性能和功能性。

公开(公告)号：US06919562B1

公开(公告)日：2005-07-19

申请号：US10448477

申请日：2003-05-30

Applicant: Craig M. Whitehouse ,  David G. Welkie ,  Gholamreza Javahery ,  Lisa Cousins ,  Sergey Rakov

Inventor： Craig M. Whitehouse ,  David G. Welkie ,  Gholamreza Javahery ,  Lisa Cousins ,  Sergey Rakov

IPC: H01J49/26

CPC classification number: H01J49/147 ,  H01J49/0054

Abstract: Apparatus and methods are provided that enable the interaction of low-energy electrons and positrons with sample ions to facilitate electron capture dissociation (ECD) and positron capture dissociation (PCD), respectively, within multipole ion guide structures. It has recently been discovered that fragmentation of protonated ions of many biomolecules via ECD often proceeds along fragmentation pathways not accessed by other dissociation methods, leading to molecular structure information not otherwise easily obtainable. However, such analyses have been limited to expensive Fourier transform ion cyclotron resonance (FTICR) mass spectrometers; the implementation of ECD within commonly-used multipole ion guide structures is problematic due to the disturbing effects of RF fields within such devices. The apparatus and methods described herein successfully overcome such difficulties, and allow ECD (and PCD) to be performed within multipole ion guides, either alone, or in combination with conventional ion fragmentation methods. Therefore, improved analytical performance and functionality of mass spectrometers that utilize multipole ion guides are provided.

Abstract translation: 提供了能够使低能电子和正电子与样品离子相互作用的装置和方法，以便分别在多极离子导向结构内的电子俘获解离（ECD）和正电子俘获解离（PCD）。 最近已经发现，许多生物分子通过ECD的质子化离子的碎裂通常沿着不被其它解离方法接近的断裂途径进行，导致分子结构信息不容易获得。 然而，这种分析仅限于昂贵的傅里叶变换离子回旋共振（FTICR）质谱仪; 在常用的多极离子导向结构中的ECD的实现是由于RF场在这种装置内的干扰效应而成问题的。 本文描述的装置和方法成功地克服了这些困难，并且允许ECD（和PCD）在多极离子导向器中单独进行，或者与传统的离子碎裂方法组合进行。 因此，提供了利用多极离子导向器的质谱仪的改进的分析性能和功能性。

公开(公告)号：US20050035287A1

公开(公告)日：2005-02-17

申请号：US10864106

申请日：2004-06-09

Applicant: Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins

Inventor： Charles Jolliffe ,  Gholamreza Javahery ,  Lisa Cousins

IPC: H01J49/04 ,  H01J49/26 ,  H01J49/42

CPC classification number: H01J49/0422 ,  H01J49/04 ,  H01J49/044 ,  H01J49/0468 ,  H01J49/06 ,  H01J49/26 ,  Y10T436/24

Abstract: A mass spectrometer interface, having improved sensitivity and reduced chemical background, is disclosed. The mass spectrometer interface provides improved desolvation, chemical selectivity and ion transport. A flow of partially solvated ions is transported along a tortuous path into a region of disturbance of flow, where ions and neutral molecules collide and mix. Thermal energy is applied to the region of disturbance to promote liberation of at least some of the ionized particles from any attached impurities, thereby increasing the concentration of the ionized particles having the characteristic m/z ratios in the flow. Molecular reactions and low pressure ionization methods can also be performed for selective removal or enhancement of particular ions.

Abstract translation: 公开了具有改进的灵敏度和降低的化学背景的质谱仪界面。 质谱仪界面提供改进的去溶剂化学选择性和离子迁移。 部分溶剂化离子的流动沿曲折路径输送到流动扰动区域，其中离子和中性分子碰撞和混合。 热能被施加到扰动区域以促进至少一些离子化颗粒从任何附着的杂质的释放，从而增加流动中具有特征m / z比的离子化颗粒的浓度。 还可以进行分子反应和低压离子化方法以选择性去除或增强特定的离子。