公开(公告)号：US20200377942A1

公开(公告)日：2020-12-03

申请号：US16998414

申请日：2020-08-20

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20200255894A1

公开(公告)日：2020-08-13

申请号：US16736323

申请日：2020-01-07

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20200149090A1

公开(公告)日：2020-05-14

申请号：US16519863

申请日：2019-07-23

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Michael Schnall-Levin

IPC: C12Q1/6806 ,  B01L3/00 ,  B01J19/00 ,  C12N15/10

Abstract: This disclosure provides microwell capsule array devices. The microwell capsule array devices are generally capable of performing one or more sample preparation operations. Such sample preparation operations may be used as a prelude to one more or more analysis operations. For example, a device of this disclosure can achieve physical partitioning and discrete mixing of samples with unique molecular identifiers within a single unit in preparation for various analysis operations. The device may be useful in a variety of applications and most notably nucleic-acid-based sequencing, detection and quantification of gene expression and single-cell analysis.

公开(公告)号：US10557158B2

公开(公告)日：2020-02-11

申请号：US16228362

申请日：2018-12-20

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Kamila Belhocine

IPC: C12P19/34 ,  C40B20/04 ,  C12Q1/6806

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US20190203262A1

公开(公告)日：2019-07-04

申请号：US16242962

申请日：2019-01-08

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Donald Masquelier ,  Serge Saxonov ,  Landon Merrill ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/6806 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722 ,  C12N15/1006 ,  C12N15/1065 ,  C12Q2535/122 ,  C12Q2563/159

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20190177788A1

公开(公告)日：2019-06-13

申请号：US16231185

申请日：2018-12-21

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/519

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20190169666A1

公开(公告)日：2019-06-06

申请号：US16228362

申请日：2018-12-20

Applicant: 10X GENOMICS, INC.

Inventor： Paul Hardenbol ,  Pranav Patel ,  Benjamin Hindson ,  Paul William Wyatt ,  Keith Bjornson ,  Indira Wu ,  Kamila Belhocine

IPC: C12P19/34 ,  C12Q1/6806 ,  C40B20/04

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

公开(公告)号：US10208343B2

公开(公告)日：2019-02-19

申请号：US15831726

申请日：2017-12-05

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6804

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20180274027A1

公开(公告)日：2018-09-27

申请号：US15850241

申请日：2017-12-21

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Mirna Jarosz

IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20180142292A1

公开(公告)日：2018-05-24

申请号：US15872499

申请日：2018-01-16

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Grace Zheng ,  Philip Belgrader

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.