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134 条记录 (耗时 0.033 秒)

    Electrophoretic methods for spatial analysis
    2.
    发明授权
    Electrophoretic methods for spatial analysis 有权

    公开(公告)号：US11768175B1

    公开(公告)日：2023-09-26

    申请号：US17192536

    申请日：2021-03-04

    申请人： 10x Genomics, Inc.

    发明人： Hanyoup Kim ,  Augusto Manuel Tentori ,  Rajiv Bharadwaj

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44713

    摘要： The present disclosure provides electrophoretic systems, methods and compositions for spatial analysis, which can serve to magnify or demagnify spatial resolution of analytes of interest that are captured using electrophoresis. Some implementations can use a diverging or converging electric field in an electrophoretic capture system. Such a divergent or convergent electric field, as opposed to a parallel electric field, can be generated by, for example, utilizing different sizes of electrodes associated with or imbedded in substrates. Also provided herein are electrophoretic systems, methods and compositions for spatial analysis, which can serve to selectively migrate one or more analytes from a region of interest in the biological sample for capture using electrophoresis.

    Systems and methods for inferring cell status
    6.
    发明授权
    Systems and methods for inferring cell status 有权

    公开(公告)号：US11636921B2

    公开(公告)日：2023-04-25

    申请号：US16698612

    申请日：2019-11-27

    申请人： 10X Genomics, Inc.

    发明人： Michael Schnall-Levin ,  Rajiv Bharadwaj ,  Zahra Kamila Belhocine ,  Andrew D. Price ,  Yifeng Yin ,  Vijay Kumar Sreenivasa Gopalan ,  Zeljko Jovan Dzakula

    IPC分类号： G16B30/00 ,  G16H10/40 ,  G16H20/00

    摘要： Systems and methods for inferring a status of a cell population are provided. Described techniques allow deconvolving a first clonal population comprising a first plurality of cells of a species, wherein nucleic acid sequence reads from each cell in the first plurality of cells are obtained. The nucleic acid sequence reads are mapped into bins representing portions of a reference genome, and a pattern of sequence read counts for each cell across the multiple bins is used to assign a cell to a group, thereby inferring a mitotic status of the cell. The assignment of nucleic acid sequence reads into bins is also be used for segregating cells into classes based on a status of a certain biological marker in each cell. Comparison of sequence read counts for a subset of bins across the cell classes allows evaluating effect of a compound on a cell status.

    Microfluidic systems and methods of use
    9.
    发明授权
    Microfluidic systems and methods of use 有权

    公开(公告)号：US11084036B2

    公开(公告)日：2021-08-10

    申请号：US16180356

    申请日：2018-11-05

    申请人： 10X GENOMICS, INC.

    发明人： Rajiv Bharadwaj ,  Kevin Ness ,  Tobias Daniel Wheeler

    IPC分类号： B01L3/00 ,  G01N1/28 ,  G01N33/487

    摘要： Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.