公开(公告)号：US20190001326A1

公开(公告)日：2019-01-03

申请号：US16064064

申请日：2016-12-19

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Evan D. Brutinel ,  Joseph D. Rule ,  Jonathan E. Janoski ,  Anthony F. Schultz

IPC: B01L3/00

Abstract: Sample partitioning devices and methods of making and using the same are described. A sample partitioning device includes a first film having an array of discrete stems each extending from a first major surface thereof, and a second film having an array of discrete wells formed into a second major surface thereof. The stems of the first film and the wells of the second film are mated with each other. The mated stems and wells are separable from each other, and during the removal of the stems from the wells, one or more voids are created inside the wells to suction an aqueous test sample into the wells.

公开(公告)号：US20180273887A1

公开(公告)日：2018-09-27

申请号：US15763744

申请日：2016-09-27

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Evan D. Brutinel ,  Jason W. Bjork ,  Adam J. Stanenas

IPC: C12M1/32 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/24

CPC classification number: C12M23/12 ,  C12M23/38 ,  C12M41/34 ,  C12M41/36 ,  C12Q1/04 ,  C12Q1/24

Abstract: In one aspect, the present disclosure provides a device. The device includes a body comprising a waterproof base, a waterproof coversheet attached to the base, and a channel disposed between the base and the coversheet. The channel has a perimeter and an opening that provides liquid access to the channel. A portion of the perimeter is defined by a waterproof seal. A dry first oxygen-scavenging reagent and an indicator reagent for detecting sulfate reduction by a sulfate-reducing bacterium are disposed in the device between the base and the coversheet. The waterproof base comprises a plurality of open microcompartment structures facing the coversheet.

公开(公告)号：US09987633B2

公开(公告)日：2018-06-05

申请号：US15309505

申请日：2015-06-08

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Stephen B. Roscoe ,  Myungchan Kang ,  Evan D. Brutinel ,  Jesse D. Miller ,  Renee K. Cline ,  Minghua Dai ,  Hsi-Chou Liu

IPC: B01L3/00 ,  G01N33/53

CPC classification number: B01L3/5029 ,  B01L3/502 ,  B01L2200/16 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/0478 ,  G01N33/5304

Abstract: Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.

公开(公告)号：US20180051313A1

公开(公告)日：2018-02-22

申请号：US15556664

申请日：2016-03-15

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Raj Rajagopal ,  Manjiri T. Kshirsagar ,  Evan D. Brutinel ,  James E. Aysta

IPC: C12Q1/24 ,  C12M1/12 ,  C12M1/26 ,  C12M1/34 ,  G01N1/10 ,  G01N1/40

CPC classification number: C12Q1/24 ,  B01D46/00 ,  C12M1/12 ,  C12M1/26 ,  C12M1/34 ,  C12Q1/04 ,  G01N1/10 ,  G01N1/405 ,  G01N2001/4088

Abstract: Methods of detecting microorganisms in a fluid sample are provided. The method includes providing a nonwoven article, providing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte, and contacting the fluid sample with the nonwoven article such that at least a portion of the at least one microorganism strain or target cellular analyte is bound to the nonwoven article. The nonwoven article includes a fibrous porous matrix and a concentration agent particles enmeshed in the fibrous porous matrix. The method further includes placing the microorganism strain- or target cellular analyte-bound nonwoven article in contact with at least one detection reagent and detecting the presence of the bound microorganism strain or bound target cellular analyte. Devices and kits containing the devices, for contacting a fluid sample with a nonwoven article, are also provided. The device includes a sample container, a filter holder, a nonwoven article, and an adaptor configured to interface the filter holder with a receptacle.