Abstract:
Guanidine-functionalized perlite particles are provided. Nonwoven articles are also provided, including a fibrous porous matrix and guanidine-functionalized perlite particles enmeshed in the fibrous porous matrix. Laminated articles are additionally provided, including a first substrate and a second substrate sealed to the first substrate along at least a portion of a perimeter of the first substrate. The laminated article further includes guanidine-functionalized perlite particles disposed between the first substrate and the second substrate. Methods of detecting microorganisms or target cellular analytes in a fluid sample using guanidine-functionalized particles or laminated articles are also provided.
Abstract:
Porous articles are provided that include a fibrous porous matrix and porous polymeric particles. The porous polymeric particles are distributed throughout the fibrous porous polymeric matrix. The porous article can be used to prepare a separation device or a system that includes the separation device. The porous articles can be used for the separation of a target material such as a microorganism (i.e., cellular analyte) from a sample.
Abstract:
Kits and systems for isolating microorganisms from a sample, the sample including sample matrix and microorganisms, the kit including concentration agent; and a system for isolating microorganisms from a sample.
Abstract:
Guanidine-functionalized particles and methods of making and using such particles are provided, such as guanidine-functionalized metal silicate particles. A nonwoven article is also provided including a porous fibrous nonwoven matrix and guanidine-functionalized metal silicate particles enmeshed within the porous fibrous nonwoven matrix. The nonwoven article may be used, for instance, for removing microorganisms from an aqueous sample by contacting a fluid sample with the nonwoven article such that at least a portion of the microorganisms are removed from the fluid sample.
Abstract:
The present disclosure provides a monitoring device comprising a test composition, a test element comprising a test portion to which the test composition is releasably adhered, a detection reagent, and a container comprising a first end with an opening and a second end opposite the first end. The test composition comprises a predetermined quantity of tracer analyte. The container is configured to receive the test portion and configured to be operationally coupled to an analytical instrument. The tracer analyte and the detection reagent each are capable of participating in one or more chemical reaction that results in the formation of a detectable product. A method of using the monitoring device to assess the efficacy of a washing process is also provided.
Abstract:
Porous devices are provided that include a particle-containing fibrous porous matrix and a fluid absorbed in the particle-containing porous fibrous nonwoven matrix. The particle-containing fibrous porous matrix includes a porous fibrous nonwoven matrix containing first polyolefin fibers, second polyolefin fibers including poly(ethylene), and fiberglass fibers; and microorganism-binding particles. The microorganism-binding particles are enmeshed in said porous fibrous nonwoven matrix. Methods of debridement are provided including providing a porous device including a particle-containing fibrous porous matrix and wiping a wound or an area of skin with the device; and providing a porous device including a porous fibrous nonwoven matrix and wiping a wound with the device. Further, kits are provided including a sterile package containing at least one porous device, and typically also instructions for debridement.
Abstract:
A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.
Abstract:
A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising (1) a porous fibrous nonwoven matrix and (2) a plurality of particles of at least one concentration agent that comprises a metal silicate, the particles being enmeshed in the porous fibrous nonwoven matrix; (b) providing a sample comprising at least one target cellular analyte; (c) contacting the concentration device with the sample such that at least a portion of the at least one target cellular analyte is bound to or captured by the concentration device; and (d) detecting the presence of at least one bound target cellular analyte.
Abstract:
The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.
Abstract:
The present invention provides methods to concentrate cells onto microparticles, to concentrate the microparticles, and to detect the cells. The present invention also includes unitary sample preparation and detection devices to be used in accordance with the methods.